Detection of Leishmania infantum DNA by real-time PCR in canine oral and conjunctival swabs and comparison with other diagnostic techniques

2012 ◽  
Vol 184 (1) ◽  
pp. 10-17 ◽  
Author(s):  
Gabriella Lombardo ◽  
Maria Grazia Pennisi ◽  
Tiziana Lupo ◽  
Antonella Migliazzo ◽  
Alessandra Caprì ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Leishmania Infantum ◽  
Diagnostic Techniques

Reaktive Arthritis beim Hund nach Leishmanien-Infektion?

2008 ◽  
Vol 36 (01) ◽  
pp. 35-41
Author(s):  
D. Schaarschmidt ◽  
W. Müller ◽  
L. Walla ◽  
A. Borggräfe
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Leishmania Infantum ◽  
Reaktive Arthritis ◽  
Klinische Relevanz

Zusammenfassung: Gegenstand und Ziel: Erstbeschreibung einer möglicherweise leishmaniosebedingten Arthritis. Material und Methode: Fallbericht einer aus Griechenland importierten vierjährigen Mischlingshündin. Ergebnisse: Die Hündin wurde mit einer mittelgradigen Stützbeinlahmheit der rechten Vordergliedmaße vorgestellt. Radiologische, computertomographische Untersuchung sowie Arthroskopie ergaben eine mittelgradige Cubarthrose mit ausgeprägter Entknorpelung der Gelenkflächen. Serologisch wurde eine Leishmanioseinfektion diagnostiziert, wobei offensichtliche klinische Anzeichen einer viszeralen oder kutanen Leishmaniose fehlten. Mittels Real-Time-PCR ließ sich in der Synovia des betroffenen Ellbogengelenks Leishmaniainfantum-DNA nachweisen. Schlussfolgerung und klinische Relevanz: Bei Hunden mit Auslandsvorbericht und Arthropathien unklarer Genese bietet sich der direkte Nachweis von Leishmania-infantum-DNA mittels PCR aus Synovia an.

Detection of Leishmania infantum DNA by fret-based real-time PCR in urine from dogs with natural clinical leishmaniosis

2007 ◽  
Vol 147 (3-4) ◽  
pp. 315-319 ◽  
Author(s):  
L. Solano-Gallego ◽  
Alheli Rodriguez-Cortes ◽  
Michele Trotta ◽  
Claudia Zampieron ◽  
Luis Razia ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Leishmania Infantum

scholarly journals Data on the differentiation among Leishmania (Viannia) spp., Leishmania (Leishmania) infantum and Leishmania (Leishmania) amazonensis in Brazilian clinical samples using real-time PCR

Data in Brief ◽  
2020 ◽  
Vol 28 ◽  
pp. 104914 ◽  
Author(s):  
Aurora Diotallevi ◽  
Gloria Buffi ◽  
Marcello Ceccarelli ◽  
Herintha Coeto Neitzke-Abreu ◽  
Laisa Vieira Gnutzmann ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Leishmania Infantum ◽  
Leishmania Amazonensis ◽  
Clinical Samples

scholarly journals Quantification of Leishmania infantum DNA by a Real-Time PCR Assay with High Sensitivity

2004 ◽  
Vol 42 (11) ◽  
pp. 5249-5255 ◽  
Author(s):  
C. Mary ◽  
F. Faraut ◽  
L. Lascombe ◽  
H. Dumon
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Leishmania Infantum ◽  
High Sensitivity ◽  
Pcr Assay

scholarly journals The Mbam drainage system and onchocerciasis transmission post ivermectin mass drug administration (MDA) campaign, Cameroon

2021 ◽  
Vol 15 (1) ◽  
pp. e0008926
Author(s):  
Raphael Awah Abong ◽  
Glory Ngongeh Amambo ◽  
Ali Ahamat Hamid ◽  
Belinda Agbor Enow ◽  
Amuam Andrew Beng ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Large Scale ◽  
Water Discharge ◽  
Drainage System ◽  
River System ◽  
High Water ◽  
Diagnostic Techniques ◽  
Dry Seasons ◽  
The Impact

