scholarly journals Experimental Investigation of a Pilot-Scale Concerning Ex-Situ Bioremediation of Petroleum Hydrocarbons Contaminated Soils

2021 ◽  
Vol 13 (15) ◽  
pp. 8165
Author(s):  
Valer Micle ◽  
Ioana Monica Sur
Keyword(s):  
Contaminated Soils ◽  
Petroleum Hydrocarbons ◽  
Petroleum Hydrocarbon ◽  
Industrial Area ◽  
Pilot Scale ◽  
Soil Samples ◽  
Point Of View ◽  
Ex Situ ◽  
Nutrient Contents ◽  
Aeration Time

The soil samples were taken from the site of a former oil products depot from an industrial area (Romania). The soil samples taken were analyzed from a physical and chemical point of view: texture, pH, soil micronutrient content, metals concentration and petroleum hydrocarbon concentration (PHCs). The soil contaminated with total petroleum hydrocarbon (TPH (4280 mg kg−1) was disposed in the form of a pile (L × W × H: 3000 × 1400 × 500 mm). Experiments on a pilot-scale were conducted over 12 weeks at constant pH (7.5–8), temperature (22–32 °C), nutrient contents C/N/P ratio 100/10/1, soil aeration time (8 h/day) and moisture (30%). Samples were taken every two weeks for the monitoring of the TPH and the microorganisms content. During the experiment, microorganisms were added (Pseudomonas and Bacillus) every two weeks. Results of the analyses regarding the concentration of PHCs were revealed a linear decrease of the concentration of PHCs after only two weeks of treatment. This decrease in concentration was also achieved in the following weeks. Following the analysis performed on the model at the pilot scale regarding the depollution process, it can be concluded that a soil contaminated with petroleum hydrocarbons can be efficiently depolluted by performing an aeration of 8 h/day, adding microorganisms Pseudomonas and Bacillus to ensure the conditions for increasing in the total number of germs (colony forming units–CFU) from 151 × 105 to 213 × 107 CFU g−1 soil, after 12 weeks of soil treatment—the depollution efficiency achieved is 83%.

scholarly journals Experimental Investigation of a Pilot-Scale Concerning Ex-Situ Bioremediation of Petroleum Hydrocarbons Contaminated Soils

2021 ◽  
Author(s):  
Valer Micle ◽  
Ioana Sur
Keyword(s):  
Contaminated Soils ◽  
Petroleum Hydrocarbons ◽  
Industrial Area ◽  
Pilot Scale ◽  
Soil Samples ◽  
Point Of View ◽  
Ex Situ ◽  
Nutrient Contents ◽  
Aeration Time ◽  
Physical And Chemical

Abstract The soil samples were taken from the site of a former oil products depot from an industrial area (Romania). The soil samples taken were analyzed from a physical and chemical point of view: texture, ph, soil micronutrient content, metals concentration and petroleum hydrocarbon concentration (PHCs). The soil contaminated with TPH (4280 mgkg-1) was disposed in the form of a pile (LxWxH:3000x1400x500 mm). Experiments a pilot-scale were conducted over 12 weeks at constant pH (7.5–8), temperature (22–32oC), nutrient contents C/N/P ratio 100:10:1, soil aeration time (8 hour/day) and moisture (30%). Samples were taken every two weeks for the monitoring of the TPH and the microorganisms content. During experiment every two weeks were added microoganisms (Pseudomonas and Bacillus). Results of the analyzes regarding the concentration of PHCs were revelead a linear decrease of the concentration of PHCs after only two weeks of treatment. This decrease in concentration was also achieved in the following weeks. Following the analysis performed on the model at the pilot scale regarding the depollution process, it can be concluded that a soil contaminated with petroleum hydrocarbons can be efficiently depolluted by performing an aeration of 8 h/day, adding microorganisms Pseudomonas and Bacillus to ensure the conditions for increasing in the total number of germs (colony forming units–CFU) from 151x105 to 213x107 CFU/gram of soil, after 12 weeks of soil treatment - the depollution efficiency achieved is 83%.

scholarly journals Bioremediation of Soil Contaminated with Diesel using Biopile system

2018 ◽  
Vol 14 (3) ◽  
pp. 48-56
Author(s):  
Noor Mohsen Jabbar ◽  
Estabriq Hasan Kadhim ◽  
Alaa Kareem Mohammed
Keyword(s):  
Contaminated Soil ◽  
Contaminated Soils ◽  
Petroleum Hydrocarbons ◽  
Bacterial Culture ◽  
Atmospheric Temperature ◽  
Pilot Scale ◽  
Ex Situ ◽  
Total Petroleum Hydrocarbons ◽  
Bacterial Inoculum ◽  
Amended Soil

