Dramatic improvement in FMD DNA vaccine efficacy and cross-serotype antibody induction in pigs following a protein boost

Vaccine ◽  
2008 ◽  
Vol 26 (21) ◽  
pp. 2647-2656 ◽  
Author(s):  
Yanmin Li ◽  
Catrina M.A. Stirling ◽  
Michael S. Denyer ◽  
Pippa Hamblin ◽  
G. Hutchings ◽  
...  
Keyword(s):  
Dna Vaccine ◽  
Vaccine Efficacy ◽  
Dramatic Improvement ◽  
Protein Boost ◽  
Antibody Induction

scholarly journals Non-Lethal Sequential Individual Monitoring of Viremia in Relation to DNA Vaccination in Fish–Example Using a Salmon Alphavirus DNA Vaccine in Atlantic Salmon Salmo salar

Vaccines ◽  
2021 ◽  
Vol 9 (2) ◽  
pp. 163
Author(s):  
Catherine Collins ◽  
Katherine Lester ◽  
Jorge Del-Pozo ◽  
Bertrand Collet
Keyword(s):  
Dna Vaccine ◽  
Experimental Infection ◽  
Vaccine Efficacy ◽  
Dna Vaccines ◽  
Experimental Studies ◽  
Dna Vaccination ◽  
Line System ◽  
Individual Fish ◽  
Antibody Levels ◽  
Subtype 3

Traditionally, commercial testing for vaccine efficacy has relied on the mass infection of vaccinated and unvaccinated animals and the comparison of mortality prevalence and incidence. For some infection models where disease does not cause mortality this approach to testing vaccine efficacy is not useful. Additionally, in fish experimental studies on vaccine efficacy and immune response the norm is that several individuals are lethally sampled at sequential timepoints, and results are extrapolated to represent the kinetics of immune and disease parameters of an individual fish over the entire experimental infection period. In the present study we developed a new approach to vaccine testing for viremic viruses in fish by following the same individuals over the course of a DNA vaccination and experimental infection through repeated blood collection and analyses. Injectable DNA vaccines are particularly efficient against viral disease in fish. To date, two DNA vaccines have been authorised for use in fish farming, one in Canada against Infectious Haemorrhagic Necrotic virus and more recently one in Europe against Salmon Pancreatic Disease virus (SPDv) subtype 3. In the current study we engineered and used an experimental DNA vaccine against SPDv subtype 1. We measured viremia using a reporter cell line system and demonstrated that the viremia phase was completely extinguished following DNA vaccination. Differences in viremia infection kinetics between fish in the placebo group could be related to subsequent antibody levels in the individual fish, with higher antibody levels at terminal sampling in fish showing earlier viremia peaks. The results indicate that sequential non-lethal sampling can highlight associations between infection traits and immune responses measured at asynchronous timepoints and, can provide biological explanations for variation in data. Similar to results observed for the SPDv subtype 3 DNA vaccine, the SPDv subtype 1 DNA vaccine also induced an interferon type 1 response after vaccination and provided high protection against SPDv under laboratory conditions when fish were challenged at 7 weeks post-vaccination.

The immune response to a DNA vaccine can be modulated by co-delivery of cytokine genes using a DNA prime-protein boost strategy

Vaccine ◽  
2001 ◽  
Vol 19 (28-29) ◽  
pp. 4053-4060 ◽  
Author(s):  
Jean-Pierre Y. Scheerlinck ◽  
Gerard Casey ◽  
Peter McWaters ◽  
Julie Kelly ◽  
David Woollard ◽  
...  
Keyword(s):  
Immune Response ◽  
Dna Vaccine ◽  
Cytokine Genes ◽  
Protein Boost

P3-468: Exploiting prime-boost regimen and various delivery strategies to improve Alzhaimer's disease DNA vaccine efficacy

