scholarly journals Molecular Fingerprinting of Pasteurella Multocida Associated with Progressive Atrophic Rhinitis in Swine Herds

1994 ◽  
Vol 6 (4) ◽  
pp. 442-447 ◽  
Author(s):  
Ian A. Gardner ◽  
Rick Kasten ◽  
Graeme J. Eamens ◽  
Kurt P. Snipes ◽  
Randall J. Anderson
Keyword(s):  
Pasteurella Multocida ◽  
Uv Light ◽  
Type A ◽  
Atrophic Rhinitis ◽  
Capsular Type ◽  
Molecular Fingerprinting ◽  
Whole Cell ◽  
Type D ◽  
Progressive Atrophic Rhinitis ◽  
Swine Herds

Ninety-six nasal isolates of Pasteurella multocida from swine herds with progressive atrophic rhinitis were characterized by restriction endonuclease analysis (REA) of whole-cell DNA, ribotyping, and plasmid analysis. For REA, bacterial DNA was digested with SmaI and electrophoresed in 0.7% agarose, and fragments were visualized with UV light. For ribotyping, EcoRI-digested and electrophoresed restriction fragments of whole-cell DNA were transferred to nitrocellulose membranes, hybridized with γ-32P-labeled Escherichia coli ribosomal RNA, and visualized by autoradiography. Phenotypes of isolates were toxigenic capsular type D ( n = 51), nontoxigenic type D ( n = 28), nontoxigenic type A ( n = 16), and toxigenic type A ( n = 1). Plasmids of various sizes were evident in 92.2% and 17.9% of toxigenic and nontoxigenic D strains, respectively, but were absent from all type A strains. Among the 4 phenotypes, there were 17 REA profiles and 6 ribotypes. For 3 of 17 REA patterns, multiple ribotypes were evident, and several REA types were evident in 5 of 6 ribotypes. Thirty-seven isolates of toxigenic capsular type D from Australian herds were either SmaI type B or C and ribotype 2, whereas 14 toxigenic D isolates from the USA and other countries were more heterogeneous (7 REA types and 6 ribotypes). The fingerprinting results provided evidence in support of the hypothesis of a single source infection in Australia associated with the introduction of breeding pigs from overseas.

scholarly journals Characterization and comparison of strains of Pasteurella multocida associated with cases of progressive atrophic rhinitis and porcine pneumonia in Argentina

2019 ◽  
Vol 12 (3) ◽  
pp. 434-439
Author(s):  
Fernando A. Bessone ◽  
Maria Laura Soriano Perez ◽  
Gustavo Zielinski ◽  
Marina Dibarbora ◽  
M. B. Conde ◽  
...  
Keyword(s):  
Antibiotic Susceptibility ◽  
Pasteurella Multocida ◽  
Type A ◽  
Economic Loss ◽  
Diffusion Method ◽  
Atrophic Rhinitis ◽  
Specific Gene ◽  
Capsular Type ◽  
Progressive Atrophic Rhinitis ◽  
Susceptibility Profiles

Background: Pasteurella multocida (Pm) is the causative agent of progressive atrophic rhinitis (PAR) and pneumonic pasteurellosis (PN) in pigs. Pm is a member of the porcine respiratory complex responsible for important economic loss in the pig industry. Aim: This study aimed to characterize the Pm strains recovered from clinical cases of PN and PAR and to elucidate the antibiotic susceptibility profiles of the strains. Materials and Methods: Sixty strains were characterized molecularly by polymerase chain reaction to determine species-specific gene, capsular type (A or D), and toxin A production. The agar diffusion method was employed to evaluate antibiotic resistance profiles. Results: We found that 65% of strains belonged to capsular type A or D, and 15% of those were positive to toxA gene. The antibiotic susceptibility profiles found were sensitive in decreasing order to: Enrofloxacin, ceftiofur (CTF), ampicillin, tilmicosin (TIL), florfenicol (FFN), spectinomycin (SPC), gentamicin, oxytetracycline (OTC), and trimethoprim-sulfamethoxazole (TMS). Strains were resistant in decreasing order to: Lincomycin (LIN), tylosin (TYL), erythromycin (ERY), TMS, SPC, OTC, FFN, TIL, and CTF. Conclusion: The toxA gene was detected in many Pm isolates from pneumonic lungs. Capsule type A or D was the most frequently found among the collected isolates. LIN, TYL, and ERY are the drugs which showed higher percentages of resistant isolates.