This study was focused on biotreatment of soil which polluted by petroleum compounds (Diesel) which caused serious environmental problems. One of the most effective and promising ways to treat diesel-contaminated soil is bioremediation. It is a choice that offers the potential to destroy harmful pollutants using biological activity. The capability of mixed bacterial culture was examined to remediate the diesel-contaminated soil in bio piling system. For fast ex-situ treatment of diesel-contaminated soils, the bio pile system was selected. Two pilot scale bio piles (25 kg soil each) were constructed containing soils contaminated with approximately 2140 mg/kg total petroleum hydrocarbons (TPHs). The amended soil: (contaminated soil with the addition of nutrients and bacterial inoculum), where the soil was mixed with 1.5% of sawdust, then supplied with the necessary nutrients and watered daily to provide conditions promoting microorganism growth. Unamended soil was prepared as a control (contaminated soil without addition).  Both systems were equipped with oxygen to provide aerobic conditions, incubated at atmospheric temperature and weekly sampling within 35 days. Overall 75% of the total petroleum hydrocarbons were removed from the amended soil and 38 % of the control soil at the end of study period. The study concluded that ex-situ experiment (Bio pile) is a preferable, economical, and environmentally friendly procedure, thus representing a good option for the treatment of soil contaminated with diesel.

scholarly journals Bioremediation of Soil Contaminated with Diesel using Biopile system

2018 ◽  
Vol 14 (3) ◽  
pp. 48-56 ◽  
Author(s):  
Noor Mohsen Jabbar ◽  
Estabriq Hasan Kadhim ◽  
Alaa Kareem Mohammed
Keyword(s):  
Contaminated Soil ◽  
Contaminated Soils ◽  
Petroleum Hydrocarbons ◽  
Bacterial Culture ◽  
Atmospheric Temperature ◽  
Pilot Scale ◽  
Ex Situ ◽  
Total Petroleum Hydrocarbons ◽  
Bacterial Inoculum ◽  
Amended Soil

This study was focused on biotreatment of soil which polluted by petroleum compounds (Diesel) which caused serious environmental problems. One of the most effective and promising ways to treat diesel-contaminated soil is bioremediation. It is a choice that offers the potential to destroy harmful pollutants using biological activity. The capability of mixed bacterial culture was examined to remediate the diesel-contaminated soil in bio piling system. For fast ex-situ treatment of diesel-contaminated soils, the bio pile system was selected. Two pilot scale bio piles (25 kg soil each) were constructed containing soils contaminated with approximately 2140 mg/kg total petroleum hydrocarbons (TPHs). The amended soil: (contaminated soil with the addition of nutrients and bacterial inoculum), where the soil was mixed with 1.5% of sawdust, then supplied with the necessary nutrients and watered daily to provide conditions promoting microorganism growth. Unamended soil was prepared as a control (contaminated soil without addition).  Both systems were equipped with oxygen to provide aerobic conditions, incubated at atmospheric temperature and weekly sampling within 35 days. Overall 75% of the total petroleum hydrocarbons were removed from the amended soil and 38 % of the control soil at the end of study period. The study concluded that ex-situ experiment (Bio pile) is a preferable, economical, and environmentally friendly procedure, thus representing a good option for the treatment of soil contaminated with diesel.

scholarly journals Biodegradation Potentials of Aspergillus sydowii and Fusarium lichenicola on Total Petroleum Hydrocarbon in an Oilfield Wastewater in Rivers State

2020 ◽  
pp. 1-7
Author(s):  
Williams, Janet Olufunmilayo ◽  
Aleruchi Owhonka
Keyword(s):  
Mixed Culture ◽  
Contaminated Soils ◽  
Petroleum Hydrocarbons ◽  
Petroleum Hydrocarbon ◽  
Total Petroleum Hydrocarbon ◽  
Total Petroleum Hydrocarbons ◽  
Aspergillus Sydowii ◽  
Significant Difference ◽  
Set Up ◽  
Rivers State

