One single nucleotide difference alters the differential expression of spliced RNAs between HBV genotypes A and D

2013 ◽  
Vol 174 (1-2) ◽  
pp. 18-26 ◽  
Author(s):  
Chien-Chiao Huang ◽  
Tzer-Min Kuo ◽  
Chau-Ting Yeh ◽  
Cheng-po Hu ◽  
Ya-Ling Chen ◽  
...  
Keyword(s):  
Differential Expression ◽  
Nucleotide Difference ◽  
Single Nucleotide ◽  
Hbv Genotypes

scholarly journals Isolation of Salmonella enterica subspecies enterica serovar Paratyphi B dT+, or Salmonella Java, from Indonesia and alteration of the d-tartrate fermentation phenotype by disrupting the ORF STM 3356

2006 ◽  
Vol 55 (12) ◽  
pp. 1661-1665 ◽  
Author(s):  
Kyung Ho Han ◽  
Seon Young Choi ◽  
Je Hee Lee ◽  
Hyejon Lee ◽  
Eun Hee Shin ◽  
...  
Keyword(s):  
Salmonella Enterica ◽  
Reference Strain ◽  
Multidrug Resistant ◽  
Nucleotide Difference ◽  
Single Nucleotide ◽  
Functional Product ◽  
Paratyphi B ◽  
In Trans ◽  
Paratyphoid Fever ◽  
Discrimination Method

Salmonella enterica subspecies enterica serovar Paratyphi B [O1,4,(5),12 : Hb : 1,2] can cause either an enteric fever (paratyphoid fever) or self-limiting gastroenteritis in humans. The d-tartrate non-fermenting variant S. enterica subsp. enterica serovar Paratyphi B dT− (S. Paratyphi B) is the causative agent of paratyphoid fever, and the d-tartrate fermenting variant S. enterica subsp. enterica serovar Paratyphi B dT+ (S. Paratyphi B dT+; formerly called Salmonella Java) causes gastroenteritis. S. Java is currently recognized as an emerging problem worldwide. Twelve dT+ S. Java isolates were collected in Indonesia between 2000 and 2002. One-third of them contained Salmonella genomic island 1 (SGI1), which gives the multidrug-resistant phenotype to the bacteria. In this study, a PCR-based method to detect a single nucleotide difference responsible for the inability to ferment d-tartrate, reported elsewhere, was validated. The d-tartrate fermenting phenotype of S. Java was converted to the non-fermenting phenotype by the disruption of the ORF STM 3356, and the d-tartrate non-fermenting phenotype of the ORF STM 3356-disrupted strain and the dT− reference strain was changed to the dT+ phenotype by complementing ORF STM 3356 in trans. The results show that the dT+ phenotype requires a functional product encoded by STM 3356, and support the use of the PCR-based discrimination method for S. Paratyphi B and S. Java as the standard differentiation method.

Ultrasensitive SERS detection of highly homologous miRNAs by generating 3D organic-nanoclusters and a functionalized chip with locked nucleic acid probes

2018 ◽  
Vol 54 (95) ◽  
pp. 13431-13434 ◽  
Author(s):  
Mengting Zhu ◽  
Zhaomei Sun ◽  
Zhen Zhang ◽  
Shusheng Zhang
Keyword(s):  
Nucleic Acid ◽  
Locked Nucleic Acid ◽  
Nucleotide Difference ◽  
Single Nucleotide ◽  
Nucleic Acid Probes ◽  
Sers Detection ◽  
New Finding

Herein, a new finding is reported that 3D organic-nanoclusters (3DONs) with superior SERS properties as an original reporter could accurately and sensitively distinguish microRNAs (miRNAs) with highly similar sequences, even with a single-nucleotide difference.

A Peptide Nucleic Acid (PNA) Heteroduplex Probe Containing an Inosine-Cytosine Base Pair Discriminates a Single-Nucleotide Difference in RNA

2013 ◽  
Vol 19 (16) ◽  
pp. 5034-5040 ◽  
Author(s):  
Katsuhiko Matsumoto ◽  
Eiji Nakata ◽  
Tomoki Tamura ◽  
Isao Saito ◽  
Yasunori Aizawa ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Base Pair ◽  
Peptide Nucleic Acid ◽  
Nucleotide Difference ◽  
Single Nucleotide

scholarly journals Tunable Blinking Kinetics of Cy5 for Precise DNA Quantification and Single-Nucleotide Difference Detection

2008 ◽  
Vol 95 (2) ◽  
pp. 729-737 ◽  
Author(s):  
Hsin-Chih Yeh ◽  
Christopher M. Puleo ◽  
Yi-Ping Ho ◽  
Vasudev J. Bailey ◽  
Teck Chuan Lim ◽  
...  
Keyword(s):  
Dna Quantification ◽  
Nucleotide Difference ◽  
Single Nucleotide ◽  
Difference Detection ◽  
Kinetics Of
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