Strong HCV NS3- and NS4A-specific cellular immune responses induced in mice and Rhesus macaques by a novel HCV genotype 1a/1b consensus DNA vaccine

To improve the lower immune intensity of DNA vaccines, we developed a DNA vaccine based on prostate cancer-specific antigen (PSA), which has been suggested as a potential target for prostate cancer therapy, and enhanced the DNA vaccine potency using interleukin-12 (IL-12) as an intramolecular adjuvant. A series of DNA plasmids encoding human PSA, IL-12, and their conjugates was constructed and injected into female mice intramuscularly, followed by an electric pulse. The humoral and cellular immune responses after immunization were detected by ELISA and ELISPOT, respectively. To evaluate the therapeutic efficacy of these plasmids, a mouse model with a PSA-expressing tumor was constructed. Mice vaccinated with PSA-IL-12 plasmids elicited the strongest PSA-specific humoral and cellular immune responses. Furthermore, these vaccinations inhibited the growth of PSA-expressing tumors and prolonged mouse survival. These observations emphasize the potential of the IL-12 gene as an intramolecular adjuvant for DNA vaccines. Moreover, the vaccine based on PSA and IL-12 may be a promising treatment for prostate cancer.

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Evaluation of humoral and cellular immune responses against HSV-1 using genetic immunization by filamentous phage particles: A comparative approach to conventional DNA vaccine

Journal of Virological Methods ◽

10.1016/j.jviromet.2009.11.008 ◽

2010 ◽

Vol 163 (2) ◽

pp. 440-444 ◽

Cited By ~ 21

Author(s):

Hamidreza Hashemi ◽

Taravat Bamdad ◽

Abbas Jamali ◽

Somayeh Pouyanfard ◽

Masoumeh Gorgian Mohammadi

Keyword(s):

Immune Responses ◽

Dna Vaccine ◽

Comparative Approach ◽

Filamentous Phage ◽

Genetic Immunization ◽

Cellular Immune Responses ◽

Cellular Immune ◽

Hsv 1

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The Humoral and Cellular Immune Responses in Mice Induced by DNA Vaccine Expressing the Sporozoite Surface Protein ofCryptosporidium parvum

DNA and Cell Biology ◽

10.1089/104454904323090967 ◽

2004 ◽

Vol 23 (5) ◽

pp. 335-339 ◽

Cited By ~ 17

Author(s):

Hongxuan He ◽

Baohua Zhao ◽

Liyan Liu ◽

Kai Zhou ◽

Ximing Qin ◽

...

Keyword(s):

Immune Responses ◽

Dna Vaccine ◽

Surface Protein ◽

Cellular Immune Responses ◽

Cellular Immune

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Th-1-Type Cytotoxic CD8+ T-Lymphocyte Responses to Simian Immunodeficiency Virus (SIV) Are a Consistent Feature of Natural SIV Infection in Sooty Mangabeys

Journal of Virology ◽

10.1128/jvi.80.6.2771-2783.2006 ◽

2006 ◽

Vol 80 (6) ◽

pp. 2771-2783 ◽

Cited By ~ 50

Author(s):

Zichun Wang ◽

Benjamin Metcalf ◽

Ruy M. Ribeiro ◽

Harold McClure ◽

Amitinder Kaur

Keyword(s):

T Lymphocytes ◽

Immune Responses ◽

Rhesus Macaques ◽

Elispot Response ◽

Cellular Immune Responses ◽

Immunodeficiency Virus ◽

Sooty Mangabeys ◽

Siv Infection ◽

Ifn Γ ◽

Cellular Immune

ABSTRACT Sooty mangabeys are a natural host of simian immunodeficiency virus (SIV) that remain asymptomatic and do not exhibit increased immune activation or increased T-lymphocyte turnover despite sustained high levels of SIV viremia. In this study we asked whether an altered immune response to SIV contributes to the lack of immunopathology in sooty mangabeys as opposed to species with pathogenic lentivirus infection. SIV-specific cellular immune responses were investigated in a cohort of 25 sooty mangabeys with natural SIV infection. Gamma interferon (IFN-γ) enzyme-linked immunospot (ELISPOT) assay responses targeting a median of four SIV proteins were detected in all 25 mangabeys and were comparable in magnitude to those of 13 rhesus macaques infected with SIVmac251 for more than 6 months. As with rhesus macaques, Th2 ELISPOT responses to SIV were absent or >10-fold lower than the IFN-γ ELISPOT response to the same SIV protein. The SIV-specific ELISPOT response was predominantly mediated by CD8+ T lymphocytes; the frequency of circulating SIV-specific CD8+ T lymphocytes ranged between 0.11% and 3.26% in 13 mangabeys. Functionally, the SIV-specific CD8+ T lymphocytes were cytotoxic; secreted IFN-γ, tumor necrosis factor alpha, and macrophage inflammatory protein 1β; and had an activated effector phenotype. Although there was a trend toward higher frequencies of SIV-specific CD8+ T lymphocytes in mangabeys with lower viral loads, a significant inverse correlation between SIV viremia and SIV-specific cellular immunity was not detected. The consistent detection of Th1-type SIV-specific cellular immune responses in naturally infected sooty mangabeys suggests that immune attenuation is neither a feature of nor a requirement for maintenance of nonpathogenic SIV infection in its natural host.

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