2010 ◽  
Vol 6 ◽  
pp. S591-S591
Author(s):  
Hayk Davtyan ◽  
Anahit Ghochikyan ◽  
Mikayel Mkrtichyan ◽  
Nina Movsesyan ◽  
Irina Petrushina ◽  
...  
Keyword(s):  
Dna Vaccine ◽  
Vaccine Efficacy ◽  
Boost Regimen ◽  
Delivery Strategies

Synergy of mIL-21 and mIL-15 in enhancing DNA vaccine efficacy against acute and chronic Toxoplasma gondii infection in mice

Vaccine ◽  
2014 ◽  
Vol 32 (25) ◽  
pp. 3058-3065 ◽  
Author(s):  
Zhong-Yuan Li ◽  
Jia Chen ◽  
Eskild Petersen ◽  
Dong-Hui Zhou ◽  
Si-Yang Huang ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Dna Vaccine ◽  
Vaccine Efficacy ◽  
Toxoplasma Gondii Infection

scholarly journals Increase in DNA vaccine efficacy by virosome delivery and co-expression of a cytolytic protein

2014 ◽  
Vol 3 (6) ◽  
pp. e18 ◽  
Author(s):  
Tessa Gargett ◽  
Branka Grubor-Bauk ◽  
Darren Miller ◽  
Tamsin Garrod ◽  
Stanley Yu ◽  
...  
Keyword(s):  
Dna Vaccine ◽  
Vaccine Efficacy

scholarly journals Herpes Simplex Virus DNA Vaccine Efficacy: Effect of Glycoprotein D Plasmid Constructs

2000 ◽  
Vol 182 (5) ◽  
pp. 1304-1310 ◽  
Author(s):  
J. E. Strasser ◽  
R. L. Arnold ◽  
C. Pachuk ◽  
T. J. Higgins ◽  
D. I. Bernstein
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Dna Vaccine ◽  
Vaccine Efficacy ◽  
Glycoprotein D ◽  
Simplex Virus

scholarly journals Effication of Viral Nervous Necrosis DNA as Vaccines for Cromileptes altivelis

2020 ◽  
Vol 3 (2) ◽  
Author(s):  
Wiwien Mukti Andriyani ◽  
Sri Murtini ◽  
Alimuddin Alimuddin
Keyword(s):  
Dna Vaccine ◽  
Challenge Test ◽  
Virus Titer ◽  
Dna Vaccination ◽  
Post Vaccination ◽  
Viral Nervous Necrosis ◽  
Antibody Induction ◽  
Actin Promoter ◽  
Two Stages ◽  
Made In

Viral nervous necrosis (VNN) is a disease that often infects groupers. It has caused mass death in more than 34 species of marine fish. DNA vaccination might become a solution againts the infection. The construction of the pmBA-CP DNA vaccine consisting of the beta-actin promoter of medaka fish (Oryzias latipes) and capsid protein (CP) encoding VNN RNA2 has been made in previous studies. This study aimed to test the efficacy of the pmBA-CP DNA vaccine for VNN. The experiment consisted of two stages, namely (1) detection of anti-VNN antibody induction in vaccinated fish using ELISA, and (2) challenge test for fish vaccinated with the VNN virus. Grouper (body length 8 cm to 10 cm) were divided into two groups with a density of 5 fish 60 L–1. The fish in the first group were vaccinated with pmBA-CP intramuscularly at a dose of 12.5 µg per fish, while the second group of fish were not vaccinated. Antibody titer testing was carried out before treatment, and 1 d, 7 d, 14 d, 21 d, 28 d, and 35 d after vaccination. The challenge test was carried out on the 60th day after vaccination. The results showed that the S / P ratio in the vaccinated fish serum was higher than unvaccinated fish at 21 d to 35 d post-vaccination. DNA vaccination was able to induce anti-VNN antibodies of grouper. The results of the challenge test for vaccinated fish using VNN virus titer 103.5 FID50/0.2 mL showed 60% of survival rate. Thus, the pmBA-CP DNA vaccine could be useful for increasing grouper immunity, and support production of grouper.

Sign in / Sign up

Export Citation Format

Share Document