Immunogenicity and efficacy of three recombinant subunit Pasteurella multocida toxin vaccines against progressive atrophic rhinitis in pigs

Vaccine ◽  
2006 ◽  
Vol 24 (1) ◽  
pp. 27-35 ◽  
Author(s):  
Chih-Ming Liao ◽  
Chienjin Huang ◽  
Shih-Ling Hsuan ◽  
Zeng-Weng Chen ◽  
Wei-Cheng Lee ◽  
...  
Keyword(s):  
Pasteurella Multocida ◽  
Atrophic Rhinitis ◽  
Pasteurella Multocida Toxin ◽  
Progressive Atrophic Rhinitis

scholarly journals Involvement of Osteocytes in the Action of Pasteurella multocida Toxin

Toxins ◽  
2018 ◽  
Vol 10 (8) ◽  
pp. 328 ◽  
Author(s):  
Hannah Heni ◽  
Julia Ebner ◽  
Gudula Schmidt ◽  
Klaus Aktories ◽  
Joachim Orth
Keyword(s):  
G Proteins ◽  
Pasteurella Multocida ◽  
Fiber Formation ◽  
Atrophic Rhinitis ◽  
Target Cells ◽  
Heterotrimeric G Proteins ◽  
Culture Model ◽  
Tnf Α ◽  
Pasteurella Multocida Toxin ◽  
Progressive Atrophic Rhinitis

Pasteurella multocida toxin (PMT) causes progressive atrophic rhinitis with severe turbinate bone degradation in pigs. It has been reported that the toxin deamidates and activates heterotrimeric G proteins, resulting in increased differentiation of osteoclasts and blockade of osteoblast differentiation. So far, the action of PMT on osteocytes, which is the most abundant cell type in bone tissue, is not known. In MLO-Y4 osteocytes, PMT deamidated heterotrimeric G proteins, resulting in loss of osteocyte dendritic processes, stress fiber formation, cell spreading and activation of RhoC but not of RhoA. Moreover, the toxin caused processing of membrane-bound receptor activator of NF-κB ligand (RANKL) to release soluble RANKL and enhanced the secretion of osteoclastogenic TNF-α. In a co-culture model of osteocytes and bone marrow cells, PMT-induced osteoclastogenesis was largely increased as compared to the mono-culture model. The enhancement of osteoclastogenesis observed in the co-culture was blocked by sequestering RANKL with osteoprotegerin and by an antibody against TNF-α indicating involvement of release of the osteoclastogenic factors from osteocytes. Data support the crucial role of osteocytes in bone metabolism and osteoclastogenesis and identify osteocytes as important target cells of PMT in progressive atrophic rhinitis.

Eignung des Tiergesundheitsdienstprogramms zur Überwachung und Bekämpfung der progressiven Rhinitis atrophicans

2009 ◽  
Vol 37 (06) ◽  
pp. 379-385
Author(s):  
C. Lang ◽  
M. Huber ◽  
A. Griessler ◽  
M. F. De Jong ◽  
M. Schuh ◽  
...  
Keyword(s):  
Pasteurella Multocida ◽  
Atrophic Rhinitis ◽  
Klinische Relevanz ◽  
Pasteurella Multocida Toxin

Zusammenfassung Gegenstand und Ziel: Im Zeitraum von 1999 bis 2005 erfolgte eine Statuserhebung über das Vorkommen der progressiven Rhinitis atrophicans (PAR) in 17 oberösterreichischen Schweinevermehrerbetrieben, um in weiterer Folge eine PAR-Freiheit und Zertifizierung zu erreichen und somit die Eignung des Tiergesundheitsdienstprogramms für diesen Zweck zu überprüfen. Material und Methoden: Entsprechend dem Tiergesundheitsdienstprogramm wurden 11 045 Nasen- und Tonsillartupferproben entnommen und mittels PCR auf Pasteurella-multocida- Toxin (PMT) untersucht. Ein Jahr nach Einstellung der ART-(Atrophic- rhinitis-toxoid-)Vakzinierung wurden 2004 und 2005 zusätzlich 179 Blutproben mittels ELISA auf Antikörper gegen PMT untersucht. Ergebnisse: 84 (0,76%) der Nasen- und Tonsillartupferproben waren PMT-positiv. Bei 43 (24,02%) Seren ergab sich ein positiver serologischer Befund, es lagen jedoch keine klinischen Symptome einer PAR vor. Bereits im Jahr 2003 war in 14 Betrieben PMT in Nasen- und Tonsillartupferproben nicht mehr nachweisbar. In 16 Betrieben gelang bis dato die Eradikation des Erregers, lediglich ein Betrieb musste aufgrund der Nichteinhaltung der Vorschriften auf den Status PAR-frei“ verzichten. Schlussfolgerung: Die Studie belegt, dass sich das empfohlene Programm, das auf einem Test-and-Removal-“(T&R-)Prinzip basiert, zur stufenweisen Bekämpfung der PAR ohne komplette Bestandsmerzung eignet. Klinische Relevanz: Das Tiergesundheitsdienstprogramm zur Überwachung und Bekämpfung der progressiven Rhinitis atrophicans konnte im Laufe dieser Studie etabliert werden und wird mittlerweile österreichweit durchgeführt.

