The effect of 2-nitropropanol (2NPOH) administration on Salmonella enterica serovar Typhimurium in experimentally infected chicks was determined. Chicks orally challenged with 106 CFU/ml of a novobiocin- and naladixic acid–resistant Salmonella Typhimurium at 6 days of age were divided into three groups receiving 0 (control), 6.5, and 13 mg 2NPOH per bird (experiment 1) or four groups receiving 0 (control), 13, 65, and 130 mg 2NPOH per bird (experiment 2). Treatments were administered orally 1 day post–Salmonella challenge. Cecal contents collected at necropsy 24 and 48 h after treatment were subjected to bacterial and volatile fatty acid (VFA) analysis. In experiment 1, concentrations (mean ± SD log CFU per g) of Salmonella were reduced (P < 0.05) in the group administered 13 mg 2NPOH per bird at both the 24- and 48-h samplings compared with the controls (2.58 ± 2.10 versus 4.64 ± 1.79 and 2.88 ± 2.78 versus 5.03 ± 2.42 at 24 and 48 h, respectively). In experiment 2, mean ± SD populations of Salmonella were reduced (P < 0.05) in all groups receiving 2NPOH compared with untreated controls (3.65 ± 2.01, 3.39 ± 2.42, and 3.47 ± 1.55 at 13, 65, and 130 mg, respectively, versus 6.09 ± 1.02). Propionate concentrations were reduced (P < 0.05) by the 13-mg 2NPOH per bird treatment. Total VFA concentrations from the group treated with 13 mg 2NPOH per bird were lower (P < 0.05) by 48, but not 24, hours posttreatment than those from the group treated with 6.5 mg 2NPOH per bird. These results demonstrate the inhibitory activity of 2NPOH against Salmonella Typhimurium in vivo.
Effects of dietary clays on performance and intestinal mucus barrier of broiler chicks challenged with Salmonella enterica serovar Typhimurium and on goblet cell function in vitro
