A new biosensor based on the recognition of phages and the signal amplification of organic-inorganic hybrid nanoflowers for discriminating and quantitating live pathogenic bacteria in urine

2018 ◽  
Vol 258 ◽  
pp. 803-812 ◽  
Author(s):  
Yuxia Li ◽  
Guoming Xie ◽  
Juhui Qiu ◽  
Dandan Zhou ◽  
Dan Gou ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Pathogenic Bacteria ◽  
Inorganic Hybrid

Electrochemical biosensor for detection of pathogenic bacteria based on dual signal amplification of Cu3(PO4)2-mediated click chemistry and DNAzyme

The Analyst ◽  
2021 ◽  
Author(s):  
Hongguo Wei ◽  
Jiayu Wan ◽  
Shengjun Bu ◽  
Wenguang Zhang ◽  
Ma Li ◽  
...  
Keyword(s):  
Click Chemistry ◽  
Magnetic Separation ◽  
Concanavalin A ◽  
Signal Amplification ◽  
Pathogenic Bacteria ◽  
Electrochemical Biosensor ◽  
Inorganic Hybrid ◽  
Highly Sensitive ◽  
Antibody Conjugates ◽  
Dual Signal Amplification

A novel electrochemical biosensor for detecting pathogenic bacteria was designed based on specific magnetic separation and highly sensitive click chemistry. Instead of enzyme-antibody conjugates, organic-inorganic hybrid nanoflowers (Concanavalin A (Con...

scholarly journals Biosensors Coupled with Signal Amplification Technology for the Detection of Pathogenic Bacteria: A Review

Biosensors ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 190
Author(s):  
Fengchun Huang ◽  
Yingchao Zhang ◽  
Jianhan Lin ◽  
Yuanjie Liu
Keyword(s):  
Food Safety ◽  
Foodborne Pathogens ◽  
Signal Amplification ◽  
Enzyme Catalysis ◽  
Pathogenic Bacteria ◽  
Sensitive Detection ◽  
Rapid Screening ◽  
Optical Biosensors ◽  
Future Trends ◽  
Chain Reaction

Foodborne disease caused by foodborne pathogens is a very important issue in food safety. Therefore, the rapid screening and sensitive detection of foodborne pathogens is of great significance for ensuring food safety. At present, many research works have reported the application of biosensors and signal amplification technologies to achieve the rapid and sensitive detection of pathogenic bacteria. Thus, this review summarized the use of biosensors coupled with signal amplification technology for the detection of pathogenic bacteria, including (1) the development, concept, and principle of biosensors; (2) types of biosensors, such as electrochemical biosensors, optical biosensors, microfluidic biosensors, and so on; and (3) different kinds of signal amplification technologies applied in biosensors, such as enzyme catalysis, nucleic acid chain reaction, biotin-streptavidin, click chemistry, cascade reaction, nanomaterials, and so on. In addition, the challenges and future trends for pathogenic bacteria based on biosensor and signal amplification technology were also discussed and summarized.

scholarly journals Exploring Protein-Inorganic Hybrid Nanoflowers and Immune Magnetic Nanobeads to Detect Salmonella Typhimurium

Nanomaterials ◽  
2018 ◽  
Vol 8 (12) ◽  
pp. 1006 ◽  
Author(s):  
Lei Wang ◽  
Xiaoting Huo ◽  
Ruya Guo ◽  
Qiang Zhang ◽  
Jianhan Lin
Keyword(s):  
Foodborne Pathogens ◽  
Calcium Ions ◽  
Pathogenic Bacteria ◽  
Polyclonal Antibodies ◽  
Early Screening ◽  
Inorganic Hybrid ◽  
Colony Forming Units ◽  
On Line ◽  
The Mean ◽  
Magnetic Nanobeads

Early screening of pathogenic bacteria is key to preventing and controlling outbreaks of foodborne diseases. In this study, protein-inorganic hybrid nanoflowers were synthesized for signal amplification and used with a calcium ion selective electrode (Ca-ISE) to establish a new enzyme-free assay for rapid and sensitive detection of Salmonella. Calcium hydrophosphate crystals were first conjugated with polyclonal antibodies against Salmonella to synthesize immune calcium nanoflowers (CaNFs), and streptavidin modified magnetic nanobeads (MNBs) were conjugated with biotinylated monoclonal antibodies against Salmonella to form immune MNBs. After target bacteria were separated using immune MNBs to form magnetic bacteria, immune CaNFs were conjugated with magnetic bacteria to form nanoflower conjugated bacteria. Then, hydrogen chloride was used to release calcium ions from nanoflower conjugated bacteria. After magnetic separation, the supernatant was finally injected as a continuous-flow to fluidic chip with Ca-ISE for specific detection of calcium ions. The supernatant’s potential had a good linear relationship with bacteria concentration, and this assay was able to detect the S. Typhimurium cells as low as 28 colony forming units/mL within two hours. The mean recovery of target bacteria in spiked chicken samples was 95.0%. This proposed assay shows the potential for rapid, sensitive, and on-line detection of foodborne pathogens.

Multimode detection of β-glycosidase and pathogenic bacteria via cation exchange assisted signal amplification

2020 ◽  
Vol 187 (8) ◽  
Author(s):  
Xiu Wang ◽  
Wanli Chen ◽  
Haiyan Yang ◽  
Xialin Zhang ◽  
Min Deng ◽  
...  
Keyword(s):  
Cation Exchange ◽  
Signal Amplification ◽  
Pathogenic Bacteria

Probiotic Displaces Pathogenic Bacteria

2006 ◽  
Vol 37 (7) ◽  
pp. 48
Author(s):  
ERIK GOLDMAN
Keyword(s):  
Pathogenic Bacteria

Iron and Malaria: Absorption, Efficacy and Safety

2010 ◽  
Vol 80 (45) ◽  
pp. 279-292 ◽  
Author(s):  
Richard Hurrell
Keyword(s):  
Pathogenic Bacteria ◽  
Intestinal Barrier ◽  
Systemic Circulation ◽  
Malarial Parasite ◽  
Asymptomatic Malaria ◽  
Iron Supplements ◽  
Fortified Food ◽  
Care Giving ◽  
Single Meal ◽  
Women And Children

Febrile malaria and asymptomatic malaria parasitemia substantially decrease iron absorption in single-meal, stable isotope studies in women and children, but to date there is no evidence of decreased efficacy of iron-fortified foods in malaria-endemic regions. Without inadequate malarial surveillance or health care, giving iron supplements to children in areas of high transmission could increase morbidity and mortality. The most likely explanation is the appearance of non-transferrin-bound iron (NTBI) in the plasma. NTBI forms when the rate of iron influx into the plasma exceeds the rate of iron binding to transferrin. Two studies in women have reported substantially increased NTBI with the ingestion of iron supplements. Our studies confirm this, but found no significant increase in NTBI on consumption of iron-fortified food. It seems likely that the malarial parasite in hepatocytes can utilize NTBI, but it cannot do so in infected erythrocytes. NTBI however may increase the sequestration of parasite-infected erythrocytes in capillaries. Bacteremia is common in children with severe malaria and sequestration in villi capillaries could lead to a breaching of the intestinal barrier, allowing the passage of pathogenic bacteria into the systemic circulation. This is especially important as frequent high iron doses increase the number of pathogens in the intestine at the expense of the barrier bacteria.

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