scholarly journals Identification and functional analysis of cadmium-binding protein in the visceral mass of Crassostrea gigas

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Zehua Zheng ◽  
Kazuhiro Kawakami ◽  
Dingkun Zhang ◽  
Lumi Negishi ◽  
Mohamed Abomosallam ◽  
...  
Keyword(s):  
Crassostrea Gigas ◽  
Metal Ion ◽  
Defense Mechanisms ◽  
Three Dimensional ◽  
Tryptophan Fluorescence ◽  
Water Soluble ◽  
Titration Calorimetry ◽  
Visceral Mass ◽  
The Pacific ◽  
Liquid Chromatography Tandem Mass

AbstractThe Pacific oyster, Crassostrea gigas, is a traditional food worldwide. The soft body of the oyster can easily accumulate heavy metals such as cadmium (Cd). To clarify the molecular mechanism of Cd accumulation in the viscera of C. gigas, we identified Cd-binding proteins. 5,10,15,20-Tetraphenyl-21H,23H-porphinetetrasulfonic acid, disulfuric acid, tetrahydrate, and Cd-binding competition experiments using immobilized metal ion affinity chromatography revealed the binding of water-soluble high molecular weight proteins to Cd, including C. gigas protein disulfide isomerase (cgPDI). Liquid chromatography–tandem mass spectrometry (LC–MS/MS) analyses revealed two CGHC motifs in cgPDI. The binding between Cd and rcgPDI was confirmed through a Cd-binding experiment using the TPPS method. Isothermal titration calorimetry (ITC) revealed the binding of two Cd ions to one molecule of rcgPDI. Circular dichroism (CD) spectrum and tryptophan fluorescence analyses demonstrated that the rcgPDI bound to Cd. The binding markedly changed the two-dimensional or three-dimensional structures. The activity of rcgPDI measured by a PDI Activity Assay Kit was more affected by the addition of Cd than by human PDI. Immunological analyses indicated that C. gigas contained cgPDI at a concentration of 1.0 nmol/g (viscera wet weight). The combination of ITC and quantification results revealed that Cd-binding to cgPDI accounted for 20% of the total bound Cd in the visceral mass. The findings provide new insights into the defense mechanisms of invertebrates against Cd.

NUCLEAR MICROPROBE AND RAMAN INVESTIGATION OF THE CHEMISTRY OF THE SHELL OF THE PACIFIC OYSTER, CRASSOSTREA GIGAS

1999 ◽  
Vol 09 (03n04) ◽  
pp. 345-352 ◽  
Author(s):  
A. MARKWITZ ◽  
R. W. GAULDIE ◽  
J. PITHIE ◽  
S. K. SHARMA ◽  
D. J. JAMIESON
Keyword(s):  
Calcium Carbonate ◽  
Crassostrea Gigas ◽  
Metal Ion ◽  
Pacific Oyster ◽  
Ion Beam ◽  
Nuclear Microprobe ◽  
Nuclear Microscopy ◽  
The Pacific ◽  
Significant Difference ◽  
Mineral Constituent

High-resolution nuclear microscopy was used to study the layered structure in the shell of the pacific oyster, Crassostrea gigas. In cross section, the layers appear as opaque white zones and clearer translucent zones. Raman spectroscopy indicates that the zones consist of alternating layers of the aragonite and calcite morphs of calcium carbonate, the mineral constituent of the shell. The chemistry of the shell varies from individual to individual but generally the predominant metal ion is Ca , with varying amounts of Si , Cl , Cr , Mn , Fe , Zn , Sb , Ni , Fe , As and Sr . Two dimensional maps of these major, minor and trace elements were measured in many shells with nuclear microscopy to identify the patterns of Zn and Sr deposition reflecting the calcite and aragonite layers. The significant difference in the patterns identified by ion beam analyses are possibly a result of isostructural exclusion of these metal ions between the different aragonite and calcite polymorphic forms of calcium carbonate.

Combined effects of pollutants and salinity on embryo-larval development of the Pacific oyster, Crassostrea gigas

2016 ◽  
Vol 113 ◽  
pp. 31-38 ◽  
Author(s):  
Perrine Gamain ◽  
Patrice Gonzalez ◽  
Jérôme Cachot ◽  
Patrick Pardon ◽  
Nathalie Tapie ◽  
...  
Keyword(s):  
Larval Development ◽  
Crassostrea Gigas ◽  
Pacific Oyster ◽  
Combined Effects ◽  
The Pacific

Differentially methylated gene regions between resistant and susceptible heat-phenotypes of the Pacific oyster Crassostrea gigas

Aquaculture ◽  
2021 ◽  
pp. 736923
Author(s):  
Roberto Arredondo-Espinoza ◽  
Ana M. Ibarra ◽  
Steven B. Roberts ◽  
Maria Teresa Sicard-Gonzalez ◽  
Cristina Escobedo-Fregoso
Keyword(s):  
Crassostrea Gigas ◽  
Pacific Oyster ◽  
The Pacific ◽  
Differentially Methylated Gene

scholarly journals Anchoring Water Soluble Phosphotungstic Acid by Hybrid Fillers to Construct Three-Dimensional Proton Transport Networks

Membranes ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 536
Author(s):  
Shaojian He ◽  
Zhongrui Lu ◽  
Wenxu Dai ◽  
Kangning Yang ◽  
Yang Xue ◽  
...  
Keyword(s):  
Relative Humidity ◽  
Composite Membrane ◽  
Proton Transport ◽  
Phosphotungstic Acid ◽  
Three Dimensional ◽  
Water Soluble ◽  
Hydroxyl Groups ◽  
Transport Networks ◽  
Hybrid Fillers ◽  
Speek Membrane

Phosphotungstic acid (HPW)-filled composite proton exchange membranes possess high proton conductivity under low relative humidity (RH). However, the leaching of HPW limits their wide application. Herein, we propose a novel approach for anchoring water soluble phosphotungstic acid (HPW) by polydopamine (PDA) coated graphene oxide and halloysite nanotubes (DGO and DHNTs) in order to construct hybrid three-dimensional proton transport networks in a sulfonated poly(ether ether ketone) (SPEEK) membrane. The introduction of PDA on the surfaces of the hybrid fillers could provide hydroxyl groups and secondary amine groups to anchor HPW, resulting in the uniform dispersion of HPW in the SPEEK matrix. The SPEEK/DGO/DHNTs/HPW (90/5/5/60) composite membrane exhibited higher water uptake and much better conductivity than the SPEEK membrane at low relative humidity. The best conductivity reached wass 0.062 S cm−1 for the composite membrane, which is quite stable during the water immersion test.

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