scholarly journals Chloroform fraction of ethanolic extract of Elephantopus scaber Linn. increase the p53 expression on human breast cancer (T47D) cell line

Pharmaciana ◽  
2017 ◽  
Vol 7 (2) ◽  
pp. 141 ◽  
Author(s):  
Nurkhasanah Mahfudh ◽  
Nanik Sulistyani ◽  
Lukman Mahdi
Keyword(s):  
Breast Cancer ◽  
Cell Line ◽  
Human Breast Cancer ◽  
Ethanolic Extract ◽  
T47d Cell ◽  
Chloroform Fraction ◽  
Human Breast ◽  
P53 Expression ◽  
T47d Cell Line ◽  
Elephantopus Scaber

Synthesis and cytotoxicity of some biurets against human breast cancer T47D cell line

2010 ◽  
Vol 20 (19) ◽  
pp. 5772-5775 ◽  
Author(s):  
Shamileh Fouladdel ◽  
Ali Khalaj ◽  
Neda Adibpour ◽  
Ebrahim Azizi
Keyword(s):  
Breast Cancer ◽  
Cell Line ◽  
Human Breast Cancer ◽  
T47d Cell ◽  
Human Breast ◽  
T47d Cell Line

scholarly journals Apoptosis induction on human breast cancer T47D cell line by extracts of Ancorina sp.

F1000Research ◽  
2019 ◽  
Vol 8 ◽  
pp. 168
Author(s):  
Woro Anindito Sri Tunjung ◽  
Puspa Restu Sayekti
Keyword(s):  
Breast Cancer ◽  
Cell Line ◽  
Human Breast Cancer ◽  
Caspase 3 ◽  
Cancer Cell Line ◽  
T47d Cell ◽  
High Efficacy ◽  
Human Breast ◽  
Natural Medicine ◽  
T47d Cell Line

Background: Breast cancer is the second leading cause of death in women. Alternative medicine with high efficacy is needed for breast cancer treatments, for example induction of apoptosis using natural products. It has been found that many natural apoptosis-inducing compounds are isolated from marine sponge. The objective of this study is to analyze the ability of extracts of the sponge Ancorina sp. to induce apoptosis on human breast cancer T47D cell line and find out its mechanism. Methods: T47D cells were treated with crude extracts of methanol, dichloromethane:methanol (1:1) and dichloromethane Ancorina sp. for 24 h, and doxorubicin was used as a positive control. Methods used for this study were MTT assay to examine cell viability and determine IC50 of the three extracts, while the percentage of apoptosis and caspase-3 were investigated by flow cytometry. Results: IC50 values of methanol, dichloromethane:methanol (1:1), and dichloromethane extract were 84.25, 121.45, and 99.85μg/mL respectively. The percentages of apoptotic cells after treatment with methanol, dichloromethane:methanol (1:1), and dichloromethane extracts were 88.68, 27.54 and 53.63% respectively, whereas the percentage of caspase-3 was 77.87, 12.66 and 12.97%, respectively. Conclusions: These results revealed that all extracts of Ancorina sp. have strong or moderate cytotoxicity and have the ability to induce apoptosis on T47D human breast cancer cell line. However, methanol crude extract has high efficacy to induce apoptosis through caspase-3 activation compared to the other extracts. Hence methanol extract warrants further investigation as a natural medicine for human breast cancer.

scholarly journals Anticancer effect of ethanol Lycium barbarum (Goji berry) extract on human breast cancer T47D cell line

2015 ◽  
Vol 30 (17) ◽  
pp. 1993-1996 ◽  
Author(s):  
Anna Wawruszak ◽  
Arkadiusz Czerwonka ◽  
Karolina Okła ◽  
Wojciech Rzeski
Keyword(s):  
Breast Cancer ◽  
Cell Line ◽  
Human Breast Cancer ◽  
T47d Cell ◽  
Human Breast ◽  
Anticancer Effect ◽  
Lycium Barbarum ◽  
Goji Berry ◽  
T47d Cell Line

scholarly journals Apoptosis induction on human breast cancer T47D cell line by extracts of Ancorina sp.

