A Comparison of Whole Genome Gene Expression Profiles of HepaRG Cells and HepG2 Cells to Primary Human Hepatocytes and Human Liver Tissues

The maintenance of terminally differentiated cells, especially hepatocytes, in vitro has proven challenging. Here we demonstrated the long-term in vitro maintenance of primary human hepatocytes (PHHs) by modulating cell signaling pathways with a combination of five chemicals (5C). 5C-cultured PHHs showed global gene expression profiles and hepatocyte-specific functions resembling those of freshly isolated counterparts. Furthermore, these cells efficiently recapitulated the entire course of hepatitis B virus (HBV) infection over 4 weeks with the production of infectious viral particles and formation of HBV covalently closed circular DNA. Our study demonstrates that, with a chemical approach, functional maintenance of PHHs supports long-term HBV infection in vitro, providing an efficient platform for investigating HBV cell biology and antiviral drug screening.

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Gene Expression Profiles of Early Implant Adherent Cells in Smokers and Nonsmokers

Journal of Oral Implantology ◽

10.1563/aaid-joi-d-13-00266 ◽

2015 ◽

Vol 41 (6) ◽

pp. 640-645 ◽

Cited By ~ 6

Author(s):

Ghadeer Thalji ◽

Lyndon F. Cooper ◽

Salvador Nares

Keyword(s):

Gene Expression ◽

Early Stage ◽

Expression Profiles ◽

Gene Expression Profiles ◽

Whole Genome ◽

Adherent Cells ◽

Mini Implants ◽

Molecular Events ◽

The Impact

The objective of this study was to evaluate the impact of smoking on the early molecular events involved in peri-implant healing at either a micro-roughened or a micro-roughened with superimposed nanofeatures surface implant in humans. Twenty-one subjects, 10 smokers and 11 nonsmokers received 4 mini-implants (2.2 × 5.0 mm; 2 of each surface). After 3 and 7 days, paired mini-implants were retrieved by reverse threading and RNA isolated from implant adherent cells. Whole genome microarrays were used interrogate the gene expression profiles. The study failed to identify differences in the gene expression profiles of implant adherent cells at this early stage of osseointegration (up to day 7) comparing smoker and nonsmoker individuals.

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Gene expression profiles in human HepG2 cells treated with extracts of the Tamarindus indica fruit pulp

Genes & Nutrition ◽

10.1007/s12263-010-0187-5 ◽

2010 ◽

Vol 5 (4) ◽

pp. 331-341 ◽

Cited By ~ 11

Author(s):

Nurhanani Razali ◽

Azlina A. Aziz ◽

Sarni M. Junit

Keyword(s):

Gene Expression ◽

Hepg2 Cells ◽

Expression Profiles ◽

Gene Expression Profiles ◽

Fruit Pulp ◽

Tamarindus Indica

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Relationship Between San-Huang-Xie-Xin-Tang and Its Herbal Components on the Gene Expression Profiles in HepG2 Cells

The American Journal of Chinese Medicine ◽

10.1142/s0192415x08006235 ◽

2008 ◽

Vol 36 (04) ◽

pp. 783-797 ◽

Cited By ~ 43

Author(s):

Wen-Yu Cheng ◽

Shih-Lu Wu ◽

Chien-Yun Hsiang ◽

Chia-Cheng Li ◽

Tung-Yuan Lai ◽

...

Keyword(s):

Gene Expression ◽

Hepg2 Cells ◽

Expression Profiles ◽

Gene Expression Profiles ◽

Enrichment Analysis ◽

Major Effect ◽

Gene Set Enrichment Analysis ◽

P53 Signaling ◽

The Relationship ◽

Herbal Components

Traditional Chinese medicine (TCM) has been used for thousands of years. Most Chinese herbal formulae consist of several herbal components and have been used to treat various diseases. However, the mechanisms of most formulae and the relationship between formulae and their components remain to be elucidated. Here we analyzed the putative mechanism of San-Huang-Xie-Xin-Tang (SHXXT) and defined the relationship between SHXXT and its herbal components by microarray technique. HepG2 cells were treated with SHXXT or its components and the gene expression profiles were analyzed by DNA microarray. Gene set enrichment analysis indicated that SHXXT and its components displayed a unique anti-proliferation pattern via p53 signaling, p53 activated, and DNA damage signaling pathways in HepG2 cells. Network analysis showed that most genes were regulated by one molecule, p53. In addition, hierarchical clustering analysis showed that Rhizoma Coptis shared a similar gene expression profile with SHXXT. These findings may explain why Rhizoma Coptis is the principle herb that exerts the major effect in the herbal formula, SHXXT. Moreover, this is the first report to reveal the relationship between formulae and their herbal components in TCM by microarray and bioinformatics tools.

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