First detection of Schmallenberg virus RNA in bovine semen, Germany, 2012

2013 ◽  
Vol 167 (3-4) ◽  
pp. 289-295 ◽  
Author(s):  
Bernd Hoffmann ◽  
Claudia Schulz ◽  
Martin Beer
Keyword(s):  
Schmallenberg Virus ◽  
Bovine Semen ◽  
Virus Rna

scholarly journals Detection of Schmallenberg Virus RNA in Bull Semen in Poland

2016 ◽  
Vol 19 (3) ◽  
pp. 655-657 ◽  
Author(s):  
J. Kęsik-Maliszewska ◽  
M. Larska
Keyword(s):  
Extraction Method ◽  
Rna Extraction ◽  
Viral Rna ◽  
Schmallenberg Virus ◽  
Rt Pcr ◽  
Bovine Semen ◽  
Bull Semen ◽  
Virus Rna ◽  
Virus Excretion ◽  
Venereal Transmission

Abstract The detection of Schmallenberg virus (SBV) in the breeding bull semen raised the question of the possibility of venereal transmission of SBV which could result in cost-intensive restrictions in the trade of bovine semen. In order to evaluate the presence of SBV RNA in bovine semen, 131 bull semen samples from four locations in Poland collected between 2013 and 2015 were analysed by RT-PCR for viral RNA. SBV RNA was detected in 5.3% of the samples. The study has revealed that application of an appropriate RNA extraction method is crucial to detect virus excretion via semen.

Examining bull semen for residues of Schmallenberg virus RNA

2021 ◽  
Author(s):  
Akbar Dastjerdi ◽  
S. Anna La Rocca ◽  
Siva Karuna ◽  
Christopher Finnegan ◽  
Julie Peake ◽  
...  
Keyword(s):  
Schmallenberg Virus ◽  
Bull Semen ◽  
Virus Rna

Organ distribution of Schmallenberg virus RNA in malformed newborns

2012 ◽  
Vol 159 (1-2) ◽  
pp. 236-238 ◽  
Author(s):  
S. Bilk ◽  
C. Schulze ◽  
M. Fischer ◽  
M. Beer ◽  
A. Hlinak ◽  
...  
Keyword(s):  
Schmallenberg Virus ◽  
Organ Distribution ◽  
Virus Rna

scholarly journals Post-Epidemic Distribution of Schmallenberg Virus in Culicoides Arbovirus Vectors in Poland

Viruses ◽  
2019 ◽  
Vol 11 (5) ◽  
pp. 447 ◽  
Author(s):  
Julia Kęsik-Maliszewska ◽  
Magdalena Larska ◽  
Áine B. Collins ◽  
Jerzy Rola
Keyword(s):  
Infection Rate ◽  
Schmallenberg Virus ◽  
Simultaneous Increase ◽  
Virus Rna ◽  
Host Blood ◽  
First Time ◽  
The Relationship ◽  
Pooled Samples ◽  
Culicoides Vectors ◽  
Minimum Infection Rate

Pooled samples of female and male Culicoides midges (5146 and 332 pools, respectively) that corresponded to a total number of 124,957 specimens were collected between 2013–2017 in the vicinity of cattle barns that were distributed throughout Poland were analyzed for the presence of Schmallenberg virus (SBV) RNA. Sixty-six pools tested positive (1.2%) with mean Ct value of 34.95. The maximum likelihood estimated infection rate (MLE) was calculated at 0.53 per 1000 individuals; however, it peaked in 2016 with MLE of 3.7. Viral RNA was detected in C. obsoletus/scoticus complex, C. punctatus, and C. pulicaris pools. Moreover, viral material was present in nulliparous (virgin) Culicoides females (MLE 0.27) and for the first time reported in males (MLE 0.34), which suggests the possibility of transovarial route of SBV or virus RNA transmission, as both do not fed on host blood. The accuracy of targeted versus random SBV surveillance in Culicoides vectors was compared. The relationship between infection rate (expressed as minimum infection rate; MIR), in addition to MLE, was compared with the density of virus infected midges (DIM). In conclusion, the SBV infection rate in the vector was significantly higher in 2016 as compared to other surveillance years; this is consistent with the simultaneous increase in SBV seroprevalence (seroconversion) in ruminants during the same year.

scholarly journals Schmallenberg virus detection in bovine semen after experimental infection of bulls

2013 ◽  
Vol 142 (7) ◽  
pp. 1495-1500 ◽  
Author(s):  
W. H. M. VAN DER POEL ◽  
J. M. PARLEVLIET ◽  
E. R. A. M. VERSTRATEN ◽  
E. A. KOOI ◽  
R. HAKZE-VAN DER HONING ◽  
...  
Keyword(s):  
Cell Culture ◽  
Lymph Nodes ◽  
Experimental Infection ◽  
Virus Detection ◽  
Schmallenberg Virus ◽  
Post Inoculation ◽  
Pcr Assay ◽  
Blood Samples ◽  
Bovine Semen ◽  
Qrt Pcr

SUMMARYTo study Schmallenberg virus (SBV) excretion in bovine semen after experimental infection, two bulls were inoculated subcutaneously with a SBV isolate (1 ml Vero cell culture 106 TCID50). After inoculation (at day 0), semen was collected daily from both animals for 21 days and samples were tested for SBV by qRT–PCR assay. At 24 days post-inoculation both animals were subjected to necropsy and the genital organs and lymph nodes draining these organs were also tested for SBV RNA (qRT–PCR). After SBV infection both animals in the study showed viraemia (qRT–PCR) with fever and diarrhoea. SBV RNA could be detected in semen from both animals. The highest SBV RNA concentrations in semen were found in the first week (days 4–7 post-inoculation) but concentrations were relatively low (Ct values 30–39). Viable SBV was only isolated from blood samples and not from semen or genital tissues.

