53. Transplantation of Goat Bone Marrow-Derived Stromal Cells to a Degenerative Intervertebral Disc in a Goat Disc-Injury Model

2009 ◽  
Vol 9 (10) ◽  
pp. 27S
Author(s):  
Yejia Zhang ◽  
Susan Drapeau ◽  
Howard An ◽  
Eugene Thonar ◽  
D. Greg Anderson
Keyword(s):  
Bone Marrow ◽  
Intervertebral Disc ◽  
Stromal Cells ◽  
Injury Model ◽  
Disc Injury

scholarly journals Transplantation of Goat Bone Marrow Stromal Cells to the Degenerating Intervertebral Discin a Goat Disc Injury Model

Spine ◽  
2011 ◽  
Vol 36 (5) ◽  
pp. 372-377 ◽  
Author(s):  
Yejia Zhang ◽  
Susan Drapeau ◽  
S. An Howard ◽  
Eugene J. M. A. Thonar ◽  
D. Greg Anderson
Keyword(s):  
Bone Marrow ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Marrow Stromal Cells ◽  
Injury Model ◽  
Disc Injury

Cryopreserved intervertebral disc with injected bone marrow–derived stromal cells: a feasibility study using organ culture

2010 ◽  
Vol 10 (6) ◽  
pp. 486-496 ◽  
Author(s):  
Samantha C.W. Chan ◽  
Benjamin Gantenbein-Ritter ◽  
Victor Y.L. Leung ◽  
Danny Chan ◽  
Kenneth M.C. Cheung ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Intervertebral Disc ◽  
Organ Culture ◽  
Feasibility Study ◽  
Stromal Cells

Prevention and Treatment of Intervertebral Disc Degeneration with Bone Marrow Derived Stem (Stromal) Cells—An in Vivo Study in Sheep

2014 ◽  
Vol 4 (1_suppl) ◽  
pp. s-0034-1376572-s-0034-1376572
Author(s):  
J. Melrose ◽  
C. Shu ◽  
A. Dart ◽  
E. C. Clarke ◽  
J. Martin ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Intervertebral Disc ◽  
Disc Degeneration ◽  
Stromal Cells ◽  
Intervertebral Disc Degeneration ◽  
In Vivo Study ◽  
Prevention And Treatment

scholarly journals Radiological, Histological and Biochemical Characterization of a Novel Ovine Lumbar Intervertebral Disc Injury Model

2016 ◽  
Vol 6 (1_suppl) ◽  
pp. s-0036-1582625-s-0036-1582625
Author(s):  
Christopher Daly ◽  
Tony Goldschlager ◽  
Peter Ghosh ◽  
Graham Jenkin ◽  
Justin Cooper-White ◽  
...  
Keyword(s):  
Intervertebral Disc ◽  
Biochemical Characterization ◽  
Lumbar Intervertebral Disc ◽  
Injury Model ◽  
Disc Injury

In Vivo Fluorescence Tracking of Bone Marrow Stromal Cells Transplanted into a Pneumatic Injury Model of Rat Spinal Cord

2005 ◽  
Vol 22 (8) ◽  
pp. 907-918 ◽  
Author(s):  
Shunsuke Yano ◽  
Satoshi Kuroda ◽  
Jang-Bo Lee ◽  
Hideo Shichinohe ◽  
Toshitaka Seki ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Bone Marrow ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Marrow Stromal Cells ◽  
Rat Spinal Cord ◽  
In Vivo Fluorescence ◽  
Injury Model

scholarly journals Bleomycin induces fibrotic transformation of bone marrow stromal cells to treat height loss of intervertebral disc through the TGFβR1/Smad2/3 pathway

2020 ◽  
Author(s):  
Xiao Yang ◽  
zhiqian chen ◽  
Chen Chen ◽  
Chen Han ◽  
Yifan Zhou ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Intervertebral Disc ◽  
Signaling Pathway ◽  
Histological Examination ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Transforming Growth Factor ◽  
Marrow Stromal Cells ◽  
Cell Counting ◽  
Height Loss

Abstract Background: Lower back pain is often accredited to loss of intervertebral disc (IVD) height and compromised spine stability as a result of intervertebral disc degeneration (IVDD). We aim to locally use Bleomycin to induce the fibrotic transformation of bone marrow stromal cells (BMSCs) as a means to induce reparative fibrosis to slow down the height loss. Methods: IVD from patients were gathered for histological examination. The expression of transforming growth factor beta 1 (TGF-β) signaling pathway was determined by qPCR and western blotting. Nucleus pulposus (NP) cells, annulus fibrosus (AF) cells and the Rats’ bone marrow stromal cells (BMSC)were cultured and their responsiveness to Bleomycin was evaluated by Cell Counting Kit-8, comet assay, transwell migration and wound healing assays. Rat IVDD models were created by puncture, rescued by Bleomycin injection and the effectiveness was evaluated by images (X-ray and MRI) and atomic force microscope. Results: Histological examination showed increased levels of pro-fibrotic markers in IVDD tissues from patients. AF cells and BMSC cells was induced to adopt to a pro-fibrotic phenotype with increased expression fibrotic markers Col1a1, Col3a1, FSP1. The pro-fibrotic effect of Bleomycin on AF cells and BMSCs was in part due to the activation of the TGFβ-TGFβR1-SMAD2/3 signaling pathway. Pharmacological inhibition or gene knock-down of TGFβR1 could mitigate the pro-fibrotic effects. Conclusion: Locally injection of Bleomycin in rats’ IVD induced rapid fibrosis and maintained its height through TGFβ-TGFβR1-SMAD2/3 signaling pathway.

scholarly journals Histological Features of the Degenerating Intervertebral Disc in a Goat Disc-Injury Model

Spine ◽  
2011 ◽  
Vol 36 (19) ◽  
pp. 1519-1527 ◽  
Author(s):  
Yejia Zhang ◽  
Susan Drapeau ◽  
Howard S. An ◽  
Dessislava Markova ◽  
Brett A. Lenart ◽  
...  
Keyword(s):  
Intervertebral Disc ◽  
Histological Features ◽  
Injury Model ◽  
Disc Injury

scholarly journals TNFAIP8 family gene expressions in the mouse tail intervertebral disc injury model

JOR Spine ◽  
2020 ◽  
Vol 3 (2) ◽  
Author(s):  
Zuozhen Tian ◽  
Frances S. Shofer ◽  
Lutian Yao ◽  
Honghong Sun ◽  
Hongtao Zhang ◽  
...  
Keyword(s):  
Intervertebral Disc ◽  
Gene Expressions ◽  
Injury Model ◽  
Disc Injury ◽  
Family Gene
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