scholarly journals In Vivo Targets of Pasteurella Multocida Toxin

2020 ◽  
Vol 21 (8) ◽  
pp. 2739
Author(s):  
Arshiya Banu ◽  
Alistair J. Lax ◽  
Agamemnon E. Grigoriadis
Keyword(s):  
G Proteins ◽  
Protein Modification ◽  
Pasteurella Multocida ◽  
Term Treatment ◽  
Atrophic Rhinitis ◽  
Short Term Treatment ◽  
Experimental Conditions ◽  
Proliferation Markers ◽  
Pasteurella Multocida Toxin

Many Pasteurella multocida strains are carried as commensals, while some cause disease in animals and humans. Some type D strains cause atrophic rhinitis in pigs, where the causative agent is known to be the Pasteurella multocida toxin (PMT). PMT activates three families of G-proteins—Gq/11, G12/13, and Gi/o—leading to cellular mitogenesis and other sequelae. The effects of PMT on whole animals in vivo have been investigated previously, but only at the level of organ-specific pathogenesis. We report here the first study to screen all the organs targeted by the toxin by using the QE antibody that recognizes only PMT-modified G-proteins. Under our experimental conditions, short-term treatment of PMT is shown to have multiple in vivo targets, demonstrating G-alpha protein modification, stimulation of proliferation markers and expression of active β-catenin in a tissue- and cell-specific manner. This highlights the usefulness of PMT as an important tool for dissecting the specific roles of different G-alpha proteins in vivo.

scholarly journals Molecular Fingerprinting of Pasteurella Multocida Associated with Progressive Atrophic Rhinitis in Swine Herds

1994 ◽  
Vol 6 (4) ◽  
pp. 442-447 ◽  
Author(s):  
Ian A. Gardner ◽  
Rick Kasten ◽  
Graeme J. Eamens ◽  
Kurt P. Snipes ◽  
Randall J. Anderson
Keyword(s):  
Pasteurella Multocida ◽  
Uv Light ◽  
Type A ◽  
Atrophic Rhinitis ◽  
Capsular Type ◽  
Molecular Fingerprinting ◽  
Whole Cell ◽  
Type D ◽  
Progressive Atrophic Rhinitis ◽  
Swine Herds

Ninety-six nasal isolates of Pasteurella multocida from swine herds with progressive atrophic rhinitis were characterized by restriction endonuclease analysis (REA) of whole-cell DNA, ribotyping, and plasmid analysis. For REA, bacterial DNA was digested with SmaI and electrophoresed in 0.7% agarose, and fragments were visualized with UV light. For ribotyping, EcoRI-digested and electrophoresed restriction fragments of whole-cell DNA were transferred to nitrocellulose membranes, hybridized with γ-32P-labeled Escherichia coli ribosomal RNA, and visualized by autoradiography. Phenotypes of isolates were toxigenic capsular type D ( n = 51), nontoxigenic type D ( n = 28), nontoxigenic type A ( n = 16), and toxigenic type A ( n = 1). Plasmids of various sizes were evident in 92.2% and 17.9% of toxigenic and nontoxigenic D strains, respectively, but were absent from all type A strains. Among the 4 phenotypes, there were 17 REA profiles and 6 ribotypes. For 3 of 17 REA patterns, multiple ribotypes were evident, and several REA types were evident in 5 of 6 ribotypes. Thirty-seven isolates of toxigenic capsular type D from Australian herds were either SmaI type B or C and ribotype 2, whereas 14 toxigenic D isolates from the USA and other countries were more heterogeneous (7 REA types and 6 ribotypes). The fingerprinting results provided evidence in support of the hypothesis of a single source infection in Australia associated with the introduction of breeding pigs from overseas.

Efficacy of a novel Pasteurella multocida vaccine against progressive atrophic rhinitis of swine

Vaccine ◽  
2009 ◽  
Vol 27 (22) ◽  
pp. 2923-2929 ◽  
Author(s):  
Shih-Ling Hsuan ◽  
Chih-Ming Liao ◽  
Chienjin Huang ◽  
James R. Winton ◽  
Zeng-Weng Chen ◽  
...  
Keyword(s):  
Pasteurella Multocida ◽  
Atrophic Rhinitis ◽  
Progressive Atrophic Rhinitis
Sign in / Sign up

Export Citation Format

Share Document