Phylogeny of Mycoplasma bovis isolates from cattle and bison based on multi locus sequence typing and multiple-locus variable-number tandem repeats

Altogether, 369 pathogenic Yersinia enterocolitica isolates from 1,118 fecal samples collected from 22 pig farms of different production types were characterized by biotyping, serotyping, and genotyping using multiple-locus variable-number tandem repeats analysis. We investigated the distribution of the different genotypes at the farm level and their association with different farm conditions. Pigs were found to carry and transmit Y. enterocolitica between farms, because the same genotypes were found on farms that had previously transported the pigs between them. The purchase of new animals for the farms associated significantly with the number of different multiple-locus variable-number tandem repeats analysis types of Y. enterocolitica found within a farm. Some genotypes seemed to persist on farms for years. The results of this study show that pigs purchased from infected herds transmit Y. enterocolitica infection between farms. Certain pig farms may act as long-term sources of infection.

Download Full-text

Development of Multiple-Locus Variable-Number Tandem-Repeat Analysis for the Molecular Subtyping of Enterobacter sakazakii

Applied and Environmental Microbiology ◽

10.1128/aem.01726-07 ◽

2007 ◽

Vol 74 (4) ◽

pp. 1223-1231 ◽

Cited By ~ 25

Author(s):

N. R. Mullane ◽

M. Ryan ◽

C. Iversen ◽

M. Murphy ◽

P. O'Gaora ◽

...

Keyword(s):

Tandem Repeats ◽

Fluorescent Dyes ◽

Variable Number Tandem Repeat ◽

Variable Number ◽

Enterobacter Sakazakii ◽

Multiple Locus ◽

Epidemiological Tool ◽

Genescan Analysis ◽

Variable Number Tandem Repeats ◽

Multiple Locus Vntr Analysis

ABSTRACT The genomic content of Enterobacter sakazakii strain ATCC BAA-894 was analyzed for variable-number tandem repeats (VNTRs). In this study we report the development of a multiple-locus VNTR analysis (MLVA) strategy for the subtyping of E. sakazakii. The method is based on a GeneScan analysis of four VNTR loci labeled with multiple fluorescent dyes. This approach was applied to a collection of 112 isolates representing all 16 of the currently defined E. sakazakii biogroups. MLVA successfully discriminated among these isolates and compared favorably with pulsed-field gel electrophoresis. The method was relatively fast and easy to perform. The potential value of MLVA as an epidemiological tool is discussed.

Download Full-text

Rapid multiple-locus variable-number tandem-repeats analysis of Shigella spp. using multicolour capillary electrophoresis

Journal of Microbiological Methods ◽

10.1016/j.mimet.2010.10.006 ◽

2010 ◽

Vol 83 (3) ◽

pp. 279-285 ◽

Cited By ~ 6

Author(s):

Monica Rawal ◽

Elsebeth Hoff ◽

Lena Aas-Pedersen ◽

Kjersti Haugum ◽

Bjørn-Arne Lindstedt

Keyword(s):

Capillary Electrophoresis ◽

Tandem Repeats ◽

Variable Number ◽

Multiple Locus ◽

Shigella Spp ◽

Variable Number Tandem Repeats

Download Full-text

Análise genotípica do Helicobacter pylori pelo método Multiple-Locus Variable-Number Tandem-Repeats no sul do Brasil

Revista de Epidemiologia e Controle de Infecção ◽

10.17058/reci.v6i4.8043 ◽

2016 ◽

Vol 6 (4) ◽

Author(s):

Priscila Cristina Bartolomeu Halicki ◽

Ivy Bastos Ramis ◽

Júlia Silveira Vianna ◽

Thaísa Bozzetti Gautério ◽

Andrea von Groll ◽

...

Keyword(s):

Helicobacter Pylori ◽

In Silico ◽

Tandem Repeats ◽

Variable Number ◽

Multiple Locus ◽

H Pylori ◽

Variable Number Tandem Repeats

Justificativa e objetivos: Apesar de ser utilizado como um método de genotipagem para diferentes microrganismos, poucos estudos relatam a utilização de Multiple-Locus Variable Number of Tandem Repeats Analysis (MLVA) para análise da diversidade clonal do Helicobacter pylori. O objetivo deste estudo foi determinar a variabilidade genética de cepas de H. pylori pelo MLVA no sul do Brasil. Métodos: 95 amostras de DNA de H. pylori foram obtidas a partir de biópsias gástricas de pacientes H. pylori-positivos provenientes de duas cidades do sul do Brasil e a diversidade genética das cepas foi avaliada pelo método MLVA utilizando eletroforese em gel de agarose. Para a seleção dos loci a serem analisados neste estudo, foi realizada uma análise in silico de 12 loci previamente descritos na literatura. Resultados: A partir da análise in silico, apenas quatro loci foram considerados viáveis para a análise genotípica das cepas, resultando em 90 cepas distribuídas em oito grupos diferentes e cinco cepas órfãs. Conclusões: Apesar de o método MLVA permitir fazer inferências acerca da diversidade genética de uma população, nossos resultados mostraram que os métodos de genotipagem do H. pylori devem ser criticamente avaliados antes de serem utilizados nessa região do Brasil.

Download Full-text