The evaluation of corpus luteum blood flow using color-flow Doppler ultrasound for early pregnancy diagnosis in bovine embryo recipients

2009 ◽  
Vol 71 (4) ◽  
pp. 707-715 ◽  
Author(s):  
M.D. Utt ◽  
G.L. Johnson ◽  
W.E. Beal
Keyword(s):  
Blood Flow ◽  
Corpus Luteum ◽  
Doppler Ultrasound ◽  
Early Pregnancy ◽  
Bovine Embryo ◽  
Color Flow ◽  
Pregnancy Diagnosis

100 Relationship between corpus luteum blood flow evaluated via colour Doppler ultrasound and pregnancy rate in bovine embryo transfer recipients

2020 ◽  
Vol 32 (2) ◽  
pp. 176
Author(s):  
M. K. Sermersheim ◽  
B. R. Lindsey ◽  
L. M. Naves ◽  
M. Rubessa ◽  
M. B. Wheeler
Keyword(s):  
Blood Flow ◽  
Medical Imaging ◽  
Corpus Luteum ◽  
Pregnancy Rate ◽  
Embryo Transfer ◽  
Doppler Ultrasound ◽  
Doppler Imaging ◽  
Bovine Embryo ◽  
Colour Doppler ◽  
Colour Doppler Ultrasound

The advent of colour Doppler ultrasound has allowed evaluation of blood flow to bodily tissues. This novel technology is being tested as a tool to improve efficiency in a variety of livestock reproduction programs. The objective of the present study was to evaluate the relationship between blood flow to the ovarian corpus luteum (CL), imaged via colour Doppler ultrasound, and pregnancy rate in crossbred dairy recipients for embryo transfer (ET). Oestrous cycles of Bos indicus×Bos taurus dairy heifers (n=90, 16-24 months of age) were synchronized for embryo transfer on Days 7-8. Immediately before ET, heifers were palpated for the presence of ovarian CL and CL papillae. Presence of CL, CL papillae, and CL lacunae were confirmed via transrectal B-mode ultrasound (Ibex EVO I, E. I. Medical Imaging). Transrectal colour Doppler ultrasound (Ibex EVO I, E. I. Medical Imaging) was used to evaluate blood flow to the CL. Invitro-produced (IVP) blastocysts (qualities I and II) were transferred to the uterine horn ipsilateral to the CL. All palpation, imaging, and embryo transfer was performed by a single technician. Pregnancy was determined via transrectal ultrasound 24-32 days post-embryo transfer (31-39 days of embryo age). Blood flow to the CL was split into three categories: high, median, and low. Presence or absence of two additional CL structures, CL papillae and lacunae, were recorded. Fisher's exact test was used to evaluate data. Statistical differences were considered significant at P<0.05. The overall pregnancy rate was determined to be 40%, 36 out of 90. Pregnancy rates were similar in high (33.9%, 20 of 59), median (59.09%, 13 of 22), and low (33.33%, 3 of 9) blood flow categories. Consistent with previous studies, absence of CL papillae and presence of CL lacunae at the time of ET did not affect pregnancy rate. Doppler blood flow, papillae, and lacunae were also evaluated together in each possible combination. Only one outlier was present; all other differences were not significant. The highest pregnancy rate (66.67%, 10 of 15) was observed in recipients with CLs with median Doppler blood flow, a palpable CL papillae, and no CL lacunae. A larger sample size is required to determine the accuracy of this measurement. In conclusion, recipients with high to low CL blood flow are suitable for IVP embryo transfer programs. Alone, colour Doppler imaging of the CL does not predict pregnancy rate.

scholarly journals The luteal blood flow, area and pixel intensity of corpus luteum, levels of progesterone in pregnant and nonpregnant mares in the period of 16 days after ovulation

2013 ◽  
Vol 58 (No. 11) ◽  
pp. 512-519 ◽  
Author(s):  
J. Šichtař ◽  
R. Rajmon ◽  
K. Hošková ◽  
D. Řehák ◽  
L. Vostrý ◽  
...  
Keyword(s):  
Blood Flow ◽  
Corpus Luteum ◽  
Early Pregnancy ◽  
Repeated Measures ◽  
Power Doppler ◽  
Mixed Linear Model ◽  
Developmental Differences ◽  
Flow Area ◽  
Pixel Intensity ◽  
Pregnancy Diagnosis

