Phylogenetic analysis of Portuguese Feline Immunodeficiency Virus sequences reveals high genetic diversity

Feline immunodeficiency virus (FIV), a lentivirus, is an important pathogen of domestic cats around the world and has many similarities to human immunodeficiency virus (HIV). A characteristic of these lentiviruses is their extensive genetic diversity, which has been an obstacle in the development of successful vaccines. Of the FIV genes, the envelope gene is the most variable and sequence differences in a portion of this gene have been used to define 5 FIV subtypes (A, B, C, D and E). In this study, the proviral DNA sequence of the V3-V5 region of the envelope gene was determined in blood samples from 31 FIV positive cats from 4 different regions of South Africa. Phylogenetic analysis demonstrated the presence of both subtypes A and C, with subtype A predominating. These findings contribute to the understanding of the genetic diversity of FIV.

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Phylogenetic analysis of Vietnamese isolates of feline immunodeficiency virus: genetic diversity of subtype C

Archives of Virology ◽

10.1007/s00705-002-0954-8 ◽

2003 ◽

Vol 148 (4) ◽

pp. 783-791 ◽

Cited By ~ 19

Author(s):

K. Nakamura ◽

Y. Suzuki ◽

K. Ikeo ◽

Y. Ikeda ◽

E. Sato ◽

...

Keyword(s):

Genetic Diversity ◽

Phylogenetic Analysis ◽

Feline Immunodeficiency Virus ◽

Subtype C ◽

Immunodeficiency Virus

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Subtype Characterization and Zoonotic Potential of Cryptosporidium felis in Cats in Guangdong and Shanghai, China

Pathogens ◽

10.3390/pathogens10020089 ◽

2021 ◽

Vol 10 (2) ◽

pp. 89

Author(s):

Jiayu Li ◽

Fuxian Yang ◽

Ruobing Liang ◽

Sheng Guo ◽

Yaqiong Guo ◽

...

Keyword(s):

Genetic Diversity ◽

Phylogenetic Analysis ◽

Genetic Characterization ◽

Zoonotic Potential ◽

Common Occurrence ◽

High Genetic Diversity ◽

Gp60 Gene ◽

The Common ◽

Subtyping Tool

Cryptosporidiumfelis is an important cause of feline and human cryptosporidiosis. However, the transmission of this pathogen between humans and cats remains controversial, partially due to a lack of genetic characterization of isolates from cats. The present study was conducted to examine the genetic diversity of C. felis in cats in China and to assess their potential zoonotic transmission. A newly developed subtyping tool based on a sequence analysis of the 60-kDa glycoprotein (gp60) gene was employed to identify the subtypes of 30 cat-derived C. felis isolates from Guangdong and Shanghai. Altogether, 20 C. felis isolates were successfully subtyped. The results of the sequence alignment showed a high genetic diversity, with 13 novel subtypes and 2 known subtypes of the XIXa subtype family being identified. The known subtypes were previously detected in humans, while some of the subtypes formed well-supported subclusters with human-derived subtypes from other countries in a phylogenetic analysis of the gp60 sequences. The results of this study confirmed the high genetic diversity of the XIXa subtype family of C. felis. The common occurrence of this subtype family in both humans and cats suggests that there could be cross-species transmission of C. felis.

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Phylogenetic analysis of feline immunodeficiency virus strains from naturally infected cats in Belgium and The Netherlands

Virus Research ◽

10.1016/j.virusres.2014.10.023 ◽

2015 ◽

Vol 196 ◽

pp. 30-36 ◽

Cited By ~ 2

Author(s):

Inge D.M. Roukaerts ◽

Sebastiaan Theuns ◽

Elien R.L. Taffin ◽

Sylvie Daminet ◽

Hans J. Nauwynck

Keyword(s):

Phylogenetic Analysis ◽

The Netherlands ◽

Feline Immunodeficiency Virus ◽

Immunodeficiency Virus ◽

Virus Strains

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Genetic diversity of Brazilian isolates of feline immunodeficiency virus

Archives of Virology ◽

10.1007/s00705-009-0587-2 ◽

2010 ◽

Vol 155 (3) ◽

pp. 379-384 ◽

Cited By ~ 9

Author(s):

Bruno Marques Teixeira ◽

N. Logan ◽

J. C. M. Cruz ◽

J. K. P. Reis ◽

P. E. Brandão ◽

...

