Construction of Full-Length Infectious Clones of Turnip Mosaic Virus Isolates Infecting Perilla Frutescens and Genetic Analysis of Recently Emerged Strains of Tumv in Korea

Abstract Perilla is an annual herb with a unique aroma and taste and has been cultivated in Korea for hundreds of years. Owing to the highly edible and medicinal value of Perilla plants, it has been widely cultivated in many Asian and European countries. Recently, several viruses have been reported to cause diseases in Perilla in Korea, including turnip mosaic virus (TuMV) which is known as a brassica pathogen due to its significant damage to brassica crops. In this study, we determined the complete genome sequences of two new TuMV isolates originating from Perilla in Korea. Full-length infectious cDNA clones of these two isolates were constructed and their infectivity was tested by agroinfiltration on Nicotiana benthamiana and sap inoculation on Chinese cabbage and radish. In addition, we analyzed the phylogenetic relationship of six new Korean TuMV isolates and determined their respective affiliation with the four major groups. We also conducted recombination analysis for isolates recently occurring in Korean using RDP4 software, which provided new insight into the evolutionary relationships among Korean isolates of TuMV.

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Construction of Full-length Infectious cDNA Clones of Two Korean Isolates of Turnip Mosaic Virus Breaking Resistance in Brassica Napus

10.21203/rs.3.rs-803814/v1 ◽

2021 ◽

Author(s):

Zheng-Xing Song ◽

Eun-Young Seo ◽

Wen-Xing Hu ◽

Jong-Hyeon Jeong ◽

Jae Sun Moon ◽

...

Keyword(s):

Brassica Napus ◽

South Korea ◽

Mosaic Virus ◽

Nicotiana Benthamiana ◽

Turnip Mosaic Virus ◽

Cdna Clones ◽

Infectious Clones ◽

The World ◽

Complete Genomes ◽

Systemic Symptoms

Abstract In this work, two new Turnip mosaic virus (TuMV) strains (Canola-12 and Canola-14) overcoming resistance in canola (Brassica napus) were isolated from a B. napus sample which showed typical TuMV-like symptoms and was collected from Gimcheon city, South Korea in 2020. Complete genomes and infectious clones of each isolate were obtained. Phylogenetic analysis indicated that the strains isolated from canola belonged to the World-B group. Both infectious clones which were driven by 35S and T7 promoters induced systemic symptoms on Nicotiana benthamiana and B. napus. To our knowledge, this is the first report of TuMV infecting B. napus in South Korea.

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Molecular and Biological Characterisation of Turnip mosaic virus Isolates Infecting Poppy (Papaver somniferum and P. rhoeas) in Slovakia

Viruses ◽

10.3390/v10080430 ◽

2018 ◽

Vol 10 (8) ◽

pp. 430 ◽

Cited By ~ 2

Author(s):

Miroslav Glasa ◽

Katarína Šoltys ◽

Lukáš Predajňa ◽

Nina Sihelská ◽

Slavomíra Nováková ◽

...

Keyword(s):

Mosaic Virus ◽

Phylogenetic Analyses ◽

Molecular Data ◽

Turnip Mosaic Virus ◽

Biological Assays ◽

Genetic Complexity ◽

The World ◽

History Of ◽

Virus Isolates

In recent years, the accumulated molecular data of Turnip mosaic virus (TuMV) isolates from various hosts originating from different parts of the world considerably helped to understand the genetic complexity and evolutionary history of the virus. In this work, four complete TuMV genomes (HC9, PK1, MS04, MS15) were characterised from naturally infected cultivated and wild-growing Papaver spp., hosts from which only very scarce data were available previously. Phylogenetic analyses showed the affiliation of Slovak Papaver isolates to the world-B and basal-B groups. The PK1 isolate showed a novel intra-lineage recombination pattern, further confirming the important role of recombination in the shaping of TuMV genetic diversity. Biological assays indicated that the intensity of symptoms in experimentally inoculated oilseed poppy are correlated to TuMV accumulation level in leaves. This is the first report of TuMV in poppy plants in Slovakia.