The objective of the present study was to examine if luteal blood flow (LBF) monitoring could be used as an additional prognostic tool for early pregnancy diagnosis, and we particularly focused on the differences in LBF between pregnant and nonpregnant mares. Furthermore, other possible developmental differences of corpus luteum (CL) between pregnant and nonpregnant mares were evaluated. The CL (n = 119) of 27 mares were monitored once daily in B- and Power-Doppler Mode on days 1, 2, 9, 12, and 16 after ovulation (day 0 = ovulation). The data were evaluated using the MIXED Linear Model with repeated measures, and parameters were estimated by the REML method. The course of LBF, area of CL, and pixel intensity differed in nonpregnant mares on a day-to-day basis in contrast to more stable values in pregnant mares. Further, the profiles of the courses were identical until day 9, but since day 12 the differences between pregnant and nonpregnant mares started to be prominent. The LBF, pixel intensity, and level of progesterone (P4) were similar in all mares until day 16, when smaller LBF, lower pixel intensity, and lower levels of P4 were found in nonpregnant mares (P = 0.04, P = 0.02, P < 0.05, respectively). In pregnant and nonpregnant mares the LBF was weakly (r = 0.29 in both) and pixel intensity strongly (r = 0.48 and 0.59, respectively) correlated to the levels of P4. LBF was strongly correlated to the area of CL in pregnant as well as nonpregnant mares (r = 0.72 and 0.64, respectively). In accordance with the results presented in our study we can state that LBF monitoring is not a suitable tool for early pregnancy diagnosis or prognosis as the differences between pregnant and nonpregnant mares are notable – similarly to other indicators of CL status – just after the onset of luteolysis (day 16) when embryo itself is detectable.  

Corpus luteum blood flow in abnormal early pregnancy.

1996 ◽  
Vol 15 (9) ◽  
pp. 645-649 ◽  
Author(s):  
J L Alcázar ◽  
C Laparte ◽  
G López-Garcia
Keyword(s):  
Blood Flow ◽  
Corpus Luteum ◽  
Early Pregnancy

Post-Radical Prostatectomy Penile Blood Flow: Assessment with Color Flow Doppler Ultrasound

1994 ◽  
Vol 152 (6 Part 2) ◽  
pp. 2276-2279 ◽  
Author(s):  
Edward D. Kim ◽  
Donna Blackburn ◽  
Kevin T. McVary
Keyword(s):  
Blood Flow ◽  
Radical Prostatectomy ◽  
Doppler Ultrasound ◽  
Color Flow

314 EARLY PREGNANCY DIAGNOSIS USING A NOVEL TRANSRECTAL ULTRASONOGRAPHY PROTOCOL IN JAPANESE BLACK COWS

2009 ◽  
Vol 21 (1) ◽  
pp. 254
Author(s):  
A. Gaja ◽  
C. Kubota ◽  
T. Kojima
Keyword(s):  
Corpus Luteum ◽  
Early Pregnancy ◽  
Transrectal Ultrasonography ◽  
Blood Collection ◽  
Sectional Area ◽  
Cross Sectional ◽  
Plasma Progesterone ◽  
Area Change ◽  
Pregnancy Diagnosis ◽  
Peripheral Plasma

