sperm ultrastructure
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2022 ◽  
Vol 66 ◽  
pp. 101129
Author(s):  
Glenda Dias ◽  
José Lino-Neto ◽  
David Mercati ◽  
Pietro Paolo Fanciulli ◽  
Pietro Lupetti ◽  
...  
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Aquaculture ◽  
2022 ◽  
Vol 547 ◽  
pp. 737471
Author(s):  
Leydy Sandoval-Vargas ◽  
Jennie Risopatrón ◽  
Kelly Dumorne ◽  
Jorge Farías ◽  
Elías Figueroa ◽  
...  

2021 ◽  
pp. 104780
Author(s):  
Sara Fikry Fouda ◽  
Ali Ali El-Raghi ◽  
Abdel-Khalek E. Abdel-Khalek ◽  
Mahmoud A. Hassan ◽  
Ibrahim Talat El-Ratel

2021 ◽  
Vol 64 ◽  
pp. 101084
Author(s):  
Stephanie Krueger ◽  
Gerald Moritz
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2021 ◽  
Author(s):  
Bryan Blawut ◽  
Barbara Wolfe ◽  
Christoper Premanandan ◽  
Gustavo Schuenemann ◽  
Stuart A. Ludsin ◽  
...  

Micron ◽  
2021 ◽  
pp. 103111
Author(s):  
Glenda Dias ◽  
José Lino-Neto ◽  
David Mercati ◽  
Pietro Paolo Fanciulli ◽  
Pietro Lupetti ◽  
...  
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Author(s):  
Denis Korneev ◽  
D. Jo Merriner ◽  
Gediminas Gervinskas ◽  
Alex de Marco ◽  
Moira K. O’Bryan

The analysis of spermatozoa morphology is fundamental to understand male fertility and the etiology of infertility. Traditionally scanning electron microscopy (SEM) has been used to define surface topology. Recently, however, it has become a critical tool for three-dimensional analysis of internal cellular ultrastructure. Modern SEM provides nanometer-scale resolution, but the meaningfulness of such information is proportional to the quality of the sample preservation. In this study, we demonstrate that sperm quickly and robustly adhere to gold-coated surfaces. Leveraging this property, we developed three step-by-step protocols fulfilling different needs for sperm imaging: chemically fixed monolayers for SEM examination of the external morphology, and two high-pressure freezing-based protocols for fast SEM examination of full cell internal morphology and focused ion-beam SEM tomography. These analyses allow previously unappreciated insights into mouse sperm ultrastructure, including the identification of novel structures within the fibrous sheath and domain-specific interactions between the plasma membrane and exosome-like structures.


2020 ◽  
Author(s):  
Elena V. Soldatenko ◽  
Andrey B. Shatrov ◽  
Anatoly A. Petrov ◽  
Alena A. Shirokaya
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