Combined use of arbuscular mycorrhizal fungus and selenium fertilizer shapes microbial community structure and enhances organic selenium accumulation in rice grain

Elevated ozone (O3) generally affects microbial biomass and community structure in rhizosphere, but these effects are unclear in mycorrhizal plants because arbuscular mycorrhizal (AM) fungi often benefit microbial growth in the rhizosphere. Here, we investigate the effects of elevated O3 on microbial biomass and community structure in the rhizosphere of mycorrhizal snap bean (Phaseolus vulgaris L.) with different O3 sensitivity (R123: O3-tolerant plant; S156: O3-sensitive plant) based on the phospholipid fatty acids (PLFAs) method. Compared with ambient O3, elevated O3 significantly decreased mycorrhizal colonization rates in the 2 genotypes, especially in S156 plants. The wet masses of shoot and root were decreased by elevated O3 in the 2 genotypes independent of AM inoculation, but they were higher in the mycorrhizal plant than in the nonmycorrhizal plant independent of O3 concentration. Elevated O3 significantly decreased the relative proportion of specific fungal PLFAs in the nonmycorrhizal plant, but this effect disappeared in the mycorrhizal plant. The relative proportions of specific PLFAs of other microbial groups (Gram-positive, Gram-negative, and actinomycete) in the rhizosphere and all specific PLFAs in the hyphosphere were not affected by elevated O3 independent of AM inoculation. In the rhizosphere of the 2 genotypes, microbial community structure was changed by AM inoculation and elevated O3 as well as by their interaction; in the hyphosphere, however, microbial community structure was changed by elevated O3 only in R123 plants. It is concluded that AM inoculation can offset negative effect of elevated O3 on fungal biomass but seems to enhance shift of microbial community structure in rhizosphere under elevated O3.

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Simulated Nitrogen Deposition Causes a Decline of Intra- and Extraradical Abundance of Arbuscular Mycorrhizal Fungi and Changes in Microbial Community Structure in Northern Hardwood Forests

Ecosystems ◽

10.1007/s10021-010-9347-0 ◽

2010 ◽

Vol 13 (5) ◽

pp. 683-695 ◽

Cited By ~ 79

Author(s):

Linda T. A. van Diepen ◽

Erik A. Lilleskov ◽

Kurt S. Pregitzer ◽

R. Michael Miller

Keyword(s):

Community Structure ◽

Microbial Community ◽

Arbuscular Mycorrhizal Fungi ◽

Nitrogen Deposition ◽

Microbial Community Structure ◽

Mycorrhizal Fungi ◽

Arbuscular Mycorrhizal ◽

Hardwood Forests ◽

Northern Hardwood Forests ◽

Northern Hardwood

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Effect of arbuscular mycorrhizal fungi (AMF) and plant growth promoting rhizobacteria (PGPR) on microbial community structure of phenanthrene and pyrene contaminated soils using Illumina HiSeq sequencing

10.1101/839910 ◽

2019 ◽

Author(s):

Wen-bin Li ◽

Wei Li ◽

Li-jun Xing ◽

Shao-xia Guo

Keyword(s):

Community Structure ◽

Microbial Community ◽

Microbial Community Structure ◽

Tall Fescue ◽

Contaminated Soils ◽

Mycorrhizal Fungi ◽

Arbuscular Mycorrhizal ◽

Plant Growth Promoting Rhizobacteria ◽

Illumina Hiseq ◽

Plant Growth Promoting

AbstractIn order to determine the influence of arbuscular mycorrhizal fungi (AMF, Glomus versiforme) and plant growth promoting rhizobacteria (PGPR, Pseudomonas fluorescens, PS2-6) on degradation of phenanthrene (PHE) and pyrene (PYR) and the change of microbial community structure in soils planted with tall fescue (Festuca elata), four treatments were set up in phenanthrene (PHE) and pyrene (PYR) contamined soil: i.e., tall fescue (CK), AMF + tall fescue (GV), PGPR + tall fescue (PS) and AMF + PGPR + tall fescue (GVPS), PHE and PYR dissipation in the soil and accumulated in the tall fescue were investigated. Our results showed that highest removal percentage of PHE and PYR in contaminated soil as well as biomass of tall fescue were observed in GVPS. PHE and PYR accumulation by tall fescue roots were higher than shoots, the mycorrhizal status was best manifested in the roots of tall fescue inoculated with GVPS, and GVPS significantly increased the number of PGPR colonization in tall fescue rhizosphere soil. And paired-end Illumina HiSeq analysis of 16S rRNA and Internal Transcribed Spacer (ITS) gene amplicons were also employed to study change of bacterial and fungal communities structure in four treatments. GVPS positively affected the speices and abundance of bacteria and fungi in PHE and PYR contaminated soil, an average of 71,144 high quality bacterial 16S rDNA tags and 102,455 ITS tags were obtained in GVPS, and all of them were assigned to 6,327 and 825 operational taxonomic units (OTUs) at a 97% similarity, respectively. Sequence analysis revealed that Proteobacteria was the dominant bacterial phylum, Ascomycota was the dominant fungal phylum in all treatments, whereas Proteobacteria and Glomeromycota were the most prevalent bacterial and fungal phyla in GVPS, respectively. And in the generic level, Planctomyces is the richest bacterial genus, and Meyerozyma is the richest fungal genus in all treatments, whereas Sphingomona was the dominant bacterial genus, while the dominant fungi was Fusarium in GVPS. Overall, our findings revealed that application of AMF and PGPR had an effective role in improving the growth characteristics, root colonization of F. elata and soil microbial community structure in PHE and PYR contaminated soils, but no obvious in degradation efficiencies of PAHs as compared to the control.

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