scholarly journals European interlaboratory comparison of Schmallenberg virus (SBV) real-time RT-PCR detection in experimental and field samples

2015 ◽  
Vol 27 (4) ◽  
pp. 422-430 ◽  
Author(s):  
Claudia Schulz ◽  
Wim H. M. van der Poel ◽  
Claire Ponsart ◽  
Ann Brigitte Cay ◽  
Falko Steinbach ◽  
...  
Keyword(s):  
Real Time ◽  
Extraction Methods ◽  
Diagnostic Sensitivity ◽  
Schmallenberg Virus ◽  
Detection Methods ◽  
Tissue Homogenate ◽  
Inhibitory Effects ◽  
Rna Detection ◽  
Bovine Semen ◽  
Field Samples

Molecular methods for the detection of Schmallenberg virus (SBV) RNA were rapidly developed after the emergence of this novel orthobunyavirus in Europe. The SBV epizootic wave has declined, but infectious SBV in SBV RNA–positive semen remains a possible risk for the distribution of SBV. However, the abilities of SBV molecular detection methods used at European laboratories have not yet been assessed, to our knowledge. The performances of extraction and real-time reverse transcription polymerase chain reaction (RT-qPCR) methods used at 27 German and 17 other European laboratories for SBV RNA detection in the matrices of whole blood, serum, tissue homogenate, RNA eluates, and bovine semen were evaluated in 2 interlaboratory trials with special emphasis on semen extraction methods. For reliable detection of viral genome in bovine semen samples, highly effective extraction methods are essential to cope with the potential inhibitory effects of semen components on PCR results. All methods used by the 44 laboratories were sufficiently robust to detect SBV RNA with high diagnostic sensitivity (100%) and specificity (95.8%) in all matrices, except semen. The trials demonstrated that the published recommended semen extraction methods (Hoffmann et al. 2013) and a combination of TRIzol LS with an alternative extraction kit have a considerably higher diagnostic sensitivity to detect SBV RNA in semen up to a detection limit of Cq ≤35 compared to other extraction methods used. A thorough validation of extraction methods with standardized semen batches is essential before their use for SBV RNA detection in bovine semen.

scholarly journals Infectious Schmallenberg Virus from Bovine Semen, Germany

2014 ◽  
Vol 20 (2) ◽  
pp. 337-339 ◽  
Author(s):  
Claudia Schulz ◽  
Kerstin Wernike ◽  
Martin Beer ◽  
Bernd Hoffmann
Keyword(s):  
Schmallenberg Virus ◽  
Bovine Semen

scholarly journals Quality controlled SARS-CoV-2 duplex procedure to reduce time and scarce molecular biology diagnosis reagents

2020 ◽  
Author(s):  
Anaelle Collin ◽  
Carole Chaboteaux ◽  
Veronique Fontaine ◽  
Philippe Lefevre
Keyword(s):  
Molecular Biology ◽  
Working Conditions ◽  
Internal Control ◽  
Internal Controls ◽  
Schmallenberg Virus ◽  
Routine Diagnosis ◽  
Virus Rna ◽  
One Step

The aim of this study was to provide validated procedures allowing to detect the SARS-CoV-2 and an internal control in a unique one-step duplex RT-qPCR. Two internal controls were tested, targeting either the Schmallenberg virus RNA provided by the NARILIS laboratory (University of Namur) with a HEX-labelled probe or a Diagenode Diagnostics internal control with a Cy5-labelled probe. Our results showed that Ct values of the RT-qPCR duplex assay were even smaller in the optimized working conditions, allowing to use the optimized qPCR conditions in routine diagnosis.

CME-Dermatologie 22/Antworten: Masern – Epidemiologie und Klinik

Praxis ◽  
2020 ◽  
Vol 109 (15) ◽  
pp. 1179-1181
Author(s):  
Luc Hari ◽  
Stephan Lautenschlager
Keyword(s):  
Virus Rna ◽  
Sind Eine

Zusammenfassung. Die Masern sind eine hoch ansteckende, akute und febrile Krankheit, die durch eine Infektion mit dem Masernvirus verursacht wird. Gemäss Schätzungen der Weltgesundheitsorganisation (WHO) kommt es bei einer weltweiten Prävalenz von zehn Millionen Patientinnen und Patienten pro Jahr zu rund 142 000 Todesfällen (Mortalitätsrate 1,4 %). Klinisch zeigt sich ein stadienhafter Verlauf: Drei bis vier Tage nach dem initialen katarrhalischen Prodromalstadium mit Fieber und Krankheitsgefühl bildet sich das charakteristische Exanthem aus. Die Diagnosestellung erfolgt primär klinisch, muss aber laborchemisch durch den Nachweis von spezifischen IgM-Antikörpern im Serum oder Virus-RNA im Rachenabstrich bestätigt werden. Die Therapie ist rein supportiv, eine spezifische antivirale Behandlung existiert nicht. Der Prävention kommt daher ein absolut zentraler Stellenwert zu, da es sich bei den Masern um eine durch Impfstoffe vermeidbare Erkrankung handelt.

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