scholarly journals Characterization and comparison of strains of Pasteurella multocida associated with cases of progressive atrophic rhinitis and porcine pneumonia in Argentina

2019 ◽  
Vol 12 (3) ◽  
pp. 434-439
Author(s):  
Fernando A. Bessone ◽  
Maria Laura Soriano Perez ◽  
Gustavo Zielinski ◽  
Marina Dibarbora ◽  
M. B. Conde ◽  
...  
Keyword(s):  
Antibiotic Susceptibility ◽  
Pasteurella Multocida ◽  
Type A ◽  
Economic Loss ◽  
Diffusion Method ◽  
Atrophic Rhinitis ◽  
Specific Gene ◽  
Capsular Type ◽  
Progressive Atrophic Rhinitis ◽  
Susceptibility Profiles

Background: Pasteurella multocida (Pm) is the causative agent of progressive atrophic rhinitis (PAR) and pneumonic pasteurellosis (PN) in pigs. Pm is a member of the porcine respiratory complex responsible for important economic loss in the pig industry. Aim: This study aimed to characterize the Pm strains recovered from clinical cases of PN and PAR and to elucidate the antibiotic susceptibility profiles of the strains. Materials and Methods: Sixty strains were characterized molecularly by polymerase chain reaction to determine species-specific gene, capsular type (A or D), and toxin A production. The agar diffusion method was employed to evaluate antibiotic resistance profiles. Results: We found that 65% of strains belonged to capsular type A or D, and 15% of those were positive to toxA gene. The antibiotic susceptibility profiles found were sensitive in decreasing order to: Enrofloxacin, ceftiofur (CTF), ampicillin, tilmicosin (TIL), florfenicol (FFN), spectinomycin (SPC), gentamicin, oxytetracycline (OTC), and trimethoprim-sulfamethoxazole (TMS). Strains were resistant in decreasing order to: Lincomycin (LIN), tylosin (TYL), erythromycin (ERY), TMS, SPC, OTC, FFN, TIL, and CTF. Conclusion: The toxA gene was detected in many Pm isolates from pneumonic lungs. Capsule type A or D was the most frequently found among the collected isolates. LIN, TYL, and ERY are the drugs which showed higher percentages of resistant isolates.

scholarly journals Molecular Fingerprinting of Pasteurella Multocida Associated with Progressive Atrophic Rhinitis in Swine Herds

1994 ◽  
Vol 6 (4) ◽  
pp. 442-447 ◽  
Author(s):  
Ian A. Gardner ◽  
Rick Kasten ◽  
Graeme J. Eamens ◽  
Kurt P. Snipes ◽  
Randall J. Anderson
Keyword(s):  
Pasteurella Multocida ◽  
Uv Light ◽  
Type A ◽  
Atrophic Rhinitis ◽  
Capsular Type ◽  
Molecular Fingerprinting ◽  
Whole Cell ◽  
Type D ◽  
Progressive Atrophic Rhinitis ◽  
Swine Herds

Ninety-six nasal isolates of Pasteurella multocida from swine herds with progressive atrophic rhinitis were characterized by restriction endonuclease analysis (REA) of whole-cell DNA, ribotyping, and plasmid analysis. For REA, bacterial DNA was digested with SmaI and electrophoresed in 0.7% agarose, and fragments were visualized with UV light. For ribotyping, EcoRI-digested and electrophoresed restriction fragments of whole-cell DNA were transferred to nitrocellulose membranes, hybridized with γ-32P-labeled Escherichia coli ribosomal RNA, and visualized by autoradiography. Phenotypes of isolates were toxigenic capsular type D ( n = 51), nontoxigenic type D ( n = 28), nontoxigenic type A ( n = 16), and toxigenic type A ( n = 1). Plasmids of various sizes were evident in 92.2% and 17.9% of toxigenic and nontoxigenic D strains, respectively, but were absent from all type A strains. Among the 4 phenotypes, there were 17 REA profiles and 6 ribotypes. For 3 of 17 REA patterns, multiple ribotypes were evident, and several REA types were evident in 5 of 6 ribotypes. Thirty-seven isolates of toxigenic capsular type D from Australian herds were either SmaI type B or C and ribotype 2, whereas 14 toxigenic D isolates from the USA and other countries were more heterogeneous (7 REA types and 6 ribotypes). The fingerprinting results provided evidence in support of the hypothesis of a single source infection in Australia associated with the introduction of breeding pigs from overseas.