This study investigated the potential of Aspergillus sydowii and Fusarium lichenicola as mixed cultures in the biodegradation of Total Petroleum Hydrocarbons TPHs in oilfield wastewater. Oilfield wastewater was collected from an onshore oil producing platform and biodegradation of total petroleum hydrocarbons was investigated using standard methods. Fungi were isolated from oilfield wastewater contaminated soils obtained from the vicinity of the oil producing platform. Experimental control set-up and treatment with mixed culture of fungal isolates were periodically analyzed on days 7 and 21 intervals for total petroleum hydrocarbon degradation using Gas Chromatography (GC). The total amount of TPHs on day 1 recorded 381. 871 mg/l.  The amount of TPHs on days 7 and 21 in the mixed culture of fungi was 108.975 mg/l and 21.105 mg/l respectively while TPHs in control was 342.891 mg/l and 240.749 mg/l respectively. There was a significant difference between the mixed culture and the control on days 7 and 21 at p≤0.05. The results therefore revealed actual and significant reduction of TPHs in the mixed culture. In addition, there was clearance of n-alkanes by the mixed culture. This suggests that fungi have great potentials in biodegradation of TPHs and in remediation of TPH contaminated environments.

scholarly journals Soil Bioremediation: Overview of Technologies and Trends

Energies ◽  
2020 ◽  
Vol 13 (18) ◽  
pp. 4664
Author(s):  
Israel Gonçalves Sales da Silva ◽  
Fabíola Carolina Gomes de Almeida ◽  
Nathália Maria Padilha da Rocha e Silva ◽  
Alessandro Alberto Casazza ◽  
Attilio Converti ◽  
...  
Keyword(s):  
Contaminated Soils ◽  
Petroleum Hydrocarbons ◽  
Ex Situ ◽  
Soil Bioremediation ◽  
Application Of Surfactants ◽  
Agricultural Pesticides ◽  
Advantages And Disadvantages ◽  
Microbiological Characteristics ◽  
Extent Of Damage

Petroleum hydrocarbons, heavy metals and agricultural pesticides have mutagenic, carcinogenic, immunotoxic and teratogenic effects and cause drastic changes in soil physicochemical and microbiological characteristics, thereby representing a serious danger to health and environment. Therefore, soil pollution urgently requires the application of a series of physicochemical and biological techniques and treatments to minimize the extent of damage. Among them, bioremediation has been shown to be an alternative that can offer an economically viable way to restore polluted areas. Due to the difficulty in choosing the best bioremediation technique for each type of pollutant and the paucity of literature on soil bioremediation enhanced by the use of specific additives, we reviewed the main in situ and ex situ methods, their current properties and applications. The first section discusses the characteristics of each class of pollutants in detail, while the second section presents current bioremediation technologies and their main uses, followed by a comparative analysis showing their respective advantages and disadvantages. Finally, we address the application of surfactants and biosurfactants as well as the main trends in the bioremediation of contaminated soils.

scholarly journals The use of vinasse as an amendment to ex-situ bioremediation of soil and groundwater contaminated with diesel oil

2009 ◽  
Vol 52 (4) ◽  
pp. 1043-1055 ◽  
Author(s):  
Adriano Pinto Mariano ◽  
Sérgio Henrique Rezende Crivelaro ◽  
Dejanira de Franceschi de Angelis ◽  
Daniel Marcos Bonotto
Keyword(s):  
Contaminated Soils ◽  
Petroleum Hydrocarbons ◽  
Microbial Population ◽  
Diesel Oil ◽  
Ex Situ ◽  
Total Petroleum Hydrocarbons ◽  
Co2 Production ◽  
Soil Bioremediation ◽  
Negative Effects ◽  
Labile Carbon

This work investigated the possibility of using vinasse as an amendment in ex-situ bioremediation processes. Groundwater and soil samples were collected at petrol stations. The soil bioremediation was simulated in Bartha biometer flasks, used to measure the microbial CO2 production, during 48 days, where vinasse was added at a concentration of 33 mL.Kg-1of soil. Biodegradation efficiency was also measured by quantifying the total petroleum hydrocarbons (TPH) by gas chromatography. The groundwater bioremediation was carried out in laboratory experiments simulating aerated (bioreactors) and not aerated (BOD flasks) conditions. In both the cases, the concentration of vinasse was 5 % (v/v) and different physicochemical parameters were evaluated during 20 days. Although an increase in the soil fertility and microbial population were obtained with the vinasse, it demonstrated not to be adequate to enhance the bioremediation efficiency of diesel oil contaminated soils. The addition of the vinasse in the contaminated groundwaters had negative effects on the biodegradation of the hydrocarbons, since vinasse, as a labile carbon source, was preferentially consumed.

scholarly journals Phytoremediation of hydrocarbon-contaminated soils with emphasis on the effect of petroleum hydrocarbons on the growth of plant species

