scholarly journals Parasite Burden in Hamsters Infected with Two Different Strains of Leishmania (Leishmania) infantum: “Leishman Donovan Units” versus Real-Time PCR

PLoS ONE ◽  
2012 ◽  
Vol 7 (10) ◽  
pp. e47907 ◽  
Author(s):  
Nádia das Dores Moreira ◽  
Juliana Vitoriano-Souza ◽  
Bruno Mendes Roatt ◽  
Paula Melo de Abreu Vieira ◽  
Henrique Gama Ker ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Leishmania Infantum ◽  
Parasite Burden ◽  
Different Strains

Detection of Leishmania infantum DNA by real-time PCR in canine oral and conjunctival swabs and comparison with other diagnostic techniques

2012 ◽  
Vol 184 (1) ◽  
pp. 10-17 ◽  
Author(s):  
Gabriella Lombardo ◽  
Maria Grazia Pennisi ◽  
Tiziana Lupo ◽  
Antonella Migliazzo ◽  
Alessandra Caprì ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Leishmania Infantum ◽  
Diagnostic Techniques

Reaktive Arthritis beim Hund nach Leishmanien-Infektion?

2008 ◽  
Vol 36 (01) ◽  
pp. 35-41
Author(s):  
D. Schaarschmidt ◽  
W. Müller ◽  
L. Walla ◽  
A. Borggräfe
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Leishmania Infantum ◽  
Reaktive Arthritis ◽  
Klinische Relevanz

Zusammenfassung: Gegenstand und Ziel: Erstbeschreibung einer möglicherweise leishmaniosebedingten Arthritis. Material und Methode: Fallbericht einer aus Griechenland importierten vierjährigen Mischlingshündin. Ergebnisse: Die Hündin wurde mit einer mittelgradigen Stützbeinlahmheit der rechten Vordergliedmaße vorgestellt. Radiologische, computertomographische Untersuchung sowie Arthroskopie ergaben eine mittelgradige Cubarthrose mit ausgeprägter Entknorpelung der Gelenkflächen. Serologisch wurde eine Leishmanioseinfektion diagnostiziert, wobei offensichtliche klinische Anzeichen einer viszeralen oder kutanen Leishmaniose fehlten. Mittels Real-Time-PCR ließ sich in der Synovia des betroffenen Ellbogengelenks Leishmaniainfantum-DNA nachweisen. Schlussfolgerung und klinische Relevanz: Bei Hunden mit Auslandsvorbericht und Arthropathien unklarer Genese bietet sich der direkte Nachweis von Leishmania-infantum-DNA mittels PCR aus Synovia an.

Detection of Leishmania infantum DNA by fret-based real-time PCR in urine from dogs with natural clinical leishmaniosis

2007 ◽  
Vol 147 (3-4) ◽  
pp. 315-319 ◽  
Author(s):  
L. Solano-Gallego ◽  
Alheli Rodriguez-Cortes ◽  
Michele Trotta ◽  
Claudia Zampieron ◽  
Luis Razia ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Leishmania Infantum

scholarly journals Data on the differentiation among Leishmania (Viannia) spp., Leishmania (Leishmania) infantum and Leishmania (Leishmania) amazonensis in Brazilian clinical samples using real-time PCR

Data in Brief ◽  
2020 ◽  
Vol 28 ◽  
pp. 104914 ◽  
Author(s):  
Aurora Diotallevi ◽  
Gloria Buffi ◽  
Marcello Ceccarelli ◽  
Herintha Coeto Neitzke-Abreu ◽  
Laisa Vieira Gnutzmann ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Leishmania Infantum ◽  
Leishmania Amazonensis ◽  
Clinical Samples

scholarly journals Quantification of Leishmania infantum DNA by a Real-Time PCR Assay with High Sensitivity

2004 ◽  
Vol 42 (11) ◽  
pp. 5249-5255 ◽  
Author(s):  
C. Mary ◽  
F. Faraut ◽  
L. Lascombe ◽  
H. Dumon
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Leishmania Infantum ◽  
High Sensitivity ◽  
Pcr Assay

