Differential scanning calorimetry of human blood serum exposed in vitro to X-ray radiation

2019 ◽  
Vol 680 ◽  
pp. 178358 ◽  
Author(s):  
Agnieszka Kiełboń ◽  
Anna Michnik ◽  
Kinga Polaczek Grelik ◽  
Klaudia Duch ◽  
Ewa Sadowska-Krępa
Keyword(s):  
Differential Scanning Calorimetry ◽  
Blood Serum ◽  
Human Blood ◽  
Human Blood Serum ◽  
Scanning Calorimetry ◽  
X Ray

Measuring human blood serum with chip based fast liquid differential scanning calorimetry

2016 ◽  
Vol 639 ◽  
pp. 76-83 ◽  
Author(s):  
R. Splinter ◽  
A.W. van Herwaarden ◽  
S. Pastorekova ◽  
T.C. Linders ◽  
T. Korse ◽  
...  
Keyword(s):  
Differential Scanning Calorimetry ◽  
Blood Serum ◽  
Human Blood ◽  
Human Blood Serum ◽  
Scanning Calorimetry

The preparation, characterization, structure and dissolution analysis of apremilast solvatomorphs

2017 ◽  
Vol 73 (4) ◽  
pp. 305-313 ◽  
Author(s):  
Yun-Deng Wu ◽  
Xiao-Lei Zhang ◽  
Xiao-Hong Liu ◽  
Jian Xu ◽  
Mei Zhang ◽  
...  
Keyword(s):  
Differential Scanning Calorimetry ◽  
Hydrogen Bonding ◽  
Psoriatic Arthritis ◽  
X Ray Diffraction ◽  
Scanning Calorimetry ◽  
Dissolution Rates ◽  
X Ray ◽  
Similarity Factor ◽  
Hydrogen Bonding Interactions

Apremilast (AP) {systematic name: (S)-2-[1-(3-ethoxy-4-methoxyphenyl)-2-(methylsulfonyl)ethyl]-4-acetamidoisoindoline-1,3-dione} is an inhibitor of phosphodieasterase-4 (PDE4) and is indicated for the treatment of adult patients with active psoriatic arthritis. The ability of AP to form solvates has been investigated and three solvatomorphs of AP, namely, the AP ethyl acetate hemisolvate, C22H24N2O7S·0.5C4H8O2, the AP toluene hemisolvate, C22H24N2O7S·0.5C7H8, and the AP dichloromethane monosolvate, C22H24N2O7S·CH2Cl2, were obtained. The three AP solvatomorphs were characterized by X-ray powder diffraction, thermogravimetric analysis and differential scanning calorimetry. Single-crystal X-ray diffraction was used to analyze the structures, crystal symmetry, packing modes, stoichiometry and hydrogen-bonding interactions of the solvatomorphs. In addition, dissolution analyses were performed to study the dissolution rates of different AP solvatomorph tablets in vitro and to make comparisons with commercial apremilast tablets (produced by Celgene); all three solvatomorphs showed similar dissolution rates and similar values of the similarity factor f2 in a comparison of their dissolution profiles.

scholarly journals Identification of hepatitis C in human blood serum by near-infrared Raman spectroscopy

2008 ◽  
Vol 22 (5) ◽  
pp. 387-395 ◽  
Author(s):  
Jamil Saade ◽  
Marcos Tadeu Tavares Pacheco ◽  
Maira Regina Rodrigues ◽  
Landulfo Silveira Jr.
Keyword(s):  
Raman Spectroscopy ◽  
Hepatitis C ◽  
Blood Serum ◽  
Human Blood ◽  
Near Infrared ◽  
Viral Infections ◽  
Human Blood Serum ◽  
Biochemical Alterations ◽  
Viral Hepatitis C

Hepatitis C has become one of the higher health problems around the world. Near-infrared Raman spectroscopy had been used to spectrally differentiate among health human blood serum from the one with hepatitis C contaminationin vitro. In this study a Raman spectrometer with 80 mW, 830 nm excitation, liquid-nitrogen cooled CCD and imaging spectrograph were used to collect Raman scattering from 24 blood samples (14 healthy and 10 diseased) with collection time of 120 s. It has been used an algorithm based on the Principal Components Analysis (PCA) for main spectral features identification and Mahalanobis distance for blood spectrum classification depending on the serology. It was observed that the highest spectral differences between the two types of human blood serum were found in 1002, 1169, 1262 and 1348 cm−1Raman bands. The spectral analysis using multivariate statistics presented good results when compared to classical diagnosis for viral hepatitis C, showing that Raman spectroscopy can classify human blood serum spectrum in one of the two categories by identifying biochemical alterations that occur in the presence of viral infections.