scholarly journals Protective efficacy of calcium phosphate nanoparticle adsorbed bivalent subunit vaccine of Pasteurella multocida against homologous challenge in mice

2020 ◽  
Author(s):  
Songyukta Shyam ◽  
Shantanu Tamuly ◽  
Probodh Borah ◽  
Rajeev Kumar Sharma
Keyword(s):  
Calcium Phosphate ◽  
Subunit Vaccine ◽  
Pasteurella Multocida ◽  
Protective Efficacy ◽  
Economic Loss ◽  
Capsular Type ◽  
Significant Protection ◽  
Type B ◽  
Serotype A ◽  
Calcium Phosphate Nanoparticle

ABSTRACTSwine pasteurellosis, caused by Pasteurella multocida capsular types A and D, causes heavy economic loss to the pig farmers. The vaccine presently used is abacterin of Pasteurella multocida capsular type B that is proven to be effective against bovine pasteurellosis. However, its efficacy against swine pasteurellosis is questionable. The present study was carried out to evaluate the efficacy of calcium phosphate nanoparticle adjuvanted bivalent subunit vaccine prepared from Pasteurella multocida capsular types A and D along with a monovalent subunit vaccine prepared from Pasteurella multocida capsular type B in mice. The Alum precipitated bacterin vaccine was used as the control. The bivalent subunit vaccine comprising the immune components of both the capsular types showed significantly higher IgG response than either of the other two vaccines. Both the calcium phosphate nanoparticle adjuvanted vaccines could elicit 100% protection in mice against homologous challenges but the aluminum hydroxide adjuvanted bacterin vaccine could not elicit significant protection. Based on this preliminary work, it was concluded that the bivalent subunit vaccine would be a better option for immunization of swine against swine pasteurellosis.IMPORTANCE OF THE WORKThe swine pasteurellosis is an important economic disease affecting the pig population in the North-eastern part of India that contributes the major pig population. The disease is caused by Serotype A and D of Pasteurella multocida. At present the inactivated vaccine is used that is actually developed against P52 strain of serotype B:2 of Pasteurella multocida, which is mainly involved in haemorrhagic septicaemia (or bovine pasteurellosis) that affects the cattle, buffaloe, sheep and goat. As a result, the present vaccine does not give sufficient protection in pigs but gives significant protection in cattle, buffaloe, sheep and goat. Hence, there is a need of development of vaccine that can address specifically swine pasteurellosis by targeting serotype A and D of Pasteurella multocida.

Efficacy of a novel Pasteurella multocida vaccine against progressive atrophic rhinitis of swine

Vaccine ◽  
2009 ◽  
Vol 27 (22) ◽  
pp. 2923-2929 ◽  
Author(s):  
Shih-Ling Hsuan ◽  
Chih-Ming Liao ◽  
Chienjin Huang ◽  
James R. Winton ◽  
Zeng-Weng Chen ◽  
...  
Keyword(s):  
Pasteurella Multocida ◽  
Atrophic Rhinitis ◽  
Progressive Atrophic Rhinitis

scholarly journals Characterization of culturable airborne bacteria and antibiotic susceptibility profiles of indoor and immediate-outdoor environments of a research institute

2018 ◽  
Vol 1 ◽  
pp. 17
Author(s):  
Isawumi Abiola ◽  
Adiza Abass ◽  
Samuel Duodu ◽  
Lydia Mosi
Keyword(s):  
Antibiotic Susceptibility ◽  
Diffusion Method ◽  
Antibiotics Resistance ◽  
Contamination Rate ◽  
Beta Lactam ◽  
Disk Diffusion Method ◽  
Outdoor Environments ◽  
Public Health Challenges ◽  
Susceptibility Profiles ◽  
Research Institute

