Effect of different types of feed (squid, sea worms, and trash fish) to gonad maternity and fundamental levels of Galah shrimp (Macrobrachium Rosenbergii)

Giant prawns (Macrobrachium rosenbergii) need enough nutrients for the reproductive process. One way to give feed which contains high enough protein. Complete nutritional content, especially protein, can be found in natural food. The type of natural feed used for main feeds including squid, sea worms, trash fish. Complete nutrient content can affect the rate of gonadal development and fecundity. (Squid, sea worms, trash fish) on the level of gonadal maturity and density of broodstock parents (Macrobrachium rosenbergii). This research was conducted by a completely randomized design (CRD) trial design. This study used 4 replications of different types of feeding. The types of feed given are squid, sea worms, trash fish, and pellets at a dose of 5% from biomass/day. The parameters of observation include observation of the level of gonad resistance, fundamentality of broodstock parent, and measurement of water quality (temperature, pH, and dissolved oxygen). The results showed that the administration of different types of feed (sea worms, trash fish) had a very significant effect on the level of maturity and had a significant effect on the probability of prawns (M. rosenbergii). After analysis of variance, the results of F Calculations are greater than F Table 0.05 and F Table 0.01. The smallest significant difference test (LSD) was obtained in P1 (Squid feed) with the Gonad maturity rate ± 5- 6 days as much as 83 % parent and fecundity average results of 28,846 ± 4,925.3 thousand / 45 g.

Analysis of catching of sea cucumber (Stichopus variegatus) in Kramian islands, Sumenep regency, East Java

Kramian Island is located in the north of the Masalembu Islands, East Java. This area has good sea cucumber resources, but the rules on the prohibition of overfishing of sea cucumbers have not been implemented by the government in the Kramian Islands, so that fishing activities carried out by fishermen, continuously regardless of the type and size of sea cucumbers, can cause sea cucumbers in the wild to run out and the impact of sea cucumbers will be extinct. The research was conducted with the aim of knowing the fishing location, fishing method, sea cucumber species, number of catches, and benefits of sea cucumber catch. This research was conducted in March-June 2020. The method used in the study was observed with fishermen. The results of the discovery of the location of sea cucumbers using GPS, there were 3 types of sea cucumbers, namely Tanduk (Stichopus variegatus), Kapuk (Stichopus variegatus), and Susu (Holothuria rigida). Sea cucumber catch in March (253.4 kg), April (261.1 kg), May (124.1 kg), June (733.6 kg). The highest sea cucumber catch data occurred in June, while the lowest catch occurred in May.

Analysis of Vannamei shrimp DNA fragment resistant to White Spot Virus Syndrome

The attack of WSSV in Vannamei shrimp cultivation is still common. Shrimp quality improvement can be made through selection with the help of markers (marker-assisted choice). This study aimed to evaluate the DNA fragment profile of white shrimp that was resistant to WSSV disease. The analysis was performed using the PCR-RAPD method. WSSV challenged four groups of 100 Vannamei shrimp, then DNA was extracted from live and dead shrimp. The results showed that 2 of the 17 primers tested had high potential as markers, namely OP-09 and OPD-2. PCR products with OPC-09 primers had specific DNA bands measuring about 1.2 kb in all post-challenge WSSV resistant shrimp individuals. The amplification results using OPD-02 primers showed a particular band of DNA with a length of about 1.0 kb, with 60 % of the appearance in WSSV-resistant shrimp. In contrast, the WSSV-susceptible shrimp group did not have specific DNA fragments. Thus, the two RAPD primers had a high chance of being used in the selection with the help of markers to produce WSSV resistant shrimp.

Implication of total replacement of fish oil with vegetable oils on nutritional and lipid profiles of fish

In this study, the effects of using vegetable oils solely in finfish feed on nutritional status as well as lipid damage were evaluated. Polyene index (PI), atherogenic index (AI) as well as hypocholesterolemic and hypercholesterolemic fatty acids were assessed. Also, n-3/n-6 and n-6/n-3 ratios were assessed. In all, 32 articles were used for this study after carefully assessing them. Eighteen (18) articles had studied Freshwater species (FWF) whiles fourteen (14) had studied Marine species (MF). TI, n-3/n-6, and n-6/n-3 were significantly higher in fish-fed saturated fatty acids (SFA), n-3, and n-6 dominant vegetables. We document that using vegetable oils without supplementing fish diets with fish oil does not compromise the nutritional status as well as lipid damage. This work provides important information about the effect of using 100% vegetable oils in fish with respect to the nutritional composition of the final product.