Phenotypic and genotypic characterisation of Pasteurella multocida strains isolated from pigs in Hungary

2007 ◽  
Vol 55 (4) ◽  
pp. 425-434 ◽  
Author(s):  
Zsuzsanna Varga ◽  
Boglárka Sellyei ◽  
T. Magyar
Keyword(s):  
Multiplex Pcr ◽  
Ornithine Decarboxylase ◽  
Pasteurella Multocida ◽  
Regional Distribution ◽  
Type A ◽  
Capsule Type ◽  
Type D ◽  
Biochemical Characterisation ◽  
River Danube ◽  
Swine Herds

A total of 146 Pasteurella multocida strains isolated from swine in Hungary in the last 20 years were examined. Biochemical characterisation and PCR-based techniques were used to determine species, subspecies, biovar, capsule type and presence of the toxA gene. Eighty-seven percent of the isolates belonged to P. multocida ssp. multocida , and 98% of these had biovar 3 or were trehalose-or lactose-fermenting or ornithine decarboxylase negative variants of that. Ten percent of the strains were P. multocida ssp. septica , and within this group 80% of the strains showed sorbitol-negative biovars (5, 6 and 7). The rest of the strains (20%) were lactose positive. Only 3% of the porcine isolates were P. multocida ssp. gallicida and 3 out of the 4 strains belonged to the dulcitol-fermenting biovar 8. Using a capsule-specific multiplex PCR, 60% of the strains belonged to capsule type D, 38% to capsule type A, and only 1 isolate had capsule type F. In contrast with data published in the literature, only 3% of capsule type D isolates carried the toxA gene, while this ratio was 41% for the type A strains. A remarkable regional distribution of toxA gene positive strains was observed. All but two isolates were found in swine herds located in the Transdanubian region, separated from other parts of Hungary by the river Danube.

scholarly journals Occurrence of Genes Encoding Virulence Factors in Bordetella Bronchiseptica Strains Isolated from Infected and Healthy Pigs

2012 ◽  
Vol 56 (4) ◽  
pp. 483-487 ◽  
Author(s):  
Katarzyna Stępniewska ◽  
Iwona Markowska-Daniel
Keyword(s):  
Health Status ◽  
Virulence Factors ◽  
Bordetella Bronchiseptica ◽  
Atrophic Rhinitis ◽  
Genes Encoding ◽  
Progressive Atrophic Rhinitis ◽  
Swine Herds ◽  
Clinical Cases

Abstract The objective of the study was to determine genotypic profiles of Bordetella bronchiseptica (Bbr) strains, based on the occurrence of genes encoding virulence factors, such as flagella (fla), dermonecrotoxin (dnt), and exogenous ferric siderophore receptor (bfrZ), using PCR. 209 tested Bbr strains were obtained from Polish swine herds with different health status (with progressive atrophic rhinitis - PAR, suspected for PAR, and unknown). In total, seven different Bbr genotypes were determined. In 39.2% of Bbr isolates all three genes were present. In 41.1% of the isolates only two genes were detected. The most common genotype dnt+bfrZ-fla+ was present in 60 (28.5%) Bbr strains, 65% of them were obtained from farms with PAR. Twenty five (12%) Bbr isolates were identified as dnt-bfrZ+fla+ genotype and, as above, they were more frequently isolated from clinical cases of disease (84%). Among 31 (14.8%) strains only fla gene was evident, and in nine (4.3%) only dnt gene was present. There were no Bbr strains with bfrZ gene only. These results confirm the heterogenicity among Bbr strains.

scholarly journals Use of ELISA to Detect Toxigenic Pasteurella Multocida in Atrophic Rhinitis in Swine

1992 ◽  
Vol 4 (4) ◽  
pp. 419-422 ◽  
Author(s):  
Terry L. Bowersock ◽  
Tom Hooper ◽  
Ronald Pottenger
Keyword(s):  
Tissue Culture ◽  
Pasteurella Multocida ◽  
Atrophic Rhinitis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Cytotoxicity Assays ◽  
Highly Sensitive ◽  
Nasal Secretions ◽  
Toxigenic Strains ◽  
Swine Herds ◽  
Toxigenic Pasteurella Multocida

The use of an enzyme-linked immunosorbent assay (ELISA) as a means of detecting dermone-crotoxin-producing strains of Pasteurella multocida was investigated. The assay was evaluated as a means to identify toxigenic P. multocida isolates recovered from nasal secretions of swine with atrophic rhinitis. The sensitivity and specificity of the ELISA for detecting dermonecrotoxin-producing P. multocida strains were compared to those of mouse-inoculation and cytotoxicity assays. The ELISA was highly sensitive and more specific than animal inoculation or tissue culture assay and is thus a more effective method for screening swine herds for the presence of toxigenic strains of P. multocida. The ELISA is a rapid, effective, economical way to identify toxigenic P. multocida isolates.

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