Application of a cholesterol stationary phase in the analysis of phosphorothioate oligonucleotides by means of ion pair chromatography coupled with tandem mass spectrometry

Talanta ◽  
2016 ◽  
Vol 154 ◽  
pp. 270-277 ◽  
Author(s):  
Sylwia Studzińska ◽  
Katarzyna Krzemińska ◽  
Michał Szumski ◽  
Bogusław Buszewski
Keyword(s):  
Mass Spectrometry ◽  
Tandem Mass Spectrometry ◽  
Stationary Phase ◽  
Ion Pair ◽  
Tandem Mass ◽  
Ion Pair Chromatography

Liquid–liquid microextraction of glyphosate, glufosinate and aminomethylphosphonic acid for the analysis of agricultural samples by liquid chromatography

2020 ◽  
Vol 12 (15) ◽  
pp. 2039-2045
Author(s):  
Javier Marín ◽  
Natalia Campillo ◽  
Manuel Hernández-Córdoba ◽  
Isabel Garrido ◽  
José Fenoll ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Ion Pair ◽  
Tandem Mass ◽  
Aminomethylphosphonic Acid ◽  
Sensitive Determination ◽  
Liquid Microextraction

An ion-pair liquid–liquid microextraction procedure followed by LC tandem mass spectrometry allows a reliable and sensitive determination glyphosate, glufosinate y aminomethylphosphonic acid in agricultural samples.

Development of an Ion-Pair Reverse-Phase Liquid Chromatographic/Tandem Mass Spectrometry Method for the Determination of an 18-Mer Phosphorothioate Oligonucleotide in Mouse Liver Tissue

2005 ◽  
Vol 11 (2) ◽  
pp. 209-215 ◽  
Author(s):  
Anthony T. Murphy ◽  
Patricia Brown-Augsburger ◽  
Rosie Z. Yu ◽  
Richard S. Geary ◽  
Stefan Thibodeaux ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Tandem Mass Spectrometry ◽  
Liver Tissue ◽  
Mouse Liver ◽  
Ion Pair ◽  
Tandem Mass ◽  
Phosphorothioate Oligonucleotide ◽  
Reverse Phase ◽  
Discovery Research

A quantitative method for the determination of a partially modified, 2′-ribose alkoxy 18-mer phosphorothioate oligonucleotide in liver tissue has been developed. A liquid:liquid extraction, ion-pair reverse phase chromatographic separation and tandem mass spectrometry were used to achieve a quantitation range of 125 to 10,000 ng g−1 mouse liver tissue. A total cycle time of 5 min was obtained while maintaining separation of three potential impurities. Separations were performed using a Discovery RP-Amide C16, 100 × 2 mm column packed with 5 μm particles. The separation was facilitated by the use of triethylamine (TEA) and hexafluoroisopropanol (HFIP) as ion-pair agents. The method has subsequently been used for the determination of other phosphorothioate oligonucleotides in support of discovery research.

Analysis of carvedilol enantiomers in human plasma using chiral stationary phase column and liquid chromatography with tandem mass spectrometry

Chirality ◽  
2012 ◽  
Vol 24 (3) ◽  
pp. 209-214 ◽  
Author(s):  
Josiane Cristófani Poggi ◽  
Flávia Garcez Da Silva ◽  
Eduardo Barbosa Coelho ◽  
Maria Paula Marques ◽  
Carlo Bertucci ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Stationary Phase ◽  
Human Plasma ◽  
Chiral Stationary Phase ◽  
Tandem Mass ◽  
Phase Column
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