Catalytic hairpin assembly mediated liposome-encoded magnetic beads for signal amplification of peroxide test strip based point-of-care testing of ricin

2020 ◽  
Vol 56 (90) ◽  
pp. 14091-14094
Author(s):  
Qi Chao Ye ◽  
Chen Men ◽  
Yuan Fang Li ◽  
Jia Jun Liu ◽  
Cheng Zhi Huang ◽  
...  
Keyword(s):  
Glucose Oxidase ◽  
Signal Amplification ◽  
Magnetic Beads ◽  
Point Of Care ◽  
Test Strip ◽  
Point Of Care Testing ◽  
Catalytic Hairpin Assembly

In this study, a point-of-care testing (POCT) method for the detection of ricin B-chain (RTB) using a commercial peroxide test strip (PTS) combined with signal amplification via glucose oxidase-loaded liposome and catalytic hairpin assembly (CHA) was reported.

scholarly journals Disposable Voltammetric Immunosensors Integrated with Microfluidic Platforms for Biomedical, Agricultural and Food Analyses: A Review

Sensors ◽  
2018 ◽  
Vol 18 (12) ◽  
pp. 4124 ◽  
Author(s):  
Fabiana Felix ◽  
Alexandre Baccaro ◽  
Lúcio Angnes
Keyword(s):  
Quality Management ◽  
Signal Amplification ◽  
Screen Printing ◽  
Point Of Care ◽  
Microfluidic Devices ◽  
Strong Interactions ◽  
Point Of Care Testing ◽  
Microfluidic Platforms ◽  
Broad Variety ◽  
Interesting Alternative

Disposable immunosensors are analytical devices used for the quantification of a broad variety of analytes in different areas such as clinical, environmental, agricultural and food quality management. They detect the analytes by means of the strong interactions between antibodies and antigens, which provide concentration-dependent signals. For the herein highlighted voltammetric immunosensors, the analytical measurements are due to changes in the electrical signals on the surface of the transducers. The possibility of using disposable and miniaturized immunoassays is a very interesting alternative for voltammetric analyses, mainly, when associated with screen-printing technologies (screen-printed electrodes, SPEs), and microfluidic platforms. The aim of this paper is to discuss a carefully selected literature about different examples of SPEs-based immunosensors associated with microfluidic technologies for diseases, food, agricultural and environmental analysis. Technological aspects of the development of the voltammetric immunoassays such as the signal amplification, construction of paper-based microfluidic platforms and the utilization of microfluidic devices for point-of-care testing will be presented as well.

Analytical Interferences in Point-of-Care Testing Glucometers by Icodextrin and its Metabolites: An Overview

2009 ◽  
Vol 29 (4) ◽  
pp. 377-383 ◽  
Author(s):  
Katelijne M.J. Floré ◽  
Joris R. Delanghe
Keyword(s):  
Glucose Oxidase ◽  
Point Of Care ◽  
Glucose Dehydrogenase ◽  
Systemic Circulation ◽  
Test Method ◽  
High Risk Population ◽  
Lower Sensitivity ◽  
Point Of Care Testing ◽  
Glucose Levels ◽  
Blood Glucose Levels

Current point-of-care testing (POCT) glucometers are based on various test principles. Two major method groups dominate the market: glucose oxidase-based systems and glucose dehydrogenase-based systems using pyrroloquinoline quinone (GDH-PQQ) as a cofactor. The GDH-PQQ-based glucometers are replacing the older glucose oxidase-based systems because of their lower sensitivity for oxygen. On the other hand, the GDH-PQQ test method results in falsely elevated blood glucose levels in peritoneal dialysis patients receiving solutions containing icodextrin ( e.g., Extraneal; Baxter, Brussels, Belgium). Icodextrin is metabolized in the systemic circulation into different glucose polymers, but mainly maltose, which interferes with the GDH-PQQ-based method. Clinicians should be aware of this analytical interference. The POCT glucometers based on the GDH-PQQ method should preferably not be used in this high-risk population and POCT glucose results inconsistent with clinical suspicion of hypoglycemic coma should be retested with another testing system.

