Comparative evaluation of positive tests to Mycobacterium avium subsp. paratuberculosis in clinically healthy sheep and goats in South-West Greece using molecular techniques, serology, and culture

2007 ◽  
Vol 174 (2) ◽  
pp. 337-343 ◽  
Author(s):  
John Ikonomopoulos ◽  
Christos Balaskas ◽  
Bagia Kantzoura ◽  
Eirini Fragiadaki ◽  
Ivo Pavlik ◽  
...  
Keyword(s):  
Mycobacterium Avium ◽  
Comparative Evaluation ◽  
Molecular Techniques ◽  
Mycobacterium Avium Subsp ◽  
Sheep And Goats ◽  
South West ◽  
Healthy Sheep ◽  
Mycobacterium Avium Subsp Paratuberculosis

scholarly journals Detection of Mycobacterium avium subsp. paratuberculosis in liver of slaughtered cattle, sheep and goats by PCR and culture

2021 ◽  
Vol 72 (1) ◽  
pp. 2683
Author(s):  
M ZAREI ◽  
S TAJBAKHSH ◽  
M GHORBANPOUR ◽  
A YOUSEFVAND ◽  
S RAHIMI ◽  
...  
Keyword(s):  
Mycobacterium Avium ◽  
Disease Status ◽  
Johne's Disease ◽  
Johne’S Disease ◽  
Mycobacterium Avium Subsp ◽  
Health Concern ◽  
Sheep And Goats ◽  
Mycobacterium Avium Subsp Paratuberculosis ◽  
Positive Results ◽  
Potential Public Health

The presence of Mycobacterium avium subsp. paratuberculosis (MAP) in tissues other than intestines and associated lymph nodes is a potential public health concern. Therefore, the presence of MAP in the liver of slaughtered cattle, sheep and goats with unknown Johne’s disease status was evaluated. Liver samples were collected randomly from 470 mature animals including 200 cattle, 135 sheep and 135 goats. The existence of MAP DNA from obtained liver samples was determined using PCR, thereafter PCR-confirmed samples were cultured. Based on liver PCR results, IS900 genes were detected in 11/200 (5.5%), 3/135 (2.2%), 2/135 (1.48%) liver samples of cattle, sheep and goats, respectively. In cattle, sheep and goats, 6 (54.5%), 1 (33.3%) and 1 (50%) PCR-positive samples showed positive results in culture, respectively. Our results revealed that MAP can be detected and cultured from the liver of slaughtered cattle, sheep and goats with unknown Johne’s disease status. Hence, given concerns about a potential zoonotic role for MAP, there is a crucial need to detect animals with MAP disseminated infection in the liver before slaughter.

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