Construction of stable infectious full-length and eGFP-tagged cDNA clones of Mirabilis crinkle mosaic virus via In-Fusion cloning

Abstract Perilla is an annual herb with a unique aroma and taste and has been cultivated in Korea for hundreds of years. Owing to the highly edible and medicinal value of Perilla plants, it has been widely cultivated in many Asian and European countries. Recently, several viruses have been reported to cause diseases in Perilla in Korea, including turnip mosaic virus (TuMV) which is known as a brassica pathogen due to its significant damage to brassica crops. In this study, we determined the complete genome sequences of two new TuMV isolates originating from Perilla in Korea. Full-length infectious cDNA clones of these two isolates were constructed and their infectivity was tested by agroinfiltration on Nicotiana benthamiana and sap inoculation on Chinese cabbage and radish. In addition, we analyzed the phylogenetic relationship of six new Korean TuMV isolates and determined their respective affiliation with the four major groups. We also conducted recombination analysis for isolates recently occurring in Korean using RDP4 software, which provided new insight into the evolutionary relationships among Korean isolates of TuMV.

Download Full-text

Infectious Barley stripe mosaic virus RNA transcribed in Vitro from full-length genomic cDNA clones

Virology ◽

10.1016/0042-6822(89)90601-6 ◽

1989 ◽

Vol 171 (2) ◽

pp. 342-349 ◽

Cited By ~ 90

Author(s):

I.T.D. Petty ◽

B.G. Hunter ◽

N. Wei ◽

A.O. Jackson

Keyword(s):

Mosaic Virus ◽

Full Length ◽

Barley Stripe Mosaic Virus ◽

Cdna Clones ◽

Virus Rna ◽

Genomic Cdna

Download Full-text

Construction of an infectious full-length cDNA clone of potato aucuba mosaic virus

Archives of Virology ◽

10.1007/s00705-021-05018-w ◽

2021 ◽

Vol 166 (5) ◽

pp. 1427-1431

Author(s):

Buyang Chen ◽

Qi Lin ◽

Yueyan Yin ◽

Liangliang Jiang ◽

Fang Wang ◽

...

Keyword(s):

Mosaic Virus ◽

Cdna Clone ◽

Full Length ◽

Full Length Cdna ◽

Aucuba Mosaic

Download Full-text

A cassava common mosaic virus vector for virus-induced gene silencing in cassava

Plant Methods ◽

10.1186/s13007-021-00775-w ◽

2021 ◽

Vol 17 (1) ◽

Author(s):

Decai Tuo ◽

Peng Zhou ◽

Pu Yan ◽

Hongguang Cui ◽

Yang Liu ◽

...

Keyword(s):

Gene Silencing ◽

Mosaic Virus ◽

Gene Function ◽

Viral Vector ◽

Function Analysis ◽

Systemic Infection ◽

Cdna Clones ◽

Virus Induced Gene Silencing ◽

Efficient Gene ◽

Gene Function Analysis

Abstract Background Cassava is an important crop for food security and industry in the least-developed and developing countries. The completion of the cassava genome sequence and identification of large numbers of candidate genes by next-generation sequencing provide extensive resources for cassava molecular breeding and increase the need for rapid and efficient gene function analysis systems in cassava. Several plant virus-induced gene silencing (VIGS) systems have been developed as reverse genetic tools for rapid gene function analysis in cassava. However, these VIGS vectors could cause severe viral symptoms or inefficient gene silencing. Results In this study, we constructed agroinfection-compatible infectious cDNA clones of cassava common mosaic virus isolate CM (CsCMV-CM, genus Potexvirus, family Alphaflexiviridae) that causes systemic infection with mild symptoms in cassava. CsCMV-CM was then modified to a viral vector carrying the Nimble cloning frame, which facilitates the rapid and high-throughput cloning of silencing fragments into the viral genome. The CsCMV-based vector successfully silenced phytoene desaturase (PDS) and magnesium chelatase subunit I (ChlI) in different cassava varieties and Nicotiana benthamiana. The silencing of the ChlI gene could persist for more than two months. Conclusions This CsCMV-based VIGS system provides a new tool for rapid and efficient gene function studies in cassava.

Download Full-text