scholarly journals Evaluation of a monoclonal antibody affinity purified antigen for zymodeme specific serological diagnosis of Trypanosoma cruzi infection

1987 ◽  
Vol 82 (1) ◽  
pp. 81-85 ◽  
Author(s):  
Mauro Schechter
Keyword(s):  
Monoclonal Antibody ◽  
Trypanosoma Cruzi ◽  
Affinity Chromatography ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Specific Monoclonal Antibody ◽  
Antibody Affinity ◽  
Specific Diagnosis ◽  
Purified Antigen ◽  
Cruzi Infection

Theoretically, serological assays with affinity purified marker antigens can allow strain-specific diagnosis even when parasites cannot be retrieved from and infected host. A Trypanosoma cruzi antigen was purified by affinity chromatography using a zymodeme (Z) 2 specific monoclonal antibody (2E2C11). An indirect enzyme-linked immunosorbent assay (ELISA) based on the purified antigen could discriminate between sera from rabbits immunized with T. cruzi zymodeme clones but could not discriminate between sera from mice infected with different zymodemes.

scholarly journals Epidemiological survey of Trypanosoma cruzi infection in North-Eastern Brazil using different diagnostic methods

1991 ◽  
Vol 33 (3) ◽  
pp. 193-198 ◽  
Author(s):  
José Figuerêdo-Silva ◽  
Yoshimasa Kaneda ◽  
Hiroshi Tachibana ◽  
Rieko Furushima ◽  
Seiki Tateno ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Trypanosoma Cruzi ◽  
Immunosorbent Assay ◽  
Epidemiological Survey ◽  
Diagnostic Methods ◽  
Enzyme Linked Immunosorbent Assay ◽  
Small Community ◽  
Eastern Brazil ◽  
North Eastern ◽  
Cruzi Infection

A survey of the prevalence of Trypanosoma cruzi infection was carried out in Oitis, a small community in the State of Piaui, Brazil. Two hundred and sixty five individuals were screened by microscopic examination, hémoculture, indirect immunofluorescence (IFA), enzyme-linked immunosorbent assay (ELISA), and competitive enzyme-linked immunosorbent assay (C-ELISA) using the monoclonal antibody TCF87 against to a 25kd T. cruzi antigen. Seropositivity was 14.3% by the IFA test, 14.7% by ELISA, and 13.2% by C-ELISA. The C-ELISA using the TCF87 monoclonal antibody seems to be applicable in serodiagnosis of Chagas' disease.

scholarly journals Evaluation of a chemiluminescent enzyme-linked immunosorbent assay for the diagnosis of Trypanosoma cruzi infection in a nonendemic setting

2013 ◽  
Vol 108 (7) ◽  
pp. 928-931 ◽  
Author(s):  
Luis Izquierdo ◽  
Alexandre Ferreira Marques ◽  
Montserrat Gállego ◽  
Sílvia Sanz ◽  
Sílvia Tebar ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Cruzi Infection

scholarly journals Sensitive quantitative analysis of the bitter glycoside amarogentin by specific monoclonal antibody-based indirect competitive enzyme-linked immunosorbent assay

RSC Advances ◽  
2018 ◽  
Vol 8 (31) ◽  
pp. 17410-17416 ◽  
Author(s):  
Seiichi Sakamoto ◽  
Shinji Wada ◽  
Hiroyuki Tanaka ◽  
Satoshi Morimoto
Keyword(s):  
Monoclonal Antibody ◽  
Quantitative Analysis ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Specific Monoclonal Antibody

MAb 1E9 was generated from AG–BSA conjugates possessing one AG molecule per BSA for icELISA.

scholarly journals Production of Group Specific Monoclonal Antibody to Aflatoxins and its Application to Enzyme-linked Immunosorbent Assay

2011 ◽  
Vol 27 (2) ◽  
pp. 125-131 ◽  
Author(s):  
Sung-Hee Kim ◽  
Sang-Ho Cha ◽  
Bischoff Karyn ◽  
Sung-Won Park ◽  
Seong-Wan Son ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Specific Monoclonal Antibody

Detection of Zearalenone By Tandem Immunoaffinity-Enzyme-Linked Immunosorbent Assay and Its Application to Milk

1990 ◽  
Vol 53 (7) ◽  
pp. 577-580 ◽  
Author(s):  
JUAN I. AZCONA ◽  
MOHAMED M. ABOUZIED ◽  
JAMES J. PESTKA
Keyword(s):  
Monoclonal Antibody ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Specific Monoclonal Antibody ◽  
Initial Sample ◽  
Average Recovery ◽  
Milk Samples ◽  
Coefficients Of Variation ◽  
Direct Elisa ◽  
Commercial Milk

A hybridoma-based method utilizing tandem affinity chromatography and enzyme-linked immunosorbent assay (ELISA) was devised to detect zearalenone. A zearalenone specific monoclonal antibody was attached to Sepharose for initial sample clean-up. Zearalenone was eluted with methanol and then quantified by competitive direct ELISA. Average ELISA recoveries from the column for water spiked with zearalenone at levels of 1, 5, 10, 25, and 50 ng/ml were 107, 86, 95, 95, and 92%, respectively, with a mean recovery of 95%. Mean interwell and interassay coefficients of variation were 9.7 and 8.9%, respectively. Average recovery by the method from milk spiked with zearalenone at levels of 1, 5, 10, 25, and 50 ng/ml was 187, 113, 107, 110, and 112%, respectively, with a mean recovery of 126%. Mean interwell and interassay coefficients of variation were 14.5 and 9.1%, respectively. Zearalenone was not detectable in 12 commercial milk samples assayed by the tandem method.

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