Efficacy of a novel Pasteurella multocida vaccine against progressive atrophic rhinitis of swine

Ninety-six nasal isolates of Pasteurella multocida from swine herds with progressive atrophic rhinitis were characterized by restriction endonuclease analysis (REA) of whole-cell DNA, ribotyping, and plasmid analysis. For REA, bacterial DNA was digested with SmaI and electrophoresed in 0.7% agarose, and fragments were visualized with UV light. For ribotyping, EcoRI-digested and electrophoresed restriction fragments of whole-cell DNA were transferred to nitrocellulose membranes, hybridized with γ-32P-labeled Escherichia coli ribosomal RNA, and visualized by autoradiography. Phenotypes of isolates were toxigenic capsular type D ( n = 51), nontoxigenic type D ( n = 28), nontoxigenic type A ( n = 16), and toxigenic type A ( n = 1). Plasmids of various sizes were evident in 92.2% and 17.9% of toxigenic and nontoxigenic D strains, respectively, but were absent from all type A strains. Among the 4 phenotypes, there were 17 REA profiles and 6 ribotypes. For 3 of 17 REA patterns, multiple ribotypes were evident, and several REA types were evident in 5 of 6 ribotypes. Thirty-seven isolates of toxigenic capsular type D from Australian herds were either SmaI type B or C and ribotype 2, whereas 14 toxigenic D isolates from the USA and other countries were more heterogeneous (7 REA types and 6 ribotypes). The fingerprinting results provided evidence in support of the hypothesis of a single source infection in Australia associated with the introduction of breeding pigs from overseas.

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Demonstration that Australian Pasteurella multocida isolates from sporadic outbreaks of porcine pneumonia are non-toxigenic (toxA-) and display heterogeneous DNA restriction endonuclease profiles compared with toxigenic isolates from herds with progressive atrophic rhinitis

Journal of Medical Microbiology ◽

10.1099/00222615-47-8-679 ◽

1998 ◽

Vol 47 (8) ◽

pp. 679-688 ◽

Cited By ~ 16

Author(s):

S. P. Djordjevic ◽

G. J. Eamens ◽

H. Ha ◽

M. J. Walker ◽

J. C. Chin

Keyword(s):

Restriction Endonuclease ◽

Pasteurella Multocida ◽

Atrophic Rhinitis ◽

Dna Restriction ◽

Progressive Atrophic Rhinitis

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Characterization and comparison of strains of Pasteurella multocida associated with cases of progressive atrophic rhinitis and porcine pneumonia in Argentina

Veterinary World ◽

10.14202/vetworld.2019.434-439 ◽

2019 ◽

Vol 12 (3) ◽

pp. 434-439

Author(s):

Fernando A. Bessone ◽

Maria Laura Soriano Perez ◽

Gustavo Zielinski ◽

Marina Dibarbora ◽

M. B. Conde ◽

...

Keyword(s):

Antibiotic Susceptibility ◽

Pasteurella Multocida ◽

Type A ◽

Economic Loss ◽

Diffusion Method ◽

Atrophic Rhinitis ◽

Specific Gene ◽

Capsular Type ◽

Progressive Atrophic Rhinitis ◽

Susceptibility Profiles

Background: Pasteurella multocida (Pm) is the causative agent of progressive atrophic rhinitis (PAR) and pneumonic pasteurellosis (PN) in pigs. Pm is a member of the porcine respiratory complex responsible for important economic loss in the pig industry. Aim: This study aimed to characterize the Pm strains recovered from clinical cases of PN and PAR and to elucidate the antibiotic susceptibility profiles of the strains. Materials and Methods: Sixty strains were characterized molecularly by polymerase chain reaction to determine species-specific gene, capsular type (A or D), and toxin A production. The agar diffusion method was employed to evaluate antibiotic resistance profiles. Results: We found that 65% of strains belonged to capsular type A or D, and 15% of those were positive to toxA gene. The antibiotic susceptibility profiles found were sensitive in decreasing order to: Enrofloxacin, ceftiofur (CTF), ampicillin, tilmicosin (TIL), florfenicol (FFN), spectinomycin (SPC), gentamicin, oxytetracycline (OTC), and trimethoprim-sulfamethoxazole (TMS). Strains were resistant in decreasing order to: Lincomycin (LIN), tylosin (TYL), erythromycin (ERY), TMS, SPC, OTC, FFN, TIL, and CTF. Conclusion: The toxA gene was detected in many Pm isolates from pneumonic lungs. Capsule type A or D was the most frequently found among the collected isolates. LIN, TYL, and ERY are the drugs which showed higher percentages of resistant isolates.

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Protection against progressive atrophic rhinitis by vaccination with Pasteurella multocida toxin purified by monoclonal antibodies

Veterinary Record ◽

10.1136/vr.125.1.7 ◽

1989 ◽

Vol 125 (1) ◽

pp. 7-11 ◽

Cited By ~ 42

Author(s):

N. Foged ◽

J. Nielsen ◽

S. Jorsal

Keyword(s):

Monoclonal Antibodies ◽

Pasteurella Multocida ◽

Atrophic Rhinitis ◽

Pasteurella Multocida Toxin ◽

Progressive Atrophic Rhinitis

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Involvement of Osteocytes in the Action of Pasteurella multocida Toxin

Toxins ◽

10.3390/toxins10080328 ◽

2018 ◽

Vol 10 (8) ◽

pp. 328 ◽

Cited By ~ 1

Author(s):

Hannah Heni ◽

Julia Ebner ◽

Gudula Schmidt ◽

Klaus Aktories ◽

Joachim Orth

Keyword(s):

G Proteins ◽

Pasteurella Multocida ◽

Fiber Formation ◽

Atrophic Rhinitis ◽

Target Cells ◽

Heterotrimeric G Proteins ◽

Culture Model ◽

Tnf Α ◽

Pasteurella Multocida Toxin ◽

Progressive Atrophic Rhinitis

Pasteurella multocida toxin (PMT) causes progressive atrophic rhinitis with severe turbinate bone degradation in pigs. It has been reported that the toxin deamidates and activates heterotrimeric G proteins, resulting in increased differentiation of osteoclasts and blockade of osteoblast differentiation. So far, the action of PMT on osteocytes, which is the most abundant cell type in bone tissue, is not known. In MLO-Y4 osteocytes, PMT deamidated heterotrimeric G proteins, resulting in loss of osteocyte dendritic processes, stress fiber formation, cell spreading and activation of RhoC but not of RhoA. Moreover, the toxin caused processing of membrane-bound receptor activator of NF-κB ligand (RANKL) to release soluble RANKL and enhanced the secretion of osteoclastogenic TNF-α. In a co-culture model of osteocytes and bone marrow cells, PMT-induced osteoclastogenesis was largely increased as compared to the mono-culture model. The enhancement of osteoclastogenesis observed in the co-culture was blocked by sequestering RANKL with osteoprotegerin and by an antibody against TNF-α indicating involvement of release of the osteoclastogenic factors from osteocytes. Data support the crucial role of osteocytes in bone metabolism and osteoclastogenesis and identify osteocytes as important target cells of PMT in progressive atrophic rhinitis.

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