F1000Research ◽  
2019 ◽  
Vol 8 ◽  
pp. 168
Author(s):  
Woro Anindito Sri Tunjung ◽  
Puspa Restu Sayekti
Keyword(s):  
Breast Cancer ◽  
Cell Line ◽  
Human Breast Cancer ◽  
Caspase 3 ◽  
Cancer Cell Line ◽  
T47d Cell ◽  
High Efficacy ◽  
Human Breast ◽  
Natural Medicine ◽  
T47d Cell Line

Background: Breast cancer is the second leading cause of death in women. Alternative medicine with high efficacy is needed for breast cancer treatments, for example induction of apoptosis using natural products. It has been found that many natural apoptosis-inducing compounds are isolated from marine sponge. The objective of this study is to analyze the ability of extracts of the sponge Ancorina sp. to induce apoptosis on human breast cancer T47D cell line and find out its mechanism. Methods: T47D cells were treated with crude extracts of methanol, dichloromethane:methanol (1:1) and dichloromethane Ancorina sp. for 24 h, and doxorubicin was used as a positive control. Methods used for this study were MTT assay to examine cell viability and determine IC50 of the three extracts, while the percentage of apoptosis and caspase-3 were investigated by flow cytometry. Results: IC50 values of methanol, dichloromethane:methanol (1:1), and dichloromethane extract were 84.25, 121.45, and 99.85μg/mL respectively. The percentages of apoptotic cells after treatment with methanol, dichloromethane:methanol (1:1), and dichloromethane extracts were 88.68, 27.54 and 53.63% respectively, whereas the percentage of caspase-3 was 77.87, 12.66 and 12.97%, respectively. Conclusions: These results revealed that all extracts of Ancorina sp. have strong or moderate cytotoxicity and have the ability to induce apoptosis on T47D human breast cancer cell line. However, methanol crude extract has high efficacy to induce apoptosis through caspase-3 activation compared to the other extracts. Hence methanol extract warrants further investigation as a natural medicine for human breast cancer.

scholarly journals Cytotoxicity Assay From Fractions of Hedyotis corymbosa Extract Against Breast Cancer Cell Line T47D

2011 ◽  
Vol 2 (1) ◽  
pp. 182
Author(s):  
Rina Andriyani ◽  
Chandra Risdian ◽  
Zalinar Udin
Keyword(s):  
Breast Cancer ◽  
Herbal Medicine ◽  
Cell Line ◽  
Methylene Chloride ◽  
Cancer Cell Line ◽  
Ethanolic Extract ◽  
T47d Cell ◽  
Methylene Chloride Extract ◽  
T47d Cell Line ◽  
Cancer Medicine

Drug discovery for cancer medication is the most important effort that researcher do at this time. Indonesia bio diversities have possibility as a cancer medicine sources. Finding a herbal medicine for cancer treatment is a first step to find a right cancer medicine in the future. This research has already completed for the earlier another research. Some fractions of Hedyotis corymbosa extract has been analyzed using Sulforhodamine B method with UV wavelength 515 nm against T47D cell line, a human breast cancer. There are Hexane extract, Methylene chloride extract and Ethyl acetate extract, and give inhibitory concentration 50 (IC50)  of 33.45 µg/ml, 54.59 µg/ml and 52.58 µg/ml, respectively. Ethanolic extract, itself has IC50 of 61.57 µg/ml, whereas IC50 value of Cisplatinum is  9.63 µg/ml. There is a difference between the ethanolic extracts with the other fraction.Keywords: breast cancer, herbal medicine, T47D, Hedyotis corymbosa

scholarly journals Effect of ethanolic extract of Reissantia indica on the human breast cancer cell line (MCF-7)

2018 ◽  
Vol 7 (1) ◽  
pp. 10-12
Author(s):  
Gayathri P ◽  
◽  
Dhana Sekar V ◽  
Sudhakar K ◽  
Sangeetha M ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Line ◽  
Plant Extract ◽  
Human Breast Cancer ◽  
Cancer Cell Line ◽  
Ethanolic Extract ◽  
Human Breast Cancer Cell ◽  
Human Breast ◽  
Plant Parts ◽  
Mcf 7

Reissantia indica belongs to the family Celastraceae, is a native shrub to Maharashtra and Bengal, India. Its distinct plant parts are said to possess different activities, natives of Maharashtra and Bengal are using the plant parts for various purposes. In this experiment, the effect of ethanolic extract to destroy the cancer cells was studied. Study was carried on with human breast cancer cell line (MCF-7). Preparation of cell line was done using the reagents under standard protocol. Effect of plant extract on the cancer cells were recorded at two different timings (24 & 48 Hrs) respectively. Various doses of plant extract and standard were taken and introduced into cell line. Using 96 well plates the cells were seeded and studied for the viability. From the tabulated records, it clearly shows us the dose dependent response for the inhibition of cells. The maximum concentration of the plant extract 100µg/ml was found to be effective

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