2009 ◽  
Vol 89 (1) ◽  
pp. 21-29 ◽  
Author(s):  
Ravanbakhsh Shirdam ◽  
Ali Daryabeigi Zand ◽  
Gholamreza Nabi Bidhendi ◽  
Nasser Mehrdadi
Keyword(s):  
Plant Species ◽  
Contaminated Soil ◽  
Contaminated Soils ◽  
Petroleum Hydrocarbons ◽  
Petroleum Hydrocarbon ◽  
Green Technology ◽  
Hydrocarbon Contamination ◽  
Total Petroleum Hydrocarbons ◽  
Control Treatment ◽  
Polluted Soils

To date, many developing countries such as Iran have almost completely abandoned the idea of decontaminating oil-polluted soils due to the high costs of conventional (physical/chemical) soil remediation methods. Phytoremediation is an emerging green technology that can become a promising solution to the problem of decontaminating hydrocarbon-polluted soils. Screening the capacity of native tolerant plant species to grow on aged, petroleum hydrocarbon-contaminated soils is a key factor for successful phytoremediation. This study investigated the effect of hydrocarbon pollution with an initial concentration of 40 000 ppm on growth characteristics of sorghum (Sorghum bicolor) and common flax (Linum usitatissumum). At the end of the experiment, soil samples in which plant species had grown well were analyzed for total petroleum hydrocarbons (TPHs) removal by GC-FID. Common flax was used for the first time in the history of phytoremediation of oil-contaminated soil. Both species showed promising remediation efficiency in highly contaminated soil; however, petroleum hydrocarbon contamination reduced the growth of the surveyed plants significantly. Sorghum and common flax reduced TPHs concentration by 9500 and 18500 mg kg‑1, respectively, compared with the control treatment.

scholarly journals Surfactant Enhanced Bioremediation of Hydrocarbon Contaminated Soils Using Alkyl Polyglucoside

2021 ◽  
pp. 17-28
Author(s):  
C. E. Ezekiel ◽  
Leo C. Osuji ◽  
M. C. Onojake
Keyword(s):  
Contaminated Soils ◽  
Petroleum Hydrocarbons ◽  
Soil Samples ◽  
Hydrocarbon Contamination ◽  
Total Petroleum Hydrocarbons ◽  
Alkyl Polyglycosides ◽  
Enhanced Biodegradation ◽  
Enhanced Bioremediation ◽  
Reduction Percentage ◽  
Alkyl Polyglucoside

Bioremediation is an efficient and environmentally friendly method for the degradation of petroleum hydrocarbons in contaminated soils. This study investigated the effects of biosurfactant alkyl polyglycosides (APG) on enhanced biodegradation of petroleum hydrocarbon contaminated soils.  Three soil samples were contaminated with two different grades of crude oil (medium and Light). Alkyl polyglucoside was synthesised and subjected to FTIR for comfirmation of the product before it was applied in the remediation of contaminated soil. The alkyl polyglucoside is used as a treatment regime in the remediation of the hydrocarbon contamination in the three soil samples. Results of total petroleum hydrocarbons (TPH) before remediation with bio-surfactant showed that samples contaminated with medium crude for Eneka, Ozuoba and Rukpokwu were 15744.00 mg/kg, 11359.00 mg/kg and 11470.00 mg/kg respectively and after remediation reduced to 4276.00 mg/kg, 4265.00 mg/kg, and 3205.00 mg/kg, showing a reduction percentage of 72.84%, 62.44% and 72.05% respectively. Soil samples contaminated with light crude showed result of TPH of 11339.00 mg/kg, 10662 mg/kg and 10226 mg/kg and after remediation reduced to 2981 mg/kg, 3879 mg/kg, and 4245 mg/kg respectively showing a reduction percentage of 73.71%, 63.62 % and 58.49% respectively. The enhanced efficiency of the bio-surfactant at degrading total petroleum hydrocarbons was achieved as a result of the increased solubility thus improving the bioavailability of the hydrocarbons due to the action of the alkyl polyglucoside.

Lab-scale experimental investigation concerning ex-situ bioremediation of petroleum hydrocarbons-contaminated soils

2018 ◽  
Vol 27 (8) ◽  
pp. 692-705 ◽  
Author(s):  
Valer Micle ◽  
Ioana Monica Sur ◽  
Adriana Criste ◽  
Marin Senila ◽  
Erika Levei ◽  
...  
Keyword(s):  
Experimental Investigation ◽  
Contaminated Soils ◽  
Petroleum Hydrocarbons ◽  
Ex Situ
Sign in / Sign up

Export Citation Format

Share Document