Fasciola hepatica: Histological changes in the somatic and reproductive tissues of liver fluke following closantel treatment of experimentally-infected sheep

2016 ◽  
Vol 215 ◽  
pp. 38-47 ◽  
Author(s):  
S. Scarcella ◽  
R.E.B. Hanna ◽  
G.P. Brennan ◽  
H. Solana ◽  
I. Fairweather
2006 ◽  
Vol 100 (3) ◽  
pp. 461-465 ◽  
Author(s):  
Yazmin Alcala-Canto ◽  
Froylan Ibarra-Velarde ◽  
Hector Sumano-Lopez ◽  
Jesus Gracia-Mora ◽  
Aldo Alberti-Navarro

2021 ◽  
Vol 120 (3) ◽  
pp. 979-991
Author(s):  
Rebekah B. Stuart ◽  
Suzanne Zwaanswijk ◽  
Neil D. MacKintosh ◽  
Boontarikaan Witikornkul ◽  
Peter M. Brophy ◽  
...  

AbstractFasciola hepatica (liver fluke), a significant threat to food security, causes global economic loss for the livestock industry and is re-emerging as a foodborne disease of humans. In the absence of vaccines, treatment control is by anthelmintics; with only triclabendazole (TCBZ) currently effective against all stages of F. hepatica in livestock and humans. There is widespread resistance to TCBZ and its detoxification by flukes might contribute to the mechanism. However, there is limited phase I capacity in adult parasitic helminths with the phase II detoxification system dominated by the soluble glutathione transferase (GST) superfamily. Previous proteomic studies have demonstrated that the levels of Mu class GST from pooled F. hepatica parasites respond under TCBZ-sulphoxide (TCBZ-SO) challenge during in vitro culture ex-host. We have extended this finding by exploiting a sub-proteomic lead strategy to measure the change in the total soluble GST profile (GST-ome) of individual TCBZ-susceptible F. hepatica on TCBZ-SO-exposure in vitro culture. TCBZ-SO exposure demonstrated differential abundance of FhGST-Mu29 and FhGST-Mu26 following affinity purification using both GSH and S-hexyl GSH affinity. Furthermore, a low or weak affinity matrix interacting Mu class GST (FhGST-Mu5) has been identified and recombinantly expressed and represents a new low-affinity Mu class GST. Low-affinity GST isoforms within the GST-ome was not restricted to FhGST-Mu5 with a second likely low-affinity sigma class GST (FhGST-S2) uncovered. This study represents the most complete Fasciola GST-ome generated to date and has supported the potential of subproteomic analyses on individual adult flukes.


2021 ◽  
Vol 293 ◽  
pp. 109427
Author(s):  
Jane Lamb ◽  
Emma Doyle ◽  
Jamie Barwick ◽  
Michael Chambers ◽  
Lewis Kahn

Nature ◽  
1963 ◽  
Vol 198 (4876) ◽  
pp. 204-204 ◽  
Author(s):  
D. TEODOROVIĆ ◽  
I. BERKEŠ ◽  
M. MILOVANAVIĆ

Parasitology ◽  
1990 ◽  
Vol 101 (3) ◽  
pp. 395-407 ◽  
Author(s):  
A. W. Stitt ◽  
I. Fairweather

SUMMARYSpermatogenesis and the fine structure of the mature spermatozoon of Fasciola hepatica have been studied by transmission electron microscopy. The primary spermatogonia display a typical gonial morphology and occupy the periphery of the testis. They undergo 3 mitotic divisions to give rise to 8 primary spermatocytes forming a rosette of cells connected to a central cytophore. The primary spermatocytes undergo 2 meiotic divisions, resulting in 32 spermatids that develop into spermatozoa. Intranuclear synaptonemal complexes in primary spermatocytes confirm the first meiotic division. The onset of spermiogenesis is marked by the formation of the zone of differentiation which contains 2 basal bodies and a further centriole derivative, the central body. The zone extends away from the spermatid cell to form the median process; into this migrates the differentiated and elongate nucleus. Simultaneously, 2 axonemes develop from the basal bodies. During development, they rotate through 90° to extend parallel to the median process. The migration of the nucleus to the distal end of the median process coincides with the fusion of the axonemes to the latter to form a monopartite spermatozoon. The mature spermatozoon possesses 2 axonemes of the 9 + ‘1’ pattern typical of parasitic platyhelminths, 2 elongate mitochondria and a variable array of peripheral microtubules. The nuclear region of the spermatozoon is immotile. The value of sperm ultrastructure as a taxonomic tool in platyhelminth phylogeny is discussed.


2021 ◽  
pp. 109524
Author(s):  
Tania Rojas-Campos ◽  
Froylán Ibarra-Velarde ◽  
Yolanda Vera-Montenegro ◽  
Miguel Flores-Ramos ◽  
Irene Cruz-Mendoza ◽  
...  

Sign in / Sign up

Export Citation Format

Share Document