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Comparative Studies on Biological and Serological Properties of Turnip Mosaic Virus Isolates

Journal of the Faculty of Agriculture, Kyushu University ◽

10.5109/23716 ◽

1980 ◽

Vol 25 (1) ◽

pp. 15-23

Author(s):

Jang Kyung Choi ◽

Nobuaki Matsuyama ◽

Satoshi Wakimoto

Keyword(s):

Mosaic Virus ◽

Comparative Studies ◽

Turnip Mosaic Virus ◽

Virus Isolates

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Molecular Characterization of the 3'-Terminal Region of Turnip mosaic virus Isolates from Eastern China

Journal of Phytopathology ◽

10.1111/j.1439-0434.2007.01236.x ◽

2007 ◽

Vol 155 (6) ◽

pp. 333-341 ◽

Cited By ~ 7

Author(s):

Y.-P. Tian ◽

X.-P. Zhu ◽

J.-L. Liu ◽

X.-Q. Yu ◽

J. Du ◽

...

Keyword(s):

Mosaic Virus ◽

Molecular Characterization ◽

Eastern China ◽

Turnip Mosaic Virus ◽

Terminal Region ◽

Virus Isolates

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Construction of Full-Length cDNA Clones to Soil-Borne Wheat Mosaic Virus RNA1 and RNA2, from Which Infectious RNAs Are Transcribed In Vitro: Virion Formation and Systemic Infection without Expression of the N-Terminal and C-Terminal Extensions to the Capsid Protein

Virology ◽

10.1006/viro.2000.0587 ◽

2000 ◽

Vol 277 (1) ◽

pp. 66-75 ◽

Cited By ~ 17

Author(s):

Aya Yamamiya ◽

Yukio Shirako

Keyword(s):

Mosaic Virus ◽

Capsid Protein ◽

Systemic Infection ◽

Full Length ◽

Cdna Clones ◽

Full Length Cdna ◽

Virion Formation

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Sequence Variations Among 17 New Radish Isolates of Turnip mosaic virus Showing Differential Pathogenicity and Infectivity in Nicotiana benthamiana, Brassica rapa, and Raphanus sativus

Phytopathology ◽

10.1094/phyto-12-17-0401-r ◽

2019 ◽

Vol 109 (5) ◽

pp. 904-912 ◽

Cited By ~ 2

Author(s):

Junsu Gong ◽

Hye-Kyoung Ju ◽

Ik-Hyun Kim ◽

Eun-Young Seo ◽

In-Sook Cho ◽

...

Keyword(s):

Amino Acid ◽

Mosaic Virus ◽

Chinese Cabbage ◽

Nicotiana Benthamiana ◽

Turnip Mosaic Virus ◽

Amino Acid Residues ◽

Systemic Necrosis ◽

Infectious Clones ◽

Sequence Variations ◽

Distinct Sequence

Infectious clones were generated from 17 new Korean radish isolates of Turnip mosaic virus (TuMV). Phylogenetic analysis indicated that all new isolates, and three previously characterized Korean radish isolates, belong to the basal-BR group (indicating that the pathotype can infect both Brassica and Raphanus spp.). Pairwise analysis revealed genomic nucleotide and polyprotein amino acid identities of >87.9 and >95.7%, respectively. Five clones (HJY1, HJY2, KIH2, BE, and prior isolate R007) had lower sequence identities than other isolates and produced mild symptoms in Nicotiana benthamiana. These isolates formed three distinct sequence classes (HJY1/HJY2/R007, KIH2, and BE), and several differential amino acid residues (in P1, P3, 6K2, and VPg) were present only in mild isolates HJY1, HJY2, and R007. The remaining isolates all induced systemic necrosis in N. benthamiana. Four mild isolates formed a phylogenetic subclade separate from another subclade including all of the necrosis-inducing isolates plus mild isolate KIH2. Symptom severity in radish and Chinese cabbage genotypes was not correlated with pathogenicity in N. benthamiana; indeed, Chinese cabbage cultivar Norang was not infected by any isolate, whereas Chinese cabbage cultivar Chusarang was uniformly susceptible. Four isolates were unable to infect radish cultivar Iljin, but no specific amino acid residues were correlated with avirulence. These results may lead to the identification of new resistance genes against TuMV.

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