Background:The study was conducted to determine the bacterial composition and antibiotic susceptibility profiles of a research institute at the University of Ghana where workers and students spend about 70-85% of their lives in indoor and immediate-outdoor environments. This is imperative as one-third of the recognized infectious diseases are transmitted through airborne-route. Furthermore, the increasing rate of bacterial antimicrobial resistance associated with such environments poses serious public health challenges.Methods:A total of 42 airborne samples were collected from eight major sites at the Department of Biochemistry, Cell and Molecular Biology (BCMB), using passive bacterial sampling techniques. Standard phenotypic microbiological procedures were used to characterize the isolates. Antibiotic susceptibility profiles were determined using standard disk diffusion method and guidelines of Clinical and Laboratory Standards Institute (CLSI).Results:Four groups of bacterial isolates were identified from the total samples collected with Gram positive bacilli as the most common. All the isolates showed resistance to beta lactam and sulfonamide classes of antibiotics with full resistance (100%) to ampicillin and penicillin. In total, seven different anti-biotypes were observed with the highest susceptibility displayed towards tetracycline and gentamycin. Significantly, the various air sampling sites of the institute indicated the presence of bacteria with the majority showing multiple antibiotics resistance.Conclusions:Although the recovery of bacteria from supposed sterile environments calls for attention, the observed low contamination rate as compared to the WHO standard suggests a minimum risk of exposure of students and workers to airborne microbial contamination.

scholarly journals Antibiotic susceptibility pattern of isolates of Pseudomonas aeruginosa in a Saudi Arabian Hospital

2013 ◽  
Vol 13 (1) ◽  
pp. 45-48
Author(s):  
Shamweel Ahmed ◽  
Mohmammed Nawaf Al-Harbi
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antimicrobial Susceptibility ◽  
Antibiotic Susceptibility ◽  
Clinical Laboratory ◽  
Medical Science ◽  
State Level ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Resistance Patterns ◽  
Susceptibility Profiles

Pseudomonas aeruginosa is one of the most common gram-negative bacteria. identified in the clinical specimens of hospital admitted patients. A major problem in P. aeruginosa infection may be that this pathogen exhibits a high degree of resistance to a broad spectrum of antibiotics. The study aimed to isolate and determine the antimicrobial susceptibility patterns of the P. aeruginosa. This prospective study was done over a period of six months. Forty one clinical isolates of Pseudomonas aeruginosa (P. aeruginosa) were isolated from sputum specimens of the patients suspected of having respiratory tract infection. The antibiotic susceptibility profiles of all the isolates were determined using disk diffusion method as recommended by Clinical Laboratory Standards Institute. Ciprofloxacin was found to be the most effective antimicrobial agent with 85.4% susceptibility followed by imipenem (75.6%), aminoglycosides (amikacin, 95.1% and gentamicin, 90.3%), and the beta-lactams (cefepime 65.8%, ceftazidime, 51.2%). Piperacillin showed the maximum resistance (46.3%) followed by Aztreonam (36.6%). Regular antimicrobial susceptibility surveillance is essential for area-wise monitoring of the resistance patterns. An effective national and state level antibiotic policy and draft guidelines should be introduced to preserve the effectiveness of antibiotics and for better patient management. DOI: http://dx.doi.org/10.3329/bjms.v13i1.17428 Bangladesh Journal of Medical Science Vol. 13 No. 01 January2014: 45-48. Table I & Figure I added on 02 February 2014.

Complete Genome Sequence of a Novel T7-Like Bacteriophage from a Pasteurella multocida Capsular Type A Isolate

2018 ◽  
Vol 75 (5) ◽  
pp. 574-579 ◽  
Author(s):  
Yibao Chen ◽  
Erchao Sun ◽  
Jiaoyang Song ◽  
Lan Yang ◽  
Bin Wu
Keyword(s):  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Pasteurella Multocida ◽  
Type A ◽  
Capsular Type
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