scholarly journals Protocol for single-cell isolation and genome amplification of environmental microbial eukaryotes for genomic analysis

2022 ◽  
Vol 3 (1) ◽  
pp. 100968
Author(s):  
Doina Ciobanu ◽  
Sandy Chan ◽  
Steven Ahrendt ◽  
C. Alisha Quandt ◽  
Gerald L. Benny ◽  
...  
2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Qiang Zhang ◽  
Tingting Wang ◽  
Qian Zhou ◽  
Peng Zhang ◽  
Yanhai Gong ◽  
...  

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Huichao Chai ◽  
Yongxiang Feng ◽  
Fei Liang ◽  
Wenhui Wang

Successful single-cell isolation is a pivotal technique for subsequent biological and chemical analysis of single cells. Although significant advances have been made in single-cell isolation and analysis techniques, most passive...


2014 ◽  
Vol 133 (2) ◽  
pp. AB142
Author(s):  
Neil Alexis ◽  
Heather Wells ◽  
Yogesh Saini ◽  
Louisa Brighton ◽  
Nancy Allbritton ◽  
...  

2016 ◽  
Vol 115 (2) ◽  
pp. 992-1002 ◽  
Author(s):  
Z. Navratilova ◽  
K. B. Godfrey ◽  
B. L. McNaughton

Neural recording technology is improving rapidly, allowing for the detection of spikes from hundreds of cells simultaneously. The limiting step in multielectrode electrophysiology continues to be single cell isolation. However, this step is crucial to the interpretation of data from putative single neurons. We present here, in simulation, an illustration of possibly erroneous conclusions that may be reached when poorly isolated single cell data are analyzed. Grid cells are neurons recorded in rodents, and bats, that spike in equally spaced locations in a hexagonal pattern. One theory states that grid firing patterns arise from a combination of band firing patterns. However, we show here that summing the grid firing patterns of two poorly resolved neurons can result in spurious band-like patterns. Thus, evidence of neurons spiking in band patterns must undergo extreme scrutiny before it is accepted. Toward this aim, we discuss single cell isolation methods and metrics.


Hybridoma ◽  
1983 ◽  
Vol 2 (2) ◽  
pp. 231-234 ◽  
Author(s):  
R.J. SIJENS ◽  
A.A.M. THOMAS ◽  
A. JACKERS ◽  
A. BOEYÉ

Micromachines ◽  
2022 ◽  
Vol 13 (1) ◽  
pp. 80
Author(s):  
Xiaohu Zhou ◽  
Han Wu ◽  
Haotian Wen ◽  
Bo Zheng

Single-cell analysis is becoming an indispensable tool in modern biological and medical research. Single-cell isolation is the key step for single-cell analysis. Single-cell printing shows several distinct advantages among the single-cell isolation techniques, such as precise deposition, high encapsulation efficiency, