Effects of ticks on community assemblages of ectoparasites in deer mice

1996 ◽

Vol 54 ◽

pp. 30-31

Author(s):

J. T. Ellzey ◽

D. Borunda ◽

B. P. Stewart

Keyword(s):

South Carolina ◽

Adult Male ◽

Uranyl Acetate ◽

Model System ◽

Deer Mice ◽

En Bloc ◽

Genetic Stock ◽

Hepes Buffer ◽

Transmission Electron ◽

Color Scanner

Genetically alcohol deficient deer mice (ADHN/ADHN) (obtained from the Peromyscus Genetic Stock Center, Univ. of South Carolina) lack hepatic cytosolic alcohol dehydrogenase. In order to determine if these deer mice would provide a model system for an ultrastructural study of the effects of ethanol on hepatocyte organelles, 75 micrographs of ADH+ adult male deer mice (n=5) were compared with 75 micrographs of ADH− adult male deer mice (n=5). A morphometric analysis of mitochondrial and peroxisomal parameters was undertaken.The livers were perfused with 0.1M HEPES buffer followed by 0.25% glutaraldehyde and 2% sucrose in 0.1M HEPES buffer (4C), removed, weighed and fixed by immersion in 2.5% glutaraldehyde in 0.1M HEPES buffer, pH 7.4, followed by a 3,3’ diaminobenzidine (DAB) incubation, postfixation with 2% OsO4, en bloc staining with 1% uranyl acetate in 0.025M maleate-NaOH buffer, dehydrated, embedded in Poly/Bed 812-BDMA epon resin, sectioned and poststained with uranyl acetate and lead citrate. Photographs were taken on a Zeiss EM-10 transmission electron microscope, scanned with a Howtek personal color scanner, analyzed with OPTIMAS 4.02 software on a Gateway2000 4DX2-66V personal computer and stored in Excel 4.0.

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Identification of the pulmonary syndrome hantavirus by direct and colloidal gold immune Electron Microscopy

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100169109 ◽

1994 ◽

Vol 52 ◽

pp. 274-275

Author(s):

C. D. Humphrey ◽

C.S. Goldsmith ◽

L. Elliott ◽

S.R. Zaki

Keyword(s):

Electron Microscopy ◽

United States ◽

Monoclonal Antibody ◽

Colloidal Gold ◽

Phosphotungstic Acid ◽

Uranyl Acetate ◽

Hantavirus Pulmonary Syndrome ◽

Deer Mice ◽

Immune Electron Microscopy ◽

Negative Stain

An outbreak of unexplained acute pulmonary syndrome with high fatality was recognized in the spring of 1993 in the southwestern United States. The cause of the illness was quickly identified serologically and genetically as a hantavirus and the disease was named hantavirus pulmonary syndrome (HPS). Recently, the virus was isolated from deer mice which had been trapped near the homes of HPS patients, and cultivated in Vero E6 cells. We identified the cultivated virus by negative-stain direct and colloidal gold immune electron microscopy (EM).Virus was extracted, clarified, and concentrated from unfixed and 0.25% glutaraldehyde fixed supernatant fluids of infected Vero E6 cells by a procedure described previously. Concentrated virus suspensions tested by direct EM were applied to glow-discharge treated formvar-carbon filmed grids, blotted, and stained with 0.5% uranyl acetate (UA) or with 2% phosphotungstic acid (PTA) pH 6.5. Virus suspensions for immune colloidal gold identification were adsorbed similarly to filmed grids but incubated for 1 hr on drops of 1:50 diluted monoclonal antibody to Prospect Hill virus nucleoprotein or with 1:50 diluted sera from HPS virus infected deer mice.

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Native mice in a novel ecosystem: Morphology, behavior, and sociology predict the role of prairie deer mice in agriculture

10.31274/etd-180810-620 ◽

2014 ◽

Author(s):

John Watson Doudna

Keyword(s):

Deer Mice ◽

Novel Ecosystem

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Faculty Opinions recommendation of The molecular basis of high-altitude adaptation in deer mice.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1079774.532691 ◽

2007 ◽

Author(s):

Ary Hoffmann

Keyword(s):

High Altitude ◽

Molecular Basis ◽

Deer Mice ◽

High Altitude Adaptation ◽

Altitude Adaptation

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The Role of Isolation in the Formation of a Narrowly Localized Race of Deer-Mice (Peromyscus)

The American Naturalist ◽

10.1086/279595 ◽

1917 ◽

Vol 51 (603) ◽

pp. 173-185 ◽

Cited By ~ 9

Author(s):

F. B. Sumner

Keyword(s):

Deer Mice

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Dehydrogenase-dependent Ethanol Metabolism in Deer Mice (Peromyscus maniculatus) Lacking Cytosolic Alcohol Dehydrogenase

Journal of Biological Chemistry ◽

10.1016/s0021-9258(18)83588-8 ◽

1989 ◽

Vol 264 (10) ◽

pp. 5593-5597

Author(s):

C Norsten ◽

T Cronholm ◽

G Ekström ◽

J A Handler ◽

R G Thurman ◽

...

Keyword(s):

Alcohol Dehydrogenase ◽

Peromyscus Maniculatus ◽

Ethanol Metabolism ◽

Deer Mice

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SARS-CoV-2 infection and transmission in the North American deer mouse

Nature Communications ◽

10.1038/s41467-021-23848-9 ◽

2021 ◽

Vol 12 (1) ◽

Author(s):

Bryan D. Griffin ◽

Mable Chan ◽

Nikesh Tailor ◽

Emelissa J. Mendoza ◽

Anders Leung ◽

...

Keyword(s):

Respiratory Tract ◽

North American ◽

Direct Contact ◽

Lower Respiratory Tract ◽

Infectious Virus ◽

Deer Mouse ◽

Deer Mice ◽

The North ◽

Wild Deer ◽

Theoretical Risk

AbstractWidespread circulation of SARS-CoV-2 in humans raises the theoretical risk of reverse zoonosis events with wildlife, reintroductions of SARS-CoV-2 into permissive nondomesticated animals. Here we report that North American deer mice (Peromyscus maniculatus) are susceptible to SARS-CoV-2 infection following intranasal exposure to a human isolate, resulting in viral replication in the upper and lower respiratory tract with little or no signs of disease. Further, shed infectious virus is detectable in nasal washes, oropharyngeal and rectal swabs, and viral RNA is detectable in feces and occasionally urine. We further show that deer mice are capable of transmitting SARS-CoV-2 to naïve deer mice through direct contact. The extent to which these observations may translate to wild deer mouse populations remains unclear, and the risk of reverse zoonosis and/or the potential for the establishment of Peromyscus rodents as a North American reservoir for SARS-CoV-2 remains unknown.

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