Evaluation of cytotoxicity of chitosan/alginate nanoparticles to encapsulate antisense oligonucleotides for inhibition of EGFR expression in breast cancer T47D cell line

Background: Breast cancer is the second leading cause of death in women. Alternative medicine with high efficacy is needed for breast cancer treatments, for example induction of apoptosis using natural products. It has been found that many natural apoptosis-inducing compounds are isolated from marine sponge. The objective of this study is to analyze the ability of extracts of the sponge Ancorina sp. to induce apoptosis on human breast cancer T47D cell line and find out its mechanism. Methods: T47D cells were treated with crude extracts of methanol, dichloromethane:methanol (1:1) and dichloromethane Ancorina sp. for 24 h, and doxorubicin was used as a positive control. Methods used for this study were MTT assay to examine cell viability and determine IC50 of the three extracts, while the percentage of apoptosis and caspase-3 were investigated by flow cytometry. Results: IC50 values of methanol, dichloromethane:methanol (1:1), and dichloromethane extract were 84.25, 121.45, and 99.85μg/mL respectively. The percentages of apoptotic cells after treatment with methanol, dichloromethane:methanol (1:1), and dichloromethane extracts were 88.68, 27.54 and 53.63% respectively, whereas the percentage of caspase-3 was 77.87, 12.66 and 12.97%, respectively. Conclusions: These results revealed that all extracts of Ancorina sp. have strong or moderate cytotoxicity and have the ability to induce apoptosis on T47D human breast cancer cell line. However, methanol crude extract has high efficacy to induce apoptosis through caspase-3 activation compared to the other extracts. Hence methanol extract warrants further investigation as a natural medicine for human breast cancer.

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THE ACTIVE FRACTION FROM Nigella sativa AND ITS ACTIVITY AGAINST T47D CELL LINE

Indonesian Journal of Chemistry ◽

10.22146/ijc.21383 ◽

2011 ◽

Vol 11 (3) ◽

pp. 217-222 ◽

Cited By ~ 2

Author(s):

Heny Ekowati ◽

Eka Prasasti ◽

Undri Rastuti

Keyword(s):

Breast Cancer ◽

Side Effects ◽

Cytotoxic Activity ◽

Cell Line ◽

Nigella Sativa ◽

T47d Cell ◽

Chloroform Extract ◽

The Third ◽

T47d Cell Line ◽

Better Than

Breast cancer is one of the main causes of death in women. Cancer treatment with surgery, chemotherapy, and radiology often cause undesirable side effects. Therefore, alternative cancer treatment by using plants as traditional medicine was expected to reduce side effects. Nigella sativa is one of the plants used as anticancer empirically. This study conducted to examine the cytotoxic activity of Nigella sativa seeds and identify its components on T47D breast cancer cells. Petroleum ether, chloroform, ethyl acetate, and ethanol were used to extract N. sativa seeds. The extracts were tested their cytotoxic activity on T47D cell line using MTT method. The active compound was separated using column chromatography. Cytotoxic test on T47D cell line was perform for extracts of each separation stage. Data were analyzed by probit analysis to obtain IC50 values. Components identification was performed using GC-MS analysis. The results showed that chloroform extract has cytotoxic activity better than other extracts with IC50 of 124.206 µg/mL. The third fraction has cytotoxic activity better than other fractions with IC50 of 68.568 µg/mL. The GC-MS analysis showed that in the third fraction of the chloroform extract contain linoleat acid, the major compound and tryptamine.

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The T47D cell line is an ideal experimental model to elucidate the progesterone-specific effects of a luminal A subtype of breast cancer

Biochemical and Biophysical Research Communications ◽

10.1016/j.bbrc.2017.03.114 ◽

2017 ◽

Vol 486 (3) ◽

pp. 752-758 ◽

Cited By ~ 18

Author(s):

Sungryul Yu ◽

Taemook Kim ◽

Kyung Hyun Yoo ◽

Keunsoo Kang

Keyword(s):

Breast Cancer ◽

Cell Line ◽

Experimental Model ◽

T47d Cell ◽

Luminal A ◽

Specific Effects ◽

T47d Cell Line

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Anticancer effect of ethanol Lycium barbarum (Goji berry) extract on human breast cancer T47D cell line

Natural Product Research ◽

10.1080/14786419.2015.1101691 ◽

2015 ◽

Vol 30 (17) ◽

pp. 1993-1996 ◽

Cited By ~ 18

Author(s):