Keyword(s):

Genetic Diversity ◽

Feline Immunodeficiency Virus ◽

Immunodeficiency Virus

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The prevalence and genetic diversity of feline immunodeficiency virus and feline leukemia virus among stray cats in Harbin, China

TURKISH JOURNAL OF ZOOLOGY ◽

10.3906/zoo-1706-3 ◽

2018 ◽

Vol 42 (2) ◽

pp. 245-251 ◽

Cited By ~ 1

Author(s):

MEI-QIAO PAN ◽

JIU-CHENG WANG ◽

YA-JUN WANG

Keyword(s):

Genetic Diversity ◽

Feline Immunodeficiency Virus ◽

Leukemia Virus ◽

Feline Leukemia Virus ◽

Stray Cats ◽

Immunodeficiency Virus

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Genetic diversity ofFrankiastrains in root nodules fromHippophae¨ rhamnoidesL.

Botany ◽

10.1139/b07-133 ◽

2008 ◽

Vol 86 (3) ◽

pp. 240-247 ◽

Cited By ~ 1

Author(s):

Li-hong Chen ◽

Jian Liu ◽

Gui-min Yao ◽

Wei Yan

Keyword(s):

Genetic Diversity ◽

Phylogenetic Analysis ◽

Restriction Analysis ◽

Root Nodules ◽

Plant Cover ◽

Practical Importance ◽

Intergenic Spacer ◽

Rflp Analysis ◽

Northern China ◽

High Genetic Diversity

The Hippophae¨ rhamnoides L. – Frankia symbiosis is of ecological and practical importance, but very little is known about H. rhamnoides-infective Frankia strains. To address this problem, we have used PCR-restriction fragment length polymorphism (PCR-RFLP) analysis of nifD–nifK intergenic spacer (IGS) to estimate their genetic diversity at 19 sites in Northern China. Restriction analysis indicated that H. rhamnoides-infective Frankia had a high genetic diversity; the samples were divided into nine RFLP patterns (A–I). Elevation and precipitation likely affect the distribution of different Frankia patterns in root nodules. The patterns A and D were present in relatively large areas, which were located at various elevations; however, the distribution of patterns B, C, E, F, G, H, and I generally followed a geographic range. The richness of Frankia diversity was influenced by plant cover and geographic factors such as elevation and precipitation. H. rhamnoides cover had a higher diversity than that of natural vegetation cover. The center part of the geographical range, with intermediate elevation and precipitation, had a higher level of Frankia diversity than that of the west part and east part with high or low elevations and precipitations, respectively. The nifD–nifK IGS regions were sequenced from 28 nodule samples. Phylogenetic analysis showed that H. rhamnoides-infective Frankia strains were all clustered with the Elaeagnus group, and the diversity of this group was quite extensive. Phylogenetic relationships between Hippophae¨ and Elaeagnus-infective Frankia strains were relatively close to each other. Although not very close to either Hippophae¨- or Elaeagnus-infective Frankia strains, Shepherdia -infective strain SCN10a was closer to Hippophae¨-infective strains than to Elaeagnus- infective strains. This is the first detailed report on the genetic diversity and phylogenetic analysis of H. rhamnoides-infective Frankia.

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High genetic diversity of hepatitis delta virus in eastern Turkey

The Journal of Infection in Developing Countries ◽

10.3855/jidc.3910 ◽

2014 ◽

Vol 8 (01) ◽

pp. 074-078 ◽

Cited By ~ 4

Author(s):

Yasemin Bulut ◽

Ibrahim Halil Bahcecioglu ◽

Cem Aygun ◽

Pinar Demirel Oner ◽

Ibrahim Ozercan ◽

...

Keyword(s):

Genetic Diversity ◽

Phylogenetic Analysis ◽

Restriction Enzyme ◽

Hepatitis Delta Virus ◽

Rt Pcr ◽

Genotype I ◽

High Genetic Diversity ◽

Hepatitis Delta ◽

Hdv Genotype ◽

Delta Virus

Introduction: Hepatitis delta virus (HDV) is a serious cause of liver-related mortality in patients infected with hepatitis B virus (HBV). Determination of genotypes of HDV and phylogenetic analysis are important for better understanding the pathogenesis of the liver diseases associated with HBV infection. The aim of this study was to determine the genotype or genotypes of HDV among chronically infected patients with HBV in eastern Turkey. Methodology: A group of 113 patients infected with HBV and HDV were included in this study. The samples taken from the patients were analyzed by reverse transcriptase-polymerase chain reaction (RT-PCR) and restriction enzyme cleavage. Results: According to the results of the restriction enzyme analysis, all of the RT-PCR products were determined to be HDV genotype I. Furthermore, for phylogenetic analysis and genotyping, 40 of HDV RT-PCR positive products were sequenced. Phylogenetic analysis of the sequences showed that all of the samples were infected with HDV genotype I. In addition, the results of the alignment analysis showed that the sequences of clinical samples were 82%-95% similar. Conclusion: These results indicate that high genetic diversity of the virus is possible in endemic areas such as Turkey.

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