Artificial insemination at 56 h after intravaginal progesterone device removal improved AI pregnancy rate in beef heifers synchronized with five-day CO-Synch + controlled internal drug release (CIDR) protocol

2012 ◽  
Vol 77 (8) ◽  
pp. 1624-1631 ◽  
Author(s):  
R. Kasimanickam ◽  
M. Asay ◽  
P. Firth ◽  
W.D. Whittier ◽  
J.B. Hall
Keyword(s):  
Drug Release ◽  
Pregnancy Rate ◽  
Artificial Insemination ◽  
Device Removal ◽  
Beef Heifers

Efficiency of mating, artificial insemination or resynchronisation at different times after first timed artificial insemination in postpartum Nellore cows to produce crossbred calves

2019 ◽  
Vol 59 (2) ◽  
pp. 225 ◽  
Author(s):  
Walvonvitis Baes Rodrigues ◽  
Jean do Prado Jara ◽  
Juliana Correa Borges ◽  
Luiz Orcirio Fialho de Oliveira ◽  
Urbano Pinto Gomes de Abreu ◽  
...  
Keyword(s):  
Pregnancy Rate ◽  
Breeding Season ◽  
Chorionic Gonadotropin ◽  
Artificial Insemination ◽  
Fixed Time ◽  
Beef Cows ◽  
Device Removal ◽  
Oestradiol Benzoate ◽  
Timed Artificial Insemination ◽  
Equine Chorionic Gonadotropin

The objective of this trial was to evaluate different post-timed artificial insemination (TAI) reproductive managements in postpartum beef cows to produce crossbred calves from artificial insemination (AI). Nellore cows (n = 607), with 45 days postpartum, were inseminated at a fixed time, using a protocol that included an intravaginal progesterone-releasing device along with oestradiol benzoate, prostaglandin, equine chorionic gonadotropin, and oestradiol cypionate, followed TAI 48 h post-device removal. Four post-TAI treatments were evaluated: in CONTROL (T1, n = 161), cows were exposed to Nellore clean-up bulls until the end of the breeding season (75 days). In OBSERVATION (T2, n = 132), heat detection was performed for 15–25 days post-TAI, followed by AI. In RESYNC22 (T3, n = 157) and RESYNC30 (T4, n = 157), resynchronisation started after 22 or 30 days, following second TAI at Day 32 or 40 days after first TAI. In T2, T3 and T4, after the second AI, cows were exposed to Nellore clean-up bulls until the end of the breeding season (75 days). The pregnancy rate (PR) for the first TAI did not differ (54.6%, 53.0%, 59.2%, and 51.6% for CONTROL, OBSERVATION, RESYNC 22, and RESYNC 30, respectively; P = 0.66), and no difference was observed for the second TAI (RESYNC 22 = 45.31% and RESYNC30 = 46.05%; P = 0.137), in the PR at the end of the breeding season (86.33%, 86.36%, 78.98%, and 81.52%, P = 0.43), or embryonic losses (4.54%, 2.85%, 6.45% and 7.40%, respectively; P = 0.61), but the percentage of crossbred pregnancy was higher in groups with resynchronisation (RESYNC22 and RESYNC30) than CONTROL and OBSERVATION (98.38%, 90.62%, 63.30%, 78.95%, P < 0.001). In conclusion, resynchronisation programs of 22 or 30 days are more efficient to produce AI products, and the final pregnancy rate is similar among the treatments, differing only in the amount of calves produced by AI.