The present study aims to establish a novel practical protocol for early pregnancy diagnosis in cows by using transrectal ultrasonography. The protocol is based on measurements of corpus luteum (CL) cross-sectional area (CL c-s area) change performed at 2 separate days before the coming estrus after AI. Fourteen cows were inseminated artificially, and transrectal ultrasonographical observation of the ovaries and blood collection for measurement of peripheral plasma progesterone (P4) concentration were carried out daily from Days 12 to 23 (Day 0 = the day of onset of estrus). Thereafter, cows were routinely diagnosed for pregnancy at Day 30 by transrectal ultrasonography. The largest CL c-s area was obtained at Day 14 in both pregnant and non-pregnant cows. Seven out of 8 non-pregnant cows showed significant CL c-s area regression between Days 14 and 20 (422 ± 112 v. 249 ± 63 mm2), whereas no regression was observed between Days 14 and 20 in pregnant cows (416 ± 65 v. 402 ± 78 mm2). The regression in the CL c-s area between pregnant and non-pregnant cows was significantly different during Day 18 (424 ± 65 v. 288 ± 88 mm2) to Day 23 (402 ± 71 v. 139 ± 64 mm2). P4 concentration was significantly low (less than 1 ng mL–1) at Day 20 in 3 out of 8 non-pregnant cows, whereas the pregnant cows showed significant increase of P4 between Days 14 and 20 (2.6 ± 0.2 v. 3.4 ± 0.5 ng mL–1). The pregnant cows showed significantly higher P4 concentration starting from Day 18 than non-pregnant cows. However, in non-pregnant cows, 4 cows returned to estrus on Day 20 or after, 3 cows showed no signs of estrus, and 1 cow came in estrus as early as Day 18 after AI. In conclusion, the results of the present study suggest that measuring the change in the CL c-s area at Days 14 and 20 makes it possible to detect the non-pregnant cows at Day 20 after AI. However, it was also indicated that measuring the change of P4 concentrations on the same days did not always successfully detect non-pregnant cows. The new protocol based on CL c-s area regression rate can detect almost certainly non-pregnant cows at Day 20 after AI. It is suggested that this method is advantageous in research and industrial breeding.

16 False-positive rate after anticipation of early pregnancy diagnosis for resynchronization of ovulation in Bos indicus heifers

2019 ◽  
Vol 31 (1) ◽  
pp. 134
Author(s):  
L. M. S. Simões ◽  
E. A. Lima ◽  
A. P. C. Santos ◽  
R. E. Orlandi ◽  
M. P. Bottino ◽  
...  
Keyword(s):  
Blood Flow ◽  
Pregnancy Rate ◽  
Doppler Ultrasound ◽  
False Positive ◽  
Doppler Ultrasonography ◽  
Bos Indicus ◽  
False Positives ◽  
Colour Doppler ◽  
Pregnancy Diagnosis ◽  
Colour Doppler Ultrasonography

The objective was to determine the incidence of false-positive pregnancy diagnosis following the use of colour Doppler ultrasonography 20 and 22 days after fixed-time AI (FTAI) in Bos indicus heifers submitted to resynchronization 14 days after first FTAI. In the study, 512 Nellore heifers at 24.5±0.8 months of age and body condition score of 3.4±0.1 were used beginning 14 days after FTAI. On Day 14, heifers received 50mg of short-acting progesterone (Afisterone®, CEVA, São Paulo, Brazil) and a progesterone device (Prociclar®, CEVA). Eight days later (Day 22), the progesterone device was removed. A duplex B-mode (grayscale) and pulse-wave colour Doppler ultrasound instrument (M5, Mindray, Shenzhen, China) equipped with a multifrequency linear transducer was used for the examination of luteal blood flow on Days 20 and 22. The percentage of luteal area with colour Doppler signals of blood flow at each examination was determined as previously described (Ginther 2007) and was classified as low [corpus luteum (CL) with less than 25% vascularized area], intermediate (CL with 25-75% vascularized area), or high (CL with more than 75% vascularized area). Heifers with intermediate and high luteal blood flow were diagnosed as pregnant by Doppler ultrasonography, and heifers with low luteal blood flow were diagnosed as nonpregnant. Heifers diagnosed as pregnant by Doppler ultrasonography were examined 30 days after FTAI by B-mode ultrasound examination for pregnancy diagnosis and to determine the number of false positives (heifers pregnant by Doppler ultrasonography and nonpregnant by B-mode ultrasonography). Statistical analysis was performed by the GLIMMIX procedure of SAS (SAS Institute Inc., Cary, NC, USA). Pregnancy rate by Doppler ultrasonography [Day 20=60.5% (310/512), Day 22=55.3% (283/512); P=0.10] and false-positive percentage [Day 20=29.7% (92/310), Day 22=23.0% (65/2830); P=0.06] were similar on pregnancy diagnoses by Doppler ultrasonography performed 20 and 22 days after FTAI. Furthermore, in the period from 20 to 22 days after FTAI, luteolysis was verified in 5.8% (30/512) of heifers. In conclusion, anticipation of pregnancy diagnosis in 2 days by evaluation of luteal blood flow with colour Doppler ultrasonography (20 days after FTAI) does not interfere with pregnancy rate by Doppler ultrasound and percentage of false positives in Bos indicus heifers.

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