ELEFTHERIOS H. DROSINOS
◽
MARIOS MATARAGAS
◽
SLAVICA VESKOVIĆ-MORAČANIN
◽
JUDIT GASPARIK-REICHARDT
◽
MIRZA HADŽIOSMANOVIĆ
◽
...
Listeria monocytogenes NCTC10527 was examined with respect to its nonthermal inactivation kinetics in fermented sausages from four European countries: Serbia-Montenegro, Hungary, Croatia, and Bosnia-Herzegovina. The goal was to quantify the effect of fermentation and ripening conditions on L. monocytogenes with the simultaneous presence or absence of bacteriocin-producing lactic acid bacteria (i.e., Lactobacillus sakei). Different models were used to fit the experimental data and to calculate the kinetic parameters. The best model was chosen based on statistical comparisons. The Baranyi model was selected because it fitted the data better in most (73%) of the cases. The results from the challenge experiments and the subsequent statistical analysis indicated that relative to the control condition the addition of L. sakei strains reduced the time required for a 4-log reduction of L. monocytogenes (t4D). In contrast, the addition of the bacteriocins mesenterocin Y and sakacin P decreased the t4D values for only the Serbian product. A case study for risk assessment also was conducted. The data of initial population and t4D collected from all countries were described by a single distribution function. Storage temperature, packaging method, pH, and water activity of the final products were used to calculate the inactivation of L. monocytogenes that might occur during storage of the final product (U.S. Department of Agriculture Pathogen Modeling Program version 7.0). Simulation results indicated that the addition of L. sakei strains significantly decreased the simulated L. monocytogenes concentration of ready-to-eat fermented sausages at the time of consumption.
Detection and molecular characterization of butyrate-producing genes in probiotic lactic acid bacteria for use in livestock