Background The impact of large scale Mass Drug Adminstration (MDA) of ivermectin on active onchocerciasis transmission by Simulium damnosum, which transmits the parasite O. volvulus is of great importance for onchocerciasis control programmes. We investigated in the Mbam river system area, the impact of MDA of ivermectin on entomological indices and also verify if there are river system factors that could have favoured the transmission of onchocerciasis in this area and contribute to the persistence of disease. We compared three independent techniques to detect Onchocerca larvae in blackflies and also analyzed the river system within 9 months post-MDA of ivermectin. Method Simulium flies were captured before and after 1, 3, 6 and 9months of ivermectin-MDA. The biting rate was determined and 41% of the flies dissected while the rest were grouped into pools of 100 flies for DNA extraction. The extracted DNA was then subjected to O-150 LAMP and real-time PCR for the detection of infection by Onchocerca species using pool screening. The river system was analysed and the water discharge compared between rainy and dry seasons. Principal findings We used human landing collection method (previously called human bait) to collect 22,274 adult female Simulium flies from Mbam River System. Of this number, 9,134 were dissected while 129 pools constituted for molecular screening. Overall biting and parous rates of 1113 flies/man/day and 24.7%, respectively, were observed. All diagnostic techniques detected similar rates of O. volvulus infection (P = 0.9252) and infectivity (P = 0.4825) at all monitoring time points. Onchocerca ochengi larvae were only detected in 2 of the 129 pools. Analysis of the river drainage revealed two hydroelectric dams constructed on the tributaries of the Mbam river were the key contributing factor to the high-water discharge during both rainy and dry seasons. Conclusion Results from fly dissection (Microscopy), real-time PCR and LAMP revealed the same trends pre- and post-MDA. The infection rate with animal Onchocerca sp was exceptionally low. The dense river system generate important breeding sites that govern the abundance of Simulium during both dry and rainy seasons.

Detection of Leishmania infantum kinetoplast minicircle DNA by Real Time PCR in hair of dogs with leishmaniosis

2013 ◽  
Vol 192 (1-3) ◽  
pp. 43-50 ◽  
Author(s):  
Silvia Belinchón-Lorenzo ◽  
Virginia Iniesta ◽  
Juan Carlos Parejo ◽  
Javier Fernández-Cotrina ◽  
Rubén Muñoz-Madrid ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Leishmania Infantum ◽  
Minicircle Dna

scholarly journals Parasite Burden in Hamsters Infected with Two Different Strains of Leishmania (Leishmania) infantum: “Leishman Donovan Units” versus Real-Time PCR

PLoS ONE ◽  
2012 ◽  
Vol 7 (10) ◽  
pp. e47907 ◽  
Author(s):  
Nádia das Dores Moreira ◽  
Juliana Vitoriano-Souza ◽  
Bruno Mendes Roatt ◽  
Paula Melo de Abreu Vieira ◽  
Henrique Gama Ker ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Leishmania Infantum ◽  
Parasite Burden ◽  
Different Strains

Epidemics of schistosomiasis in military staff assigned to endemic areas: standard diagnostic techniques and the development of real-time PCR techniques

2013 ◽  
Vol 23 (2) ◽  
pp. 226-227
Author(s):  
E. Biance-Valero ◽  
F. De Laval ◽  
M. Delerue ◽  
H. Savini ◽  
S. Cheinin ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Diagnostic Techniques ◽  
Endemic Areas ◽  
Pcr Techniques

scholarly journals Detection of Leishmania infantum in Lutzomyia longipalpis captured in Campo Grande, MS

2014 ◽  
Vol 23 (2) ◽  
pp. 269-273 ◽  
Author(s):  
Rodrigo Casquero Cunha ◽  
Renato Andreotti ◽  
Marlon Cezar Cominetti ◽  
Elaine Araújo Silva
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Urban Areas ◽  
Leishmania Infantum ◽  
Epidemiological Studies ◽  
Lutzomyia Longipalpis ◽  
Forest Reserves ◽  
Primary Vector ◽  
The City ◽  
Genus Leishmania

Leishmaniasis is a zoonotic disease caused by protozoa of the genus Leishmania (Ross, 1903) and is the focus of considerable attention in human and veterinary medicine. In the city of Campo Grande, MS, the causative agent of visceral leishmaniasis is Leishmania infantum (= L. chagasi) primary vector, comprising approximately 92.9% of the local sandfly population, is Lutzomyia longipalpis. The aim of this work was to compare real-time PCR with PCR as a tool for the detection of the kinetoplast DNA (kDNA) of L. infantum in sandflies. Sandflies of this species were caught, and a total of 38 samples with 1-4 individuals in each sample were obtained; these were distributed across 13 districts and divided between seven urban areas of the city of Campo Grande, MS. Three positive samples were found by PCR and, when using real-time PCR, this was able to detect the presence of this agent in 6 of the 13 districts sampled, which were all located on the outskirts of the city, where indicates the greater enzootic potential of these regions, as they are closer to natural forest reserves. We conclude that real-time PCR can be used for epidemiological studies of L. infantum.

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