scholarly journals A low-cost smartphone-based platform for highly sensitive point-of-care testing with persistent luminescent phosphors

Lab on a Chip ◽  
2017 ◽  
Vol 17 (6) ◽  
pp. 1051-1059 ◽  
Author(s):  
Andrew S. Paterson ◽  
Balakrishnan Raja ◽  
Vinay Mandadi ◽  
Blane Townsend ◽  
Miles Lee ◽  
...  
Keyword(s):  
Point Of Care ◽  
Low Cost ◽  
Test Strip ◽  
Lateral Flow ◽  
Point Of Care Testing ◽  
Flow Test ◽  
Highly Sensitive ◽  
Lateral Flow Test ◽  
Gated Imaging

Time-gated imaging on a smartphone of a lateral flow test strip run with persistent luminescent nanophosphors.

Pressure/colorimetric dual-readout immunochromatographic test strip for point-of-care testing of aflatoxin B1

Talanta ◽  
2021 ◽  
Vol 227 ◽  
pp. 122203
Author(s):  
Shan Jiang ◽  
Lvxia Zhang ◽  
Jizhou Li ◽  
Hui Ouyang ◽  
Zhifeng Fu
Keyword(s):  
Aflatoxin B1 ◽  
Point Of Care ◽  
Test Strip ◽  
Immunochromatographic Test ◽  
Point Of Care Testing

Bioorthogonal Reaction-Mediated ELISA Using Peroxide Test Strip as Signal Readout for Point-of-Care Testing

2017 ◽  
Vol 89 (11) ◽  
pp. 6113-6119 ◽  
Author(s):  
Bei Ran ◽  
Yunlei Xianyu ◽  
Mingling Dong ◽  
Yiping Chen ◽  
Zhiyong Qian ◽  
...  
Keyword(s):  
Point Of Care ◽  
Test Strip ◽  
Point Of Care Testing ◽  
Bioorthogonal Reaction ◽  
Signal Readout

Clinical laboratories have a critical role in test strip lot management in glucose point-of-care testing

2016 ◽  
Vol 54 (5) ◽  
Author(s):  
Mattias Hofmans ◽  
Matthijs Oyaert ◽  
Patricia De Schrijver ◽  
Frank Nobels ◽  
Lieve Van Hoovels
Keyword(s):  
Point Of Care ◽  
Critical Role ◽  
Test Strip ◽  
Point Of Care Testing ◽  
Clinical Laboratories

scholarly journals An ultraportable and versatile point-of-care DNA testing platform

2020 ◽  
Vol 6 (17) ◽  
pp. eaaz7445 ◽  
Author(s):  
Huan Xu ◽  
Anyue Xia ◽  
Dandan Wang ◽  
Yiheng Zhang ◽  
Shaoli Deng ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Point Of Care ◽  
Clinical Diagnostics ◽  
Dna Testing ◽  
Point Of Care Testing ◽  
Resource Limited Settings ◽  
Single Base ◽  
Signal Detector ◽  
Resource Limited ◽  
Different Types

Point-of-care testing (POCT) has broad applications in resource-limited settings. Here, a POCT platform termed POCKET (point-of-care kit for the entire test) is demonstrated that is ultraportable and versatile for analyzing multiple types of DNA in different fields in a sample-to-answer manner. The POCKET is less than 100 g and smaller than 25 cm in length. The kit consists of an integrated chip (i-chip) and a foldable box (f-box). The i-chip integrates the sample preparation with a previously unidentified, triple signal amplification. The f-box uses a smartphone as a heater, a signal detector, and a result readout. We detected different types of DNA from clinics to environment to food to agriculture. The detection is sensitive (<103 copies/ml), specific (single-base differentiation), speedy (<2 hours), and stable (>10 weeks shelf life). This inexpensive, ultraportable POCKET platform may become a versatile sample-to-answer platform for clinical diagnostics, food safety, agricultural protection, and environmental monitoring.

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