Detection of Leishmania infantum kinetoplast minicircle DNA by Real Time PCR in hair of dogs with leishmaniosis

2013 ◽  
Vol 192 (1-3) ◽  
pp. 43-50 ◽  
Author(s):  
Silvia Belinchón-Lorenzo ◽  
Virginia Iniesta ◽  
Juan Carlos Parejo ◽  
Javier Fernández-Cotrina ◽  
Rubén Muñoz-Madrid ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Leishmania Infantum ◽  
Minicircle Dna

scholarly journals Detection of Leishmania infantum in Lutzomyia longipalpis captured in Campo Grande, MS

2014 ◽  
Vol 23 (2) ◽  
pp. 269-273 ◽  
Author(s):  
Rodrigo Casquero Cunha ◽  
Renato Andreotti ◽  
Marlon Cezar Cominetti ◽  
Elaine Araújo Silva
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Urban Areas ◽  
Leishmania Infantum ◽  
Epidemiological Studies ◽  
Lutzomyia Longipalpis ◽  
Forest Reserves ◽  
Primary Vector ◽  
The City ◽  
Genus Leishmania

Leishmaniasis is a zoonotic disease caused by protozoa of the genus Leishmania (Ross, 1903) and is the focus of considerable attention in human and veterinary medicine. In the city of Campo Grande, MS, the causative agent of visceral leishmaniasis is Leishmania infantum (= L. chagasi) primary vector, comprising approximately 92.9% of the local sandfly population, is Lutzomyia longipalpis. The aim of this work was to compare real-time PCR with PCR as a tool for the detection of the kinetoplast DNA (kDNA) of L. infantum in sandflies. Sandflies of this species were caught, and a total of 38 samples with 1-4 individuals in each sample were obtained; these were distributed across 13 districts and divided between seven urban areas of the city of Campo Grande, MS. Three positive samples were found by PCR and, when using real-time PCR, this was able to detect the presence of this agent in 6 of the 13 districts sampled, which were all located on the outskirts of the city, where indicates the greater enzootic potential of these regions, as they are closer to natural forest reserves. We conclude that real-time PCR can be used for epidemiological studies of L. infantum.

Development of a minor groove binding probe based real-time PCR for the diagnosis and quantification of Leishmania infantum in dog specimens

2011 ◽  
Vol 91 (2) ◽  
pp. 243-245 ◽  
Author(s):  
Elena Galletti ◽  
Paolo Bonilauri ◽  
Lia Bardasi ◽  
Maria Cristina Fontana ◽  
Mattia Ramini ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Leishmania Infantum ◽  
Minor Groove ◽  
Minor Groove Binding ◽  
Groove Binding ◽  
A Minor

Real time PCR detection of rabbit haemorrhagic disease virus in rabbits infected with different European strains of RHDV

2013 ◽  
Vol 16 (1) ◽  
pp. 39-43 ◽  
Author(s):  
P. Niedźwiedzka-Rystwej ◽  
B. Hukowska-Szematowicz ◽  
J. Działo ◽  
B. Tokarz-Deptuła ◽  
W. Deptuła
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Viral Agent ◽  
Rabbit Haemorrhagic Disease Virus ◽  
European Regions ◽  
Biological Features ◽  
Dna And Rna ◽  
Rabbit Haemorrhagic Disease ◽  
Different Strains ◽  
Haemorrhagic Disease

Abstract The paper concerns the use of a novel, very effective diagnostic method, a real-time PCR for diagnosis of a viral agent causing viral haemorrhagic disease in rabbits - RHDV. Until now, the method was widely used for detecting many different viruses, both DNA, and RNA, but as far as RHDV is concerned, there are not many records of such use. This study aimed at the detection of 17 different strains from different European regions, differing in biological features and mortality. The study confirmed that real-time PCR is an applicable and effective method for diagnosis of RHDV, irrespective of the stains’ features.

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