A quantitative prediction of the sensitizing potentials by measuring tryptophan fluorescence

2011 ◽  
Vol 205 ◽  
pp. S263
Author(s):  
T. Kawamoto ◽  
H. Yu ◽  
T. Pham ◽  
M. Tanaka ◽  
D. Askew ◽  
...  
Keyword(s):  
Tryptophan Fluorescence ◽  
Quantitative Prediction

Calculating Energy Release Rate as a Function of Crack Length Using a Multiple-Step Crack Closure Technique in Tire Finite Element Models

2018 ◽  
Vol 46 (3) ◽  
pp. 130-152
Author(s):  
Dennis S. Kelliher
Keyword(s):  
Finite Element ◽  
Energy Release Rate ◽  
Crack Length ◽  
Energy Release ◽  
Crack Closure ◽  
Release Rate ◽  
Fatigue Behavior ◽  
Three Dimensions ◽  
Quantitative Prediction ◽  
Closure Technique

ABSTRACT When performing predictive durability analyses on tires using finite element methods, it is generally recognized that energy release rate (ERR) is the best measure by which to characterize the fatigue behavior of rubber. By addressing actual cracks in a simulation geometry, ERR provides a more appropriate durability criterion than the strain energy density (SED) of geometries without cracks. If determined as a function of crack length and loading history, and augmented with material crack growth properties, ERR allows for a quantitative prediction of fatigue life. Complications arise, however, from extra steps required to implement the calculation of ERR within the analysis process. This article presents an overview and some details of a method to perform such analyses. The method involves a preprocessing step that automates the creation of a ribbon crack within an axisymmetric-geometry finite element model at a predetermined location. After inflating and expanding to three dimensions to fully load the tire against a surface, full ribbon sections of the crack are then incrementally closed through multiple solution steps, finally achieving complete closure. A postprocessing step is developed to determine ERR as a function of crack length from this enforced crack closure technique. This includes an innovative approach to calculating ERR as the crack length approaches zero.

Specific features of the temperature dependence of tryptophan fluorescence lifetime in the temperature range of −170–20 °C

2020 ◽  
Vol 393 ◽  
pp. 112435
Author(s):  
Peter P. Knox ◽  
Vladimir V. Gorokhov ◽  
Boris N. Korvatovsky ◽  
Nadezhda P. Grishanova ◽  
Sergey N. Goryachev ◽  
...  
Keyword(s):  
Temperature Dependence ◽  
Fluorescence Lifetime ◽  
Tryptophan Fluorescence ◽  
Temperature Range

Quantitative prediction of flow dynamics and mechanical retention of surface-altered red blood cells through a splenic slit

2021 ◽  
Vol 33 (5) ◽  
pp. 051902
Author(s):  
Xiaojing Qi ◽  
Shuo Wang ◽  
Shuhao Ma ◽  
Keqin Han ◽  
Xuejin Li
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Flow Dynamics ◽  
Quantitative Prediction

scholarly journals A predictable smoothing evolution model for computer-controlled polishing

2020 ◽  
Vol 16 (1) ◽  
Author(s):  
Jing Hou ◽  
Pengli Lei ◽  
Shiwei Liu ◽  
Xianhua Chen ◽  
Jian Wang ◽  
...  
Keyword(s):  
Evolution Model ◽  
Quantitative Prediction ◽  
Theoretical Support ◽  
Model Combining ◽  
Process Guidance ◽  
Dependent Model ◽  
Computer Controlled Optical Surfacing ◽  
Computer Controlled ◽  
Time Dependent Model ◽  
Selection Of

AbstractQuantitative prediction of the smoothing of mid-spatial frequency errors (MSFE) is urgently needed to realize process guidance for computer controlled optical surfacing (CCOS) rather than a qualitative analysis of the processing results. Consequently, a predictable time-dependent model combining process parameters and an error decreasing factor (EDF) were presented in this paper. The basic smoothing theory, solution method and modification of this model were expounded separately and verified by experiments. The experimental results show that the theoretical predicted curve agrees well with the actual smoothing effect. The smoothing evolution model provides certain theoretical support and guidance for the quantitative prediction and parameter selection of the smoothing of MSFE.

Quantitative prediction of a functional ingredient in apple using Raman spectroscopy and multivariate calibration analysis

2021 ◽  
Vol 127 (6) ◽  
Author(s):  
Shinsaku Tsuyama ◽  
Akinori Taketani ◽  
Takeharu Murakami ◽  
Michio Sakashita ◽  
Saki Miyajima ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Multivariate Calibration ◽  
Quantitative Prediction ◽  
Functional Ingredient

scholarly journals Identification and functional analysis of cadmium-binding protein in the visceral mass of Crassostrea gigas

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Zehua Zheng ◽  
Kazuhiro Kawakami ◽  
Dingkun Zhang ◽  
Lumi Negishi ◽  
Mohamed Abomosallam ◽  
...  
Keyword(s):  
Crassostrea Gigas ◽  
Metal Ion ◽  
Defense Mechanisms ◽  
Three Dimensional ◽  
Tryptophan Fluorescence ◽  
Water Soluble ◽  
Titration Calorimetry ◽  
Visceral Mass ◽  
The Pacific ◽  
Liquid Chromatography Tandem Mass

AbstractThe Pacific oyster, Crassostrea gigas, is a traditional food worldwide. The soft body of the oyster can easily accumulate heavy metals such as cadmium (Cd). To clarify the molecular mechanism of Cd accumulation in the viscera of C. gigas, we identified Cd-binding proteins. 5,10,15,20-Tetraphenyl-21H,23H-porphinetetrasulfonic acid, disulfuric acid, tetrahydrate, and Cd-binding competition experiments using immobilized metal ion affinity chromatography revealed the binding of water-soluble high molecular weight proteins to Cd, including C. gigas protein disulfide isomerase (cgPDI). Liquid chromatography–tandem mass spectrometry (LC–MS/MS) analyses revealed two CGHC motifs in cgPDI. The binding between Cd and rcgPDI was confirmed through a Cd-binding experiment using the TPPS method. Isothermal titration calorimetry (ITC) revealed the binding of two Cd ions to one molecule of rcgPDI. Circular dichroism (CD) spectrum and tryptophan fluorescence analyses demonstrated that the rcgPDI bound to Cd. The binding markedly changed the two-dimensional or three-dimensional structures. The activity of rcgPDI measured by a PDI Activity Assay Kit was more affected by the addition of Cd than by human PDI. Immunological analyses indicated that C. gigas contained cgPDI at a concentration of 1.0 nmol/g (viscera wet weight). The combination of ITC and quantification results revealed that Cd-binding to cgPDI accounted for 20% of the total bound Cd in the visceral mass. The findings provide new insights into the defense mechanisms of invertebrates against Cd.

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