Comparative evaluation of enzyme-linked immunosorbent assay based on crude and purified antigen in the diagnosis of canine visceral leishmaniasis in symptomatic and oligosymptomatic dogs

2008 ◽  
Vol 157 (3-4) ◽  
pp. 175-181 ◽  
Author(s):  
Teresinha Cristina Cândido ◽  
Sílvia Helena Venturoli Perri ◽  
Tatiana de Oliveira Gerzoschkwitz ◽  
Maria Cecília Rui Luvizotto ◽  
Valéria Marçal Felix de Lima
Keyword(s):  
Visceral Leishmaniasis ◽  
Immunosorbent Assay ◽  
Comparative Evaluation ◽  
Enzyme Linked Immunosorbent Assay ◽  
Canine Visceral Leishmaniasis ◽  
Purified Antigen

scholarly journals Dual-path platform (DPP) and enzyme-linked immunosorbent assay (ELISA): Change the sequence of the tests does not change the number of positive dogs for canine visceral leishmaniasis

2017 ◽  
Vol 11 (3) ◽  
pp. 106-109 ◽  
Author(s):  
Santos Almeida Sara ◽  
Lobo Gomes Carla ◽  
Costa Silva Elaynne ◽  
Tolentino Rocha Brandao Sarah ◽  
Patricia Aviz Wellida ◽  
...  
Keyword(s):  
Visceral Leishmaniasis ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Canine Visceral Leishmaniasis

scholarly journals A β-mercaptoethanol–modified enzyme-linked immunosorbent assay for diagnosis of canine visceral leishmaniasis

2013 ◽  
Vol 25 (2) ◽  
pp. 239-242
Author(s):  
Laura Barral-Veloso ◽  
Saul J. Semião-Santos ◽  
Paulo P. de Andrade ◽  
Marcia A. de Melo ◽  
Luís Martins ◽  
...  
Keyword(s):  
Visceral Leishmaniasis ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Canine Visceral Leishmaniasis ◽  
Modified Enzyme

Diagnosis of Canine Visceral Leishmaniasis with a Dot-Enzyme-Linked Immunosorbent Assay

1995 ◽  
Vol 53 (1) ◽  
pp. 40-42 ◽  
Author(s):  
Reynaldo Dietze ◽  
Luiz Carlos Pedrosa Valli ◽  
Temizio Pereira Rodriques ◽  
Aloisio Falqueto ◽  
Ralph Corey ◽  
...  
Keyword(s):  
Visceral Leishmaniasis ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Canine Visceral Leishmaniasis

scholarly journals Evaluation of a monoclonal antibody affinity purified antigen for zymodeme specific serological diagnosis of Trypanosoma cruzi infection

1987 ◽  
Vol 82 (1) ◽  
pp. 81-85 ◽  
Author(s):  
Mauro Schechter
Keyword(s):  
Monoclonal Antibody ◽  
Trypanosoma Cruzi ◽  
Affinity Chromatography ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Specific Monoclonal Antibody ◽  
Antibody Affinity ◽  
Specific Diagnosis ◽  
Purified Antigen ◽  
Cruzi Infection

Theoretically, serological assays with affinity purified marker antigens can allow strain-specific diagnosis even when parasites cannot be retrieved from and infected host. A Trypanosoma cruzi antigen was purified by affinity chromatography using a zymodeme (Z) 2 specific monoclonal antibody (2E2C11). An indirect enzyme-linked immunosorbent assay (ELISA) based on the purified antigen could discriminate between sera from rabbits immunized with T. cruzi zymodeme clones but could not discriminate between sera from mice infected with different zymodemes.

scholarly journals Diagnosis of Visceral Leishmaniasis by Enzyme-Linked Immunosorbent Assay Using Urine Samples

2002 ◽  
Vol 9 (4) ◽  
pp. 789-794 ◽  
Author(s):  
Mohammad Zahidul Islam ◽  
Makoto Itoh ◽  
S. M. Shamsuzzaman ◽  
Rusella Mirza ◽  
Farzana Matin ◽  
...  
Keyword(s):  
Visceral Leishmaniasis ◽  
Sensitivity And Specificity ◽  
Immunosorbent Assay ◽  
Leishmania Donovani ◽  
Laboratory Diagnosis ◽  
Urine Samples ◽  
Enzyme Linked Immunosorbent Assay ◽  
Healthy Controls ◽  
Serum Samples ◽  
Crude Antigen

ABSTRACT A diagnostic method has been developed to detect anti-Leishmania donovani immunoglobulin G (IgG) in urine by enzyme-linked immunosorbent assay (ELISA). In measuring anti-L. donovani IgG, IgA, and IgM in urine, the method performed best in the detection of IgG. The sensitivity and specificity of the assay were determined with panels of urine samples from 62 visceral leishmaniasis (VL) patients, 59 healthy controls from areas of endemicity, 53 healthy controls from areas of nonendemicity, 59 malaria patients, 13 tuberculosis patients, 23 cutaneous leishmaniasis patients, and 7 patients with other diseases. Using L. donovani promastigote crude antigen, the test had 93.5% sensitivity (58 positives of 62 VL patient samples) and 89.3% specificity (191 negatives of 214 non-VL patient samples). The ELISA with acetone-treated L. donovani promastigote antigen raised the sensitivity and specificity to 95.0 and 95.3%, respectively. Western blot analysis revealed that most of the samples that cross-reacted with crude antigen in ELISA did not recognize any antigenic component of L. donovani crude antigen. We also checked 40 serum samples from the same group of VL patients for anti-L. donovani IgG and got 90.0% sensitivity with both crude and acetone-treated antigens. As collection of urine is much easier than collection of serum, the detection of anti-L. donovani IgG in urine with acetone-treated antigen will be useful in epidemiological studies. It could be an adjunct of laboratory diagnosis.

scholarly journals Accuracy of Enzyme-Linked Immunosorbent Assay Using Crude and Purified Antigens for Serodiagnosis of Melioidosis

2006 ◽  
Vol 14 (1) ◽  
pp. 110-113 ◽  
Author(s):  
Narisara Chantratita ◽  
Vanaporn Wuthiekanun ◽  
Aunchalee Thanwisai ◽  
Direk Limmathurotsakul ◽  
Allen C. Cheng ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Diagnostic Tests ◽  
Burkholderia Pseudomallei ◽  
Enzyme Linked Immunosorbent Assay ◽  
Northeast Thailand ◽  
Hemagglutination Assay ◽  
Assay Sensitivity ◽  
Diagnostic Indices ◽  
Indirect Hemagglutination ◽  
Purified Antigen

ABSTRACT Five enzyme-linked immunosorbent assays developed to detect antibodies to different Burkholderia pseudomallei antigen preparations were evaluated as diagnostic tests for melioidosis in northeast Thailand. The highest diagnostic indices were observed for an affinity-purified antigen (sensitivity, 82%; specificity, 72%) and crude B. pseudomallei antigen (sensitivity, 81%; specificity, 70%), an improvement over the indirect hemagglutination assay (sensitivity, 73%; specificity, 64%).

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