118 A comparison of intravaginal progesterone devices for fixed-time artificial insemination in beef cattle

2021 ◽  
Vol 33 (2) ◽  
pp. 167
Author(s):  
E. M. Zwiefelhofer ◽  
S. X. Yang ◽  
M. Asai-Coakwell ◽  
M. G. Colazo ◽  
J. Hellquist ◽  
...  
Keyword(s):  
Artificial Insemination ◽  
Animal Health ◽  
Colour Change ◽  
Fixed Time ◽  
Device Removal ◽  
Beef Heifers ◽  
Lactating Cows ◽  
Pregnancy Status ◽  
Gonadotrophin Releasing Hormone ◽  
Follicular Dynamics

Intravaginal progesterone (P4) devices used for ovarian synchronization before fixed-time AI (FTAI) differ in drug release, which may influence fertility outcome. A 2×2 study was designed to determine the effects of different intravaginal devices (PRID Delta, 1.55g of P4 vs. CIDR, 1.38g of P4) and parity (heifers vs. cows) on follicular dynamics, expression of oestrus, and pregnancy per AI (P/AI). At random stages of the oestrous cycle, nulliparous beef heifers and lactating cows were given 100µg of gonadorelin (gonadotrophin-releasing hormone, GnRH) intramuscularly (IM) and assigned randomly to either the PRID (n=76 heifers, 76 multiparous, 27 primiparous) or CIDR (n=76 heifers, 73 multiparous, 32 primiparous) group. Devices were removed 5 days later, an oestrus-detection patch was applied just cranial to the tail head, and 500µg of cloprostenol was given IM at the time of device removal and again 24h later. At 72h after device removal, cattle were inseminated and given 100µg of GnRH IM. Transrectal ultrasonography was used to determine the diameter of the largest follicle on the day of device removal and at FTAI, ovulation time, and pregnancy status 30 days after FTAI. A colour change of ≥50% of the oestrus-detection patch at FTAI was taken as expression of oestrus. Data were compared among groups by 2-way ANOVA using MIXED and GLIMMIX procedures. There were no interactions between P4 device and parity for any endpoint. The diameter of the largest follicle (mean±s.e.m.) was not different between PRID and CIDR groups on either the day of device removal (10.6±0.1 vs. 10.9±0.1mm) or the day of FTAI (13.7±0.1 vs. 13.9±0.1mm). The proportion displaying oestrus did not differ between P4 device groups, but was greater in heifers than in cows [121/152 (79.6%) vs. 135/207 (65.2%); P<0.01], and the interval from FTAI to ovulation was shorter in heifers than in cows (27.8±1.2 vs. 32.0±1.1 h; P=0.01). The P/AI was not different between P4 device groups or parity groups (overall 67.0%, 238/355). However, among lactating cows, the P/AI tended to be greater in the PRID vs. CIDR group [75/102 (73.5%) vs. 64/105 (61.0%); P=0.10], and was greater in multiparous vs. primiparous cows [106/148 (71.6%) vs. 33/59 (55.9%); P=0.04]. Among cattle that displayed oestrus, the P/AI tended to be greater in the PRID vs. CIDR group [92/123 (74.8%) vs. 85/131 (64.9%); P=0.09]. Among lactating cows that displayed oestrus, the P/AI was greater in multiparous vs. primiparous cows [74/94 (78.2%) vs. 24/42 (57.1%); P<0.01]. In summary, follicular dynamics and expression of oestrus did not differ between PRID and CIDR groups, but the P/AI tended to be greater in PRID-treated lactating cows and in cattle that displayed oestrus. This research was supported by CEVA Animal Health, Saskatchewan ADF, Agriculture and Agri-Foods Canada, and Rockway Inc.

scholarly journals PSII-21 Vaginitis incidence and effects on fertility of beef females enrolled on estrus synchronization protocols using a controlled internal drug release (CIDR) device

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 242-242 ◽  
Author(s):  
Nicholas W Dias ◽  
Alvaro Sales ◽  
Claire Timlin ◽  
Stefania Pancini ◽  
John Currin ◽  
...  
Keyword(s):  
Drug Release ◽  
Pregnancy Rate ◽  
Artificial Insemination ◽  
Pregnancy Rates ◽  
Estrus Synchronization ◽  
Pregnancy Status ◽  
Timed Artificial Insemination ◽  
System 1 ◽  
Synchronization Protocol

Abstract Our objectives were to characterize the incidence of vaginitis caused by a controlled internal drug release (CIDR) device and its effects on fertility of beef females enrolled on estrus synchronization followed by timed-artificial insemination (TAI). A total of 1,097 mature cows and 215 heifers from 9 different locations were enrolled in the 7-d CO-Synch + CIDR synchronization protocol. At CIDR insertion, all females were inspected for any previous signs of vaginitis prior to enrollment in the experiment. As evidence of vaginitis, a CIDR score was determined at CIDR removal, and an AI plastic sheath score was also performed at TAI following a 1 to 4 system: 1=clean presenting no secretion; 2=clean presenting clear secretion; 3=presence of purulent secretion; 4=presence of purulent secretion and blood. Pregnancy status was determined by rectal ultrasonography approximately 40 days after TAI. The scores for CIDR and AI plastic sheath did not differ between cows and heifers. Of all females, 6% had a CIDR score of 1, 31% score 2, 56% score 3, and 7% score 4. Plastic sheath score was 1 for 16%, 2 for 62%, 3 for 15%, and 4 for 7% of females. Pregnancy rate to TAI differed between cows and heifers (P < 0.01), among locations (P < 0.001) and ranged from 36 to 66%; however, it was not influenced by CIDR score (P = 0.259) and plastic sheath score (P = 0.785). Pregnancy rate of females by different CIDR scores was 60%, 57%, 52%, and 46% for scores 1, 2, 3, and 4, respectively. Pregnancy rate of females by different AI plastic sheath score was 60%, 55%, 45%, and 48%, for scores 1, 2, 3, and 4, respectively. The presence of vaginitis caused by a CIDR does not influence pregnancy rates of TAI in beef females.

scholarly journals Effect of two prostaglandin F2α injections administered 24 hours apart on the pregnancy rate of Simmental cows subjected to the Ovsynch or Ovsynch + Controlled internal drug release (CIDR) protocols

2020 ◽  
Vol 76 (10) ◽  
pp. 6451-2020
Author(s):  
MUSHAP KURU ◽  
CIHAN KACAR ◽  
HASAN ORAL ◽  
SEMRA KAYA ◽  
NEBI CETIN ◽  
...  
Keyword(s):  
Drug Release ◽  
Pregnancy Rate ◽  
Artificial Insemination ◽  
Pregnancy Loss ◽  
Prostaglandin F2α ◽  
Fixed Time ◽  
Group 4 ◽  
Group 3 ◽  
Group 2 ◽  
Group 1

The aim of the present study was to determine the effects of one or two doses of prostaglandin F2α (PGF) on the pregnancy rate (PR) in Simmental cows subjected to the Ovsynch or Ovsynch + Controlled internal drug release (CIDR) protocols. On d 0, 100 µg gonadorelin diacetate tetrahydrate (GnRH) was injected into cows in group 1 (n = 50) and group 2 (n = 40) and CIDR was intravaginally inserted. On d 7, 25 mg dinoprost tromethamine (PGF2α, PGF) was administered, and GnRH was injected 56 h later. Fixed-time artificial insemination (TAI) was performed 16 h after GnRH injection. In group 3 (n = 62) and group 4 (n = 63), PGF was administered 7 d following GnRH treatment, and GnRH was injected 56 h later. TAI was performed 16 h after GnRH injection. In groups 2 and 4, a second dose of PGF was injected 24 h after the first dose. Pregnancy was determined by transrectal ultrasonography on d 30 and 60 post-TAI. The pregnancy rate (PR) on d 30 post-TAI was significantly different among the groups: 46%, 55%, 29%, and 36.5%, in groups 1, 2, 3, and 4, respectively (P = 0.048). In addition, the PR was 36.3% and 43.7% in the single PGF and double PGF injection groups, respectively. There was no statistical difference in the PR on d 60 post-TAI (P > 0.05). Pregnancy loss was between 4.34% and 11.11% (P > 0.05). The highest PR in the primiparous cows was observed in group 3 (57.7%). The PR was not significantly different among multiparous cows (P > 0.05). In conclusion, two injections of PGF administered 24 h apart in Simmental cows may increase the PR in the Ovsynch or Ovsynch + CIDR protocols.

