Review on Prevalence of Haemonchus Contort us in Ethiopian

In the 17th century, Robert Hooke invented the microscope and our life understanding and disease increased. Further understanding of the invisible, microscopic world has been occurred after the establishment of modern microbiology in the 19th century by Louis Pasteur and Robert Koch. Thanks to modern technology, there have been nearly 300 species of parasitic helminths known to be human parasite. Additionally, livestock, crops and pets are all victims of parasitic helminths, which cause extreme effects on the human population as well [1].

Parasitic helminths in snakes from the global legal trade

Summary In recent years, the demand for snakes imported from different countries around the world has increased in Slovakia. However, such snakes can be infected with a wide variety of parasites. We have been focused on monitoring the prevalence of parasitic helminths of snakes imported to Slovakia. From 2015 to 2020, 205 samples were collected. We examined faecal samples of 185 live snakes, and a parasitological autopsy was performed on 20 carcasses. Out of a total of 205 snakes, parasitic helminths were found in 44 individuals (21.46 %). Coprological examination of live snakes confirmed positivity for the presence of helminths in 38 snakes (20.54 %). Through parasitological autopsy of all the dead snakes, we found helminths or their eggs in 6 carcasses (30.00 %). The genera of helminths found in the positive snakes were: Ophiotaenia spp., Kapsulotaenia spp., Strongyloides spp., Rhabdias spp., Ophidascaris spp., Ascaridia spp., f. Heterakidae, Kalicephalus spp., Capillaria spp., order Oxyurida and stages of the plerocercoid of an unidentifi ed species of tapeworm in the subcutaneous tissue.

Automated Diagnostics: Advances in the Diagnosis of Intestinal Parasitic Infections in Humans and Animals

The increasingly close proximity between people and animals is of great concern for public health, given the risk of exposure to infectious diseases transmitted through animals, which are carriers of more than 60 zoonotic agents. These diseases, which are included in the list of Neglected Tropical Diseases, cause losses in countries with tropical and subtropical climates, and in regions with temperate climates. Indeed, they affect more than a billion people around the world, a large proportion of which are infected by one or more parasitic helminths, causing annual losses of billions of dollars. Several studies are being conducted in search for differentiated, more sensitive diagnostics with fewer errors. These studies, which involve the automated examination of intestinal parasites, still face challenges that must be overcome in order to ensure the proper identification of parasites. This includes a protocol that allows for elimination of most of the debris in samples, satisfactory staining of parasite structures, and a robust image database. Our objective here is therefore to offer a critical description of the techniques currently in use for the automated diagnosis of intestinal parasites in fecal samples, as well as advances in these techniques.

Parasite load of raw wastewater from the city of Biskra’s discharge outfalls - Z’mor and Biskra valleys (Algeria)

Algeria, like other arid and semi-arid African countries, is confronted by problems related to rapid population growth and water stress (water short-age). This stress fosters raw wastewater reuse in market gardening and hor-ticulture. The advantages of this wastewater consist of their nutrients whose organic materials contribute to soils enrichment with humus, as well as their availability in large quantities. However, raw wastewater reuse entails a po-tential risk of environmental and water resources pollution. The purpose of this work was to evaluate the parasite load of raw wastewater from the city of Biskra’s discharge outfalls (Biskra valley and Z'mor valley), while consider-ing the demographic and socio-economic profile of populations connected to these outfalls. Our results revealed a variety of parasites in the form of hel-minths eggs at the following average concentrations: Ascaris sp. (33.66 eggs/L,), Trichuris sp. (22 eggs/L), Enterobius vermicularis (19.58 eggs/L), Ancy-lostoma sp. (17.41 eggs/L), Nematodirus sp. 17.83 (eggs/L), Hymenolepis nana (21.66 eggs/L), Moniezia expansa (16.5 eggs/L), Taenia sp. (18.5 eggs/L), Fasciola hepatica (0.58 eggs/L) and Strongyloides spp (17.11 eggs/L). The parasitic helminths eggs distribution varies according to the size of urban areas and the socio-economic level of populations connected to each outfall. This study also highlighted the qualitative and quantitative seasonal varia-tions of helminths eggs in wastewater from Biskra’s two outfalls.

Transcriptomic analyses implicate neuronal plasticity and chloride homeostasis in ivermectin resistance and recovery in a parasitic nematode

The antiparasitic drug ivermectin plays an essential role in human and animal health globally. However, ivermectin resistance is widespread in veterinary helminths and there are growing concerns of sub-optimal responses to treatment in related helminths of humans. Despite decades of research, the genetic mechanisms underlying ivermectin resistance are poorly understood in parasitic helminths. This reflects significant uncertainty regarding the mode of action of ivermectin in parasitic helminths, and the genetic complexity of these organisms; parasitic helminths have large, rapidly evolving genomes and differences in evolutionary history and genetic background can confound comparisons between resistant and susceptible populations. We undertook a controlled genetic cross of a multi-drug resistant and a susceptible reference isolate of Haemonchus contortus, an economically important gastrointestinal nematode of sheep, and ivermectin-selected the F2 population for comparison with an untreated F2 control. RNA-seq analyses of male and female adults of all populations identified high transcriptomic differentiation between parental isolates, which was significantly reduced in the F2, allowing differences associated specifically with ivermectin resistance to be identified. In all resistant populations, there was constitutive upregulation of a single gene, HCON_00155390:cky-1, a putative pharyngeal-expressed transcription factor, in a narrow locus on chromosome V previously shown to be under ivermectin selection. In addition, we detected sex-specific differences in gene expression between resistant and susceptible populations, including constitutive upregulation of a P-glycoprotein, HCON_00162780:pgp-11, in resistant males only. After ivermectin selection, we identified differential expression of genes with roles in neuronal function and chloride homeostasis, which is consistent with an adaptive response to ivermectin-induced hyperpolarisation of neuromuscular cells. Overall, we show the utility of a genetic cross to identify differences in gene expression that are specific to ivermectin selection and provide a framework to better understand ivermectin resistance and recovery in parasitic helminths.

First Record of Two Parasitic Helminths from Cobia Rachycentron canadum (L.) (Carangiformes, Rachycentridae) from Iraqi Marine Waters

A total of 14 specimens of Cobia Rachycentron canadum (L.) were caught from Iraqi marine waters during the period from October 2019 till May 2020. Two parasitic helminths (One species each of nematode and acanthocephalan) were isolated from alimentary canal of the cobia. These are the adults of Iheringascaris inquies (Linton, 1901) Deardorff & Overstreet, 1980 and Serrasentis sagittifer Linton, 1889. The record of these worms, from this fish, is considered as the first record in Iraq. Adequate description of both I. inquies and adult S. sagittifer from cobia for the first time in the Arabian Gulf is given.

Analysis of Schistosoma mansoni Extracellular Vesicles Surface Glycans Reveals Potential Immune Evasion Mechanism and New Insights on Their Origins of Biogenesis

Parasitic helminths are master manipulators of host immunity. Their strategy is complex and involves the release of excreted/secreted products, including extracellular vesicles (EVs). The protein and miRNA contents of EVs have been characterised for many parasitic helminths but, despite reports suggesting the importance of EV surface carbohydrate structures (glycans) in the interactions with target cells and thus subsequent effector functions, little is known about parasite EV glycomics. Using lectin microarrays, we identified several lectins that exhibit strong adhesion to Schistosoma mansoni EVs, suggesting the presence of multiple glycan structures on these vesicles. Interestingly, SNA-I, a lectin that recognises structures with terminal sialic acid, displayed strong affinity for S. mansoni EVs, which was completely abolished by neuraminidase treatment, suggesting sialylation in the EV sample. This finding is of interest, as sialic acids play important roles in the context of infection by aiding immune evasion, affecting target recognition, cell entry, etc., but are not thought to be synthesised by helminths. These data were validated by quantitative analysis of free sialic acid released from EVs following treatment with neuraminidase. Lectin histochemistry and fluorescence in situ hybridisation analyses on whole adult worms suggest the involvement of sub-tegumental cell bodies, as well as the digestive and excretory systems, in the release of EVs. These results support previous reports of EV biogenesis diversity in trematodes and potentially highlight new means of immune modulation and evasion employed by schistosomes.

Direct reprogramming of the intestinal epithelium by parasitic helminths subverts type 2 immunity

Enteric helminths form intimate physical connections with the intestinal epithelium, yet their ability to directly alter epithelial stem cell fate has not been resolved. Here we demonstrate that infection of mice with the symbiotic parasite Heligmosomoides polygyrus bakeri (Hbp), reprograms the intestinal epithelium into a fetal-like state marked by the emergence of Clusterin-expressing revival stem cells (revSCs). Organoid-based studies using parasite-derived excretory/secretory products reveal that Hpb-mediated revSC generation occurs independent of host-derived immune signals and inhibits type 2 cytokine-driven differentiation of secretory epithelial lineages that promote their expulsion. Reciprocally, type 2 cytokine signals limit revSC differentiation and, consequently, Hpb fitness indicating that helminths compete with their host for control of the intestinal stem cell niche to promote continuation of their life cycle.

Gastrointestinal Helminthic Parasites of Stray Cats (Felis catus) in Northwest Iran

Background: Stray cats are considered an important source of various human and animal diseases, particularly diseases of parasitic helminths. We aimed to investigate the distribution of zoonotic species of gastrointestinal helminths in stray cats in Meshkin-Shahr district in Ardabil Province in the northwest of Iran. Methods: The gastrointestinal tract of 104 stray cats from villages of Meshkin-Shahr district were provided during 2014-2015. Each gastrointestinal tract was cut into distinct sections, including esophagus, stomach, small intestine, and large intestine, and each section was examined separately for detection of helminths. Helminths were collected and then identified at the species level after clearing and staining. Results: Overall, 88 out of 104 cats (84.6%) were found to be infected with at least one gastrointestinal helminth. The rate of infection for each species was as follows: Toxocara mystax (syn. cati) (49%), Taenia taeniaeformis (44.2%), Joyexiella pasqualei (32.7%), Dipylidium caninum (23.1%), Rictularia cahirensis (4.8%), and Physaloptera praeputialis (4.8%). Among these parasites, only Ph. praeputialis was collected from the stomach, all other helminths were collected from the small intestine. Conclusion: The results demonstrate a high infection rate of stray cats with zoonotic helminths. The presence of zoonotic species in stray cats, particularly T. mystax, has public health importance.