A target-triggered catalytic hairpin assembly with a G-quadruplex specific fluorescent binder, NMM, is employed to develop a novel and sensitive RNase H activity assay.
In this study, a point-of-care testing (POCT) method for the detection of ricin B-chain (RTB) using a commercial peroxide test strip (PTS) combined with signal amplification via glucose oxidase-loaded liposome and catalytic hairpin assembly (CHA) was reported.
Branched catalytic hairpin assembly is proposed for dynamic self-assembly of DNA dendrimers for signal amplification and in situ imaging of microRNAs in live cells with high sensitivity and selectivity.