Anna Wawruszak ◽

Arkadiusz Czerwonka ◽

Karolina Okła ◽

Wojciech Rzeski

Keyword(s):

Breast Cancer ◽

Cell Line ◽

Human Breast Cancer ◽

T47d Cell ◽

Human Breast ◽

Anticancer Effect ◽

Lycium Barbarum ◽

Goji Berry ◽

T47d Cell Line

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Cytotoxicity Assay From Fractions of Hedyotis corymbosa Extract Against Breast Cancer Cell Line T47D

Indonesian Journal of Cancer Chemoprevention ◽

10.14499/indonesianjcanchemoprev2iss1pp182-186 ◽

2011 ◽

Vol 2 (1) ◽

pp. 182

Author(s):

Rina Andriyani ◽

Chandra Risdian ◽

Zalinar Udin

Keyword(s):

Breast Cancer ◽

Herbal Medicine ◽

Cell Line ◽

Methylene Chloride ◽

Cancer Cell Line ◽

Ethanolic Extract ◽

T47d Cell ◽

Methylene Chloride Extract ◽

T47d Cell Line ◽

Cancer Medicine

Drug discovery for cancer medication is the most important effort that researcher do at this time. Indonesia bio diversities have possibility as a cancer medicine sources. Finding a herbal medicine for cancer treatment is a first step to find a right cancer medicine in the future. This research has already completed for the earlier another research. Some fractions of Hedyotis corymbosa extract has been analyzed using Sulforhodamine B method with UV wavelength 515 nm against T47D cell line, a human breast cancer. There are Hexane extract, Methylene chloride extract and Ethyl acetate extract, and give inhibitory concentration 50 (IC50) of 33.45 µg/ml, 54.59 µg/ml and 52.58 µg/ml, respectively. Ethanolic extract, itself has IC50 of 61.57 µg/ml, whereas IC50 value of Cisplatinum is 9.63 µg/ml. There is a difference between the ethanolic extracts with the other fraction.Keywords: breast cancer, herbal medicine, T47D, Hedyotis corymbosa

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Sitotoksisitas in vitro ekstrak etanolik buah parijoto (Medinilla speciosa, reinw.ex bl.) terhadap sel kanker payudara T47D

JURNAL GIZI INDONESIA ◽

10.14710/jgi.2.2.53-58 ◽

2014 ◽

Vol 2 (2) ◽

pp. 53-58 ◽

Cited By ~ 1

Author(s):

Iin Tusanti ◽

Andrew Johan ◽

RA Kisdjamiatun

Keyword(s):

Breast Cancer ◽

Cytotoxic Activity ◽

Cell Line ◽

Cell Lines ◽

Colorimetric Assay ◽

Ethanolic Extract ◽

T47d Cell ◽

Ic50 Value ◽

T47d Cell Line

Background: Several studies focused on phytochemical as agents of cancer prevention and co-chemotherapy. One of Indonesian plant which has edible fruit but it hasn’t been completely explored is Medinilla speciosa (Reinw.ex Bl.). Objective : The aim of this study is to examine the cytotoxic activity (IC50 value) of Medinilla speciosa (Reinw.ex Bl.) fruit ethanolic extract. Methods : Medinilla speciosa (Reinw.ex Bl.) fruit ethanolic extract was used in this study. The cytotoxic activity was investigated in vitro on human breast cancer T47D cell-line. The cells viability were assessed using MTT colorimetric assay. Breast cancer T47D cell lines was treated with fruit ethanolic extract (10, 25, 50, 100, 250, 500 and 1000 µg/ml) for 24 hour of incubation. This study also identified phytochemical compound of the fruit with thin layer chromatography (TLC). Results: The result showed that ethanolic extract of Medinilla speciosa (Reinw.ex Bl.) has moderate cytotoxicity on breast cancer T47D cell line with IC50 value of 614.50 µg/ml and yield the decrease of cell viability at higher concentration. Medinilla speciosa fruit can not be used as anticancer agent but chemoprevention agent. Phytochemical test showed that the fruit extract contain flavonoid and saponin compound. Conclusion: Ethanolic extract of Medinilla speciosa fruit exhibited moderate cytotocicity on breast cancer T47D cell lines with IC50 value was 614,50 µg/ml thus it can be used as chemopreventioan agent.

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