Native and non-native ornamental aquarium fishes of Bangladesh

The study was conducted in Dhaka, Bangladesh. It was carried out for twelve (12) months from March 2018 to February 2019 to prepare a complete update checklist of native and non-native aquarium fishes of Bangladesh. During the current study, 270 varieties (230 freshwater, 36 marine, and 4 brackish water) belong to 149 species (109 freshwater 73 %, 36 marines 24 %, and 4 brackish water 3 %) of 38 families under 10 orders and 6 crossbreeds’ varieties were recorded. Considering the number of species maximum 83 (55.70 %) was found under the order Perciformes followed by Cypriniformes 24 (16.10 %), Characiformes 18 (12.08 %), Siluriformes 11 (7.38 %), Osteoglossiformes 05 (3.35 %), Atheriniformes 03 (2.01 %), Lepisosteiformes 02 (1.34 %), Polypteriformes 01 (0.6 %), Myliobatiformes 01 (0.67 %) and Cyprinodontiformes 01 (0.67 %). The top five popular species were guppy (13.16 %) followed by goldfish (12.39 %), molly (8.54 %), angelfish (6.23 %), platy (5.93 %). The number of fish species' increasing tendency was 5.96 times in the last 15 years, and 3.31 times in the last ten years. Local farms and aquarists breeders bred 76 varieties under 23 species due to its high demand and profitability. Pricing varied on varieties, species, size, and breeding status (local or abroad), availability, and ranged from BDT 40.00-80,000.00 per pair. According to the findings, aquarium fisheries are highly profitable and will be a potential sector in Bangladesh.

Utilization of Papaya Leaves (Carica papaya) in Feed on Growth of Catfish (Clarias sp.) in Muara Enim, South Sumatera

The feed must have a quality that matches the needs of the fish to produce high fish growth. Feed quality can be improved by adding alternative ingredients to feed. One alternative material that can be used is papaya leaves. The purpose of this study was to determine the effect of adding papaya leaves to feed on the growth of catfish (Clarias sp). This research was conducted in Muara Enim, South Sumatra in August 2020. The treatments were P0: control (without adding papaya leaves) and P1: with the addition of papaya leaves. The data observed included absolute growth, specific growth rate, feed efficiency, and survival. Fish maintenance data that obtained in treatment P0, that absolute weight growth of 8.43 g, absolute length growth 5.13 cm, specific growth rate 6.41 %, feed efficiency 95.77 %, and survival 76 %, while in P1 treatment using leaves papaya produced absolute weight growth of 10.57 g, absolute length growth of 5.17 cm, the specific growth rate of 5.84 %, feed efficiency 100.83 %, and survival 60 %. The results obtained indicate that the provision of papaya leaves affects the growth of catfish.

Analysis of fresh water ornamental fish production (Goldfish, Koi and Betta Fish) in Tulungagung district, East Java, Indonesia

Freshwater ornamental fish such as carp, koi, and betta fish is a kind of a very potential happy developed in the Tulungagung district. The total production in the last 3 years is still above the average production of other freshwater ornamental fish. This study aims to know the best influence on the production of freshwater ornamental fish by the influence of capital, cultivation pond, manpower, and technology. The method of research used is by the purposive sampling method of predetermined culture. Furthermore, the data that has been in can be inserted into SPSS 18 which is in multiple linear regression. The results of this research show that on the cultivation of goldfish, the production factor has a significant effect on the production and the technology variable has the highest beta coefficient of 0.364. For the cultivation of Koi fish, the production factor does not have a significant influence on the production, and the technology variable has the highest beta coefficient of 0.462. For betta fish farming, production factors have no significant effect on production variable and capital variables have the highest beta coefficient of 0.353 which means that production variables are heavily influenced by modal variables. Based on the results of the research can be concluded that out of all production factors, technology has the most influential likelihood on the marketing of freshwater ornamental fish.