scholarly journals PARTIAL AMORPHIZATION OF POORLY-SOLUBLE SIMVASTATIN USP USING MEDIA MILLING IN SYNERGISM WITH SPRAY-DRYING

2020 ◽  
pp. 114-121
Author(s):  
HARITA R. DESAI ◽  
ARCHANA B. RAJADHYAX ◽  
PURNIMA D. AMIN
Keyword(s):  
Differential Scanning Calorimetry ◽  
Spray Drying ◽  
Scanning Calorimetry ◽  
Intrinsic Dissolution ◽  
X Ray ◽  
Media Milling ◽  
Rate Testing ◽  
Poorly Soluble ◽  
Spray Dried

Objective: The objective of the current study was to explore top down methods of size reduction like high speed homogenisation and media milling in synergism with spray drying in amorphization and solubility enhancement of BCS Class II antilipidemic drug Simvastatin USP. Methods: Spray-dried micronized simvastatin USP was formulated by homogenisation and media milling of drug suspension in optimized stabilizer solution. Stabilizer combination, duration of homogenisation and ball milling and drug: stabilizer ratio was optimized. The obtained dispersion was transformed into solid powder using spray drying. The obtained Spray-dried micronized Simvastatin USP was evaluated for visual morphology, Infrared spectroscopy, Differential scanning calorimetry, in vitro drug release studies, X-Ray diffractometry, Scanning electron microscopy, contact angle measurement, solubility studies, dispersibility studies and intrinsic dissolution rate testing. Results: Spray-dried micronized simvastatin USP was found to show amorphization of crystalline Simvastatin USP as confirmed by the absence of drug peak in Differential scanning calorimetry and lowered signal intensity in X-Ray diffraction studies. Spray-dried micronized Simvastatin USP was found to show enhanced drug hydrophilicity and solubility as confirmed by lowering in contact angle and increase in solubility and ease of dispersibility observations. In vitro dissolution testing and intrinsic dissolution rate testing were found to show an increase in drug release from 11% to 79% and 4 mg min-1 cm-2 to 17 mg min-1 cm-2 for drug and Spray-dried micronized Simvastatin USP respectively. Conclusion: Media milling in synergism with spray-drying was found to be a prospective solubility enhancement technique for poorly-soluble Simvastatin USP.

LIFE IN ZERO MAGNETIC FIELD. III. EFFECT ON ZINC AND COPPER IN HUMAN BLOOD SERUM DURING IN VITRO AGING

2001 ◽  
Vol 20 (2) ◽  
pp. 127-139 ◽  
Author(s):  
Lorelai I. Ciortea ◽  
V. V. Morariu ◽  
Alina Todoran ◽  
S. Popescu
Keyword(s):  
Magnetic Field ◽  
Blood Serum ◽  
Human Blood ◽  
Human Blood Serum ◽  
Zero Magnetic Field ◽  
In Vitro Aging

scholarly journals Comparison of human blood serum DSC profiles in aqueous and PBS buffer solutions

2021 ◽  
Author(s):  
Anna Michnik ◽  
Agnieszka Kiełboń ◽  
Klaudia Duch ◽  
Ewa Sadowska-Krępa ◽  
Ilona Pokora
Keyword(s):  
Blood Serum ◽  
Human Blood ◽  
Biological Fluids ◽  
Water Solution ◽  
Medical Diagnostics ◽  
Human Blood Serum ◽  
Scanning Calorimetry ◽  
Dsc Method ◽  
Saline Solutions ◽  
Research Stage

AbstractThe results of studies of physiological fluids by differential scanning calorimetry (DSC) for the purpose of diagnosis and monitoring of diseases are promising. Before the DSC method is accepted in medical diagnostics, it is worth experimenting with various environmental conditions at the research stage. Among other things, it is important to choose an appropriate solvent to dilute the tested biological fluids. In this work, human blood sera DSC profiles in aqueous and PBS (phosphate-buffered saline) solutions have been compared. Visibility of haptoglobin in the DSC profile of human blood serum is much better in water solution. In addition, contributions from albumin and haptoglobin are well separated in contrast to the PBS serum solutions. The peak or shoulder at about 83 °C which represents contributions from the CH3 domain of immunoglobulin IgG1 and/or transferrin is more clearly visible in PBS solution. The results show that the type of solvent is essential when interpreting the serum DSC profile.

Sign in / Sign up

Export Citation Format

Share Document