A non-radioactive method for angiotensin II receptor binding studies using the rat liver

2006 ◽  
Vol 53 (3) ◽  
pp. 206-214 ◽  
Author(s):  
Maziar Mohammad Akhavan ◽  
Soltan Ahmed Ebrahimi ◽  
Massoud Mahmoudian
Keyword(s):  
Angiotensin Ii ◽  
Rat Liver ◽  
Receptor Binding ◽  
Angiotensin Ii Receptor ◽  
Binding Studies

Effect of age on angiotensin II receptor binding in rat liver

1995 ◽  
Vol 18 (3) ◽  
pp. 215-216 ◽  
Author(s):  
Sunghou Lee ◽  
Yi-Sook Jung ◽  
Jaeyang Kong
Keyword(s):  
Angiotensin Ii ◽  
Rat Liver ◽  
Receptor Binding ◽  
Angiotensin Ii Receptor ◽  
Effect Of Age

Receptor binding radiotracers for the angiotensin II receptor: Radioiodinated [Sar13,Ile8] angiotensin II

1994 ◽  
Vol 21 (4) ◽  
pp. 593-600 ◽  
Author(s):  
Raymond E. Gibson ◽  
Holly T. Beauchamp ◽  
Christine Fioravanti ◽  
Nancy Brenner ◽  
H.Donald Burns
Keyword(s):  
Angiotensin Ii ◽  
Receptor Binding ◽  
Angiotensin Ii Receptor

scholarly journals Deglycosylation and fragmentation of purified rat liver angiotensin II receptor: application to the mapping of hormone-binding domains

1993 ◽  
Vol 289 (1) ◽  
pp. 289-297 ◽  
Author(s):  
F Desarnaud ◽  
J Marie ◽  
C Lombard ◽  
R Larguier ◽  
R Seyer ◽  
...  
Keyword(s):  
Angiotensin Ii ◽  
Rat Liver ◽  
Transmembrane Domain ◽  
Selective Adsorption ◽  
Structural Data ◽  
Hormone Binding ◽  
Angiotensin Ii Receptor ◽  
Receptor Complexes ◽  
Binding Domains ◽  
Treatment Conditions

We report new structural data about the rat liver angiotensin II receptor, which belongs to the AT1 subclass. This receptor has been purified at analytical or semi-preparative levels by a previously described strategy involving its photolabelling with a biotinylated azido probe and selective adsorption of the covalent probe-receptor complexes to immobilized streptavidin [Marie, Seyer, Lombard, Desarnaud, Aumelas, Jard and Bonnafous (1990) Biochemistry 29, 8943-8950]. Chemical or enzymic deglycosylation of the purified receptor has shown a shift in its molecular mass from 65 kDa to 40 kDa. Fragmentation of the purified receptor was carried out with V8 protease from Staphylococcus aureus, CNBr and trypsin. It was possible to find trypsin-treatment conditions which allowed production of a 6 kDa probe-fragment complex with a satisfactory yield. Attempts to localize this small fragment (5 kDa after subtraction of the probe contribution) in the recently published rat AT1 receptor sequence are reported. As expected, this fragment is not glycosylated; moreover, its further fragmentation by CNBr induces a very slight decrease in its size. These data support the hypothesis that a receptor sequence comprising the third transmembrane domain and adjacent portions of extra- and intracellular loops is involved in photolabelling by the C-terminal azidophenylalanine of the angiotensin-derived probe. These preliminary results are discussed in terms of future prospects for the characterization of hormone-binding domains of angiotensin II receptors.

Inhibition of angiotensin II receptor binding by quinolone alkaloids fromEvodia rutaecarpa

1998 ◽  
Vol 12 (3) ◽  
pp. 212-214 ◽  
Author(s):  
Hyun Sun Lee ◽  
Won Keun Oh ◽  
Hee Cheol Choi ◽  
Jun Won Lee ◽  
Dae Ook Kang ◽  
...  
Keyword(s):  
Angiotensin Ii ◽  
Receptor Binding ◽  
Angiotensin Ii Receptor

Comparative in vivo effects of irbesartan and losartan on angiotensin II receptor binding in the rat kidney following oral administration

2000 ◽  
Vol 99 (4) ◽  
pp. 331 ◽  
Author(s):  
Maurice E. FABIANI ◽  
Diem T. DINH ◽  
Labrini NASSIS ◽  
David J. CASLEY ◽  
Colin I. JOHNSTON
Keyword(s):  
Angiotensin Ii ◽  
Oral Administration ◽  
Receptor Binding ◽  
Rat Kidney ◽  
Angiotensin Ii Receptor ◽  
In Vivo Effects
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