Automated flow-based anion-exchange method for high-throughput isolation and real-time monitoring of RuBisCO in plant extracts

Talanta ◽  
2011 ◽  
Vol 84 (5) ◽  
pp. 1259-1266 ◽  
Author(s):  
Ruth Suárez ◽  
Manuel Miró ◽  
Víctor Cerdà ◽  
Juan Alejandro Perdomo ◽  
Jeroni Galmés
Keyword(s):  
Real Time ◽  
Anion Exchange ◽  
High Throughput ◽  
Plant Extracts ◽  
Real Time Monitoring ◽  
Exchange Method

High-Throughput and Real-Time Monitoring of Single-Cell Extracellular pH Based on Polyaniline Microarrays

2021 ◽  
Author(s):  
Lihui Yang ◽  
Xiaobo Liu ◽  
Bing Yin ◽  
Xunxun Deng ◽  
Xiaotong Lin ◽  
...  
Keyword(s):  
Single Cell ◽  
Real Time ◽  
High Throughput ◽  
Extracellular Ph ◽  
Real Time Monitoring

Real-Time Monitoring of Luciferase-Tagged Cronobacter sakazakii in Reconstituted Infant Milk Formula

2011 ◽  
Vol 74 (4) ◽  
pp. 573-579 ◽  
Author(s):  
RUTH MORRISSEY ◽  
MÁIRE BEGLEY ◽  
SATORU OSHIMA ◽  
MARY REA ◽  
R. PAUL ROSS ◽  
...  
Keyword(s):  
Real Time ◽  
High Throughput ◽  
Skim Milk ◽  
Luria Bertani ◽  
Milk Formula ◽  
Cronobacter Sakazakii ◽  
Real Time Monitoring ◽  
Luria Bertani Broth ◽  
Infant Milk Formula

The aim of this study was to examine the potential of using a lux-tagged Cronobacter sakazakii strain to monitor growth of the bacterium in various liquids. C. sakazakii was transformed with plasmid p16Slux, and integration of the plasmid at the desired site on the chromosome was confirmed by PCR. The growth of the lux-tagged strain was similar to that of the non–lux-tagged strain, and the integrated plasmid was stable when cells were cultured in the absence of antibiotic. Growth of the lux-tagged strain was monitored in real time in Luria-Bertani broth, skim milk, and infant milk formula by using both the Luminoskan luminometer and the Xenogen IVIS imager. Bioluminescence could be detected when the lux-tagged strain was cocultured with other bacteria. The effect of monocaprylin and nisin on the growth of C. sakazakii in milk was monitored by measuring bioluminescence. In conclusion, growth of a lux-tagged C. sakazakii can be monitored in real time in both clear and opaque liquids by measuring bioluminescence. lux-tagged C. sakazakii strains could be potentially used in high-throughput assays to monitor the effects of various infant milk formula compositions on growth of the bacterium.

scholarly journals A semi high-throughput method for real-time monitoring of curli producing Salmonella biofilms on air-solid interfaces

Biofilm ◽  
2021 ◽  
pp. 100060
Author(s):  
Ferdinand X. Choong ◽  
Smilla Huzell ◽  
Ming Rosenberg ◽  
Johannes A. Eckert ◽  
Madhu Nagaraj ◽  
...  
Keyword(s):  
Real Time ◽  
High Throughput ◽  
Real Time Monitoring ◽  
High Throughput Method

In-situ nanoelectrospray for high-throughput screening of enzymes and real-time monitoring of reactions

2016 ◽  
Vol 902 ◽  
pp. 135-141 ◽  
Author(s):  
Yuhan Yang ◽  
Feifei Han ◽  
Jin Ouyang ◽  
Yunling Zhao ◽  
Juan Han ◽  
...  
Keyword(s):  
Real Time ◽  
High Throughput ◽  
High Throughput Screening ◽  
Real Time Monitoring

High-Throughput, Real-Time Monitoring of the Self-Assembly of DNA Nanostructures by FRET Spectroscopy

2008 ◽  
Vol 47 (11) ◽  
pp. 2135-2137 ◽  
Author(s):  
Barbara Saccà ◽  
Rebecca Meyer ◽  
Udo Feldkamp ◽  
Hendrik Schroeder ◽  
Christof M. Niemeyer
Keyword(s):  
Real Time ◽  
High Throughput ◽  
Self Assembly ◽  
The Self ◽  
Dna Nanostructures ◽  
Real Time Monitoring
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