22 PREGNANCY RATES IN DAIRY COWS TREATED WITH PROGESTERONE-RELEASING DEVICES AND DIFFERENT GONADOTROPIN-RELEASING HORMONE OR ESTRADIOL PLUS EQUINE CHORIONIC GONADOTROPIN FIXED-TIME ARTIFICIAL INSEMINATION PROTOCOLS

2008 ◽  
Vol 20 (1) ◽  
pp. 91 ◽  
Author(s):  
G. Veneranda ◽  
L. Filippi ◽  
D. Racca ◽  
L. Cutaia ◽  
G. A. Bo
Keyword(s):  
Dairy Cows ◽  
Pregnancy Rate ◽  
Milk Yield ◽  
Artificial Insemination ◽  
Fixed Time ◽  
Gonadotropin Releasing Hormone ◽  
Pregnancy Rates ◽  
Device Removal ◽  
Treatment Groups ◽  
Lactating Dairy Cows

We have previously shown that the addition of eCG improved pregnancy rates following fixed-time artificial insemination (FTAI) in lactating dairy cows treated with estradiol benzoate (EB) and progesterone releasing devices (Veneranda et al. 2006 Reprod. Fertil. Dev. 18, 118). An experiment was designed to compare pregnancy rates in lactating dairy cows treated with progesterone-releasing devices and EB plus eCG with different gonadotropin-releasing hormone (GnRH)-based protocols. Five-hundred lactating dairy cows between 30 and 51 days postpartum (when prostaglandin F (PGF) treatments were given in the Pre-Synch groups), with a milk yield of 29.5 � 7.0 kg per day (range 13.0 to 46.0 kg) and a body condition score (BCS) between 2.5 to 3.5 out of 5 were used. Cows were blocked by days postpartum and randomly assigned to five treatment groups. Cows in the Pre-Synch group received PGF 28 and 14 days prior to the insertion of a DIB device (1 g progesterone, Syntex SA, Buenos Aires, Argentina) and the administration of 50 µg of Lecirelin (GnRH, Ovusin, Syntex SA) IM (Day 0). On Day 7, DIB were removed and cows received PGF (0.5 mg cloprostenol, Ciclase, Syntex SA). On Day 9, cows received a second GnRH treatment and were FTAI 16 h later (60 h after device removal). Cows in the Modified Pre-Synch group were treated similarly except that they received a DIB for 7 days prior to the second PGF injection and no DIB at the time of the first GnRH. Cows in the P4-Synch group were treated as those in the Pre-synch group but did not receive the two doses of PGF 28 and 14 days earlier. Cows in the P4 + EB + eCG groups received a DIB for 8 days, 2 mg of EB at DIB insertion (Day 0), and PGF and 400 IU eCG (Novormon, Syntex SA) IM at DIB removal (Day 8); they were subdivided to receive 1 mg of EB on Day 9 (P4 + EB + eCG + EB group) or GnRH on Day 10 (P4 + EB + eCG + GnRH group). Cows in these latter two groups were also FTAI 60 h after device removal. Pregnancy was determined by rectal palpation 50 days after FTAI and data were analyzed by logistic regression. Pregnancy rates were not affected by BCS, days postpartum, or milk yield (P > 0.5). However, there was a significant group effect due to a higher (P < 0.05) pregnancy rate in the P4 + EB + eCG + EB (49%) and P4 + EB + eCG + GnRH (44%) treatment groups than in those in the Modified Pre-Synch group (30%). Pregnancy rate in the P4-Synch group (39%) was lower (P < 0.05) than those in the P4 + EB + eCG + EB group but not different from the others. Finally, pregnancy rate in Pre-Synch group (41%) was intermediate and not different from the other treatment groups. Results of these experiments suggest that treatments with progesterone-releasing devices, EB, and eCG result in pregnancy rates that are comparable to or better than those obtained in GnRH-based protocols in lactating dairy cows. Further studies are needed to investigate whether eCG could increase pregnancy rates in cows treated with GnRH and progesterone-releasing devices. The authors thank Syntex SA for providing the hormones used in the study.

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