Comparative changes in gene expression profiles induced by phenobarbital, troglitazone and aflatoxin B1 in primary human hepatocytes and HepaRG cells

2009 ◽  
Vol 189 ◽  
pp. S71
Author(s):  
Alexandra Rogue ◽  
Rozenn Jossé ◽  
Carine Lambert ◽  
Catherine Spire ◽  
Nancy Claude ◽  
...  
Keyword(s):  
Gene Expression ◽  
Aflatoxin B1 ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Human Hepatocytes ◽  
Primary Human Hepatocytes ◽  
Heparg Cells

Baseline and genotoxic compound induced gene expression profiles in HepG2 and HepaRG compared to primary human hepatocytes

2013 ◽  
Vol 27 (7) ◽  
pp. 2031-2040 ◽  
Author(s):  
M.J.A. Jetten ◽  
J.C.S. Kleinjans ◽  
S.M. Claessen ◽  
C. Chesné ◽  
J.H.M. van Delft
Keyword(s):  
Gene Expression ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Human Hepatocytes ◽  
Primary Human Hepatocytes

Interindividual Variability in Gene Expression Profiles in Human Hepatocytes and Comparison with HepaRG Cells

2011 ◽  
Vol 40 (1) ◽  
pp. 151-158 ◽  
Author(s):  
Alexandra Rogue ◽  
Carine Lambert ◽  
Catherine Spire ◽  
Nancy Claude ◽  
André Guillouzo
Keyword(s):  
Gene Expression ◽  
Interindividual Variability ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Human Hepatocytes ◽  
Heparg Cells

Long-term functional maintenance of primary human hepatocytes in vitro

Science ◽  
2019 ◽  
Vol 364 (6438) ◽  
pp. 399-402 ◽  
Author(s):  
Chengang Xiang ◽  
Yuanyuan Du ◽  
Gaofan Meng ◽  
Liew Soon Yi ◽  
Shicheng Sun ◽  
...  
Keyword(s):  
Cell Biology ◽  
Expression Profiles ◽  
Antiviral Drug ◽  
Gene Expression Profiles ◽  
Global Gene Expression ◽  
Human Hepatocytes ◽  
Hbv Infection ◽  
Primary Human Hepatocytes

The maintenance of terminally differentiated cells, especially hepatocytes, in vitro has proven challenging. Here we demonstrated the long-term in vitro maintenance of primary human hepatocytes (PHHs) by modulating cell signaling pathways with a combination of five chemicals (5C). 5C-cultured PHHs showed global gene expression profiles and hepatocyte-specific functions resembling those of freshly isolated counterparts. Furthermore, these cells efficiently recapitulated the entire course of hepatitis B virus (HBV) infection over 4 weeks with the production of infectious viral particles and formation of HBV covalently closed circular DNA. Our study demonstrates that, with a chemical approach, functional maintenance of PHHs supports long-term HBV infection in vitro, providing an efficient platform for investigating HBV cell biology and antiviral drug screening.

scholarly journals Comparative Gene Expression Profiles Induced by PPARγ and PPARα/γ Agonists in Human Hepatocytes

PLoS ONE ◽  
2011 ◽  
Vol 6 (4) ◽  
pp. e18816 ◽  
Author(s):  
Alexandra Rogue ◽  
Carine Lambert ◽  
Rozenn Jossé ◽  
Sebastien Antherieu ◽  
Catherine Spire ◽  
...  
Keyword(s):  
Gene Expression ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Human Hepatocytes

scholarly journals 3D spheroid cultures improve the metabolic gene expression profiles of HepaRG cells

2015 ◽  
Vol 35 (3) ◽  
Author(s):  
Yu Takahashi ◽  
Yuji Hori ◽  
Tomohisa Yamamoto ◽  
Toshiki Urashima ◽  
Yasunori Ohara ◽  
...  
Keyword(s):  
Gene Expression ◽  
Apolipoprotein B ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Hanging Drop ◽  
Albumin Secretion ◽  
3D Cultures ◽  
Metabolic Gene ◽  
Metabolic Gene Expression ◽  
Heparg Cells

Apo (Apolipoprotein)B secretion, as well as albumin secretion, increased in 3D HepG2 and HepaRG spheroids. Liver metabolic gene expression was up-regulated in 3D HepaRG spheroids. These results suggest that hanging drop 3D cultures can improve hepatocellular responses as a functional liver.

Reproducible chemical-induced changes in gene expression profiles in human hepatoma HepaRG cells under various experimental conditions

2009 ◽  
Vol 23 (3) ◽  
pp. 466-475 ◽  
Author(s):  
Carine B. Lambert ◽  
Catherine Spire ◽  
Marie-Pierre Renaud ◽  
Nancy Claude ◽  
Andre Guillouzo
Keyword(s):  
Gene Expression ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Human Hepatoma ◽  
Experimental Conditions ◽  
Heparg Cells ◽  
Induced Changes
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