Optimization of Anguilla bicolor oxygen consumption in alkalinity culture media

One potential aquaculture commodity that can be developed is Anguilla bicolor. One effort that can be conducted to maximize the life and growth of A. bicolor by manipulating water quality through alkalinity parameters. Alkalinity is a buffer capacity for changing in water pH, and osmotic pressure of water. Alkalinity levelss in culture media can be managed by the addition of calcium carbonate (CaCO3), calcium oxide (CaO), or calcium hydroxide (CaOH) with a certain dose to the culture media. This study aimed to determine the effect of alkalinity on aquaculture media based on the level of oxygen consumption (OC) that related to growth value. This research was conducted with an experimental design using a completely randomized design (CRD) with four treatments, namely the addition of CaCO3 to maintenance media with different doses (A, 0 mg/L; B, 50 mg/L; C, 100 mg/L; D, 150 mg/L). The results showed that the treatment B (50 mg / L) showed an OC value (0.15 mgO2/g/h) and an optimal Growth Rate (GR) (3.75 g/day), while the Survival Rate (SR) parameter, the treatment had no significant effect. The conclusion was 50 mg/L of calcium carbonate (CaCO3) having the optimum alkalinity level in A. bicolor culture media. At the additional dose of CaCO3, the growth value (GR), and the level of eel fish oxygen consumption were optimal.

Effication of Viral Nervous Necrosis DNA as Vaccines for Cromileptes altivelis

Viral nervous necrosis (VNN) is a disease that often infects groupers. It has caused mass death in more than 34 species of marine fish. DNA vaccination might become a solution againts the infection. The construction of the pmBA-CP DNA vaccine consisting of the beta-actin promoter of medaka fish (Oryzias latipes) and capsid protein (CP) encoding VNN RNA2 has been made in previous studies. This study aimed to test the efficacy of the pmBA-CP DNA vaccine for VNN. The experiment consisted of two stages, namely (1) detection of anti-VNN antibody induction in vaccinated fish using ELISA, and (2) challenge test for fish vaccinated with the VNN virus. Grouper (body length 8 cm to 10 cm) were divided into two groups with a density of 5 fish 60 L–1. The fish in the first group were vaccinated with pmBA-CP intramuscularly at a dose of 12.5 µg per fish, while the second group of fish were not vaccinated. Antibody titer testing was carried out before treatment, and 1 d, 7 d, 14 d, 21 d, 28 d, and 35 d after vaccination. The challenge test was carried out on the 60th day after vaccination. The results showed that the S / P ratio in the vaccinated fish serum was higher than unvaccinated fish at 21 d to 35 d post-vaccination. DNA vaccination was able to induce anti-VNN antibodies of grouper. The results of the challenge test for vaccinated fish using VNN virus titer 103.5 FID50/0.2 mL showed 60% of survival rate. Thus, the pmBA-CP DNA vaccine could be useful for increasing grouper immunity, and support production of grouper.

The relationship of redox potential to crustacea abundance at Bama beach of Baluran National Park, Situbondo, Indonesia

Catfish farming is growing rapidly because the cultivation and marketing technology is relatively easy for the community to master, and the required business capital is relatively small. Pond water waste originating from fish metabolism and dissolved food waste is toxic to fish. One of the efforts that can be done is fish farming that is integrated with plants through an aquaponics system. Research to determine the effect of AB Mix addition on the growth of African catfish and lettuce cultivated by the aquaponics system. The research method used was an experimental method with a completely randomized design (CRD) consisting of three treatments and three replications. The addition of AB mixed nutrition did not differ in the growing significance of catfish and lettuce. However, the increase in growth occurred with an increasing dosage of AB mix with the best treatment with AB Mix 3 mL L-1. This treatment resulted in absolute growth in fish of 20.52 g and 4.62 g of watercress. The use of a dosage of AB mix higher than 3 mL L-1 may result in better growth.