Hemogasometry, cardiac biomarkers and blood metabolites in goats with experimentally induced acute ruminal lactic acidosis

ABSTRACT This study aimed to investigate the changes in the acid-base balance of sheep with experimentally induced acute ruminal lactic acidosis (ARA). Ten ewes orally received 15 grams of sucrose per kilogram of body mass. Arterial blood samples for blood gas analysis were obtained at the following intervals: before the induction of ARA (control), and 2, 4, 6, 8, 10, 12, 16, 20, 24, 28, 32, 36, 48, 72, 96, 120 and 144 hours after sucrose administration. Urine samples for pH measurement were obtained at the following times: -15 days, -7 days, and immediately before sucrose administration, then at 24, 48, 72, 96, 120 and 144 hours. Thereafter, both blood and urine samples were obtained on the 2nd, 3rd, and 4th following weeks. From 4 hours after the induction, elevation of the pH, bicarbonate and base excess on the arterial blood was observed. After 12 hours, the animals showed a decrease of these parameters, as well as urine acidification, which are symptomatic of metabolic acidosis. Within 28 hours, all parameters were normalized except the base excess, which only returned to normal after 72 hours. Despite the occurrence of acidemia, there was no need for medication and no animals died.

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Experimentally induced lactic acidosis in the goat

Australian Veterinary Journal ◽

10.1111/j.1751-0813.1987.tb09605.x ◽

1987 ◽

Vol 64 (12) ◽

pp. 367-370 ◽

Cited By ~ 18

Author(s):

G R CAO ◽

P B ENGLISH ◽

L J FILIPPICH ◽

S. INGLIS

Keyword(s):

Lactic Acidosis ◽

Experimentally Induced

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Experimentally induced hyperchloremic and dl-lactic acidosis in calves: An attempt to study the effects of oral rehydration on acid-base status

Journal of Dairy Science ◽

10.3168/jds.2012-6077 ◽

2013 ◽

Vol 96 (4) ◽

pp. 2464-2475 ◽

Cited By ~ 4

Author(s):

L. Schwedhelm ◽

D. Kirchner ◽

B. Klaus ◽

L. Bachmann

Keyword(s):

Lactic Acidosis ◽

Acid Base ◽

Oral Rehydration ◽

Acid Base Status ◽

Experimentally Induced

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Evaluation of serum cardiac biomarkers in sheep with acute lactic acidosis

Journal of the Hellenic Veterinary Medical Society ◽

10.12681/jhvms.15608 ◽

2018 ◽

Vol 68 (2) ◽

pp. 219 ◽

Cited By ~ 2

Author(s):

M. FARTASHVAND ◽

Y. HAJI-SADEGHI

Keyword(s):

Heart Rate ◽

Lactic Acidosis ◽

Respiratory Rate ◽

Rumen Fluid ◽

Venous Blood ◽

Cardiac Biomarkers ◽

Hydration Status ◽

Control Group ◽

Serum Samples ◽

Ruminal Ph

In this study we investigated the changes of cardiac injury biomarkers in serum samples from 200 sheep with acute ruminal lactic acidosis (ARLA) and 50 healthy controls belonging to the Ghezel breed. After clinical examination and recording of vital signs (heart rate, respiratory rate, rectal temperature and hydration status), rumen fluid and venous blood samples were collected. The pH of rumen fluid was determined using a paper tape pH-meter and lactic acid concentration of serum was measured using a commercially available method. Similarly, activities of AST, LDH, CK-MB and serum concentration of cTnI were measured using special commercial kits. According to the findings serum activities of AST (p = 0.007) and CK-MB (p = 0.002) in sheep with ARLA were significantly higher than in healthy animals. Serum LDH activity in the disease group was higher than in the control group, however this difference was statistically non-significant. cTnI concentration were 0.684 ± 0.03 ng/ml in sheep with ARLA, which was significantly higher than in healthy sheep (p = 0.000). There were significant negative correlations between ruminal pH and serum lactate levels, heart rate, respiratory rate and dehydration degree. The sheep with the lowest ruminal pH (3 cases with < 4.5) had the highest levels of cTnI (2.28 to 3.06 ng/mL), and all died. It can be concluded that lactic acidosis may cause some degree of heart damage, although further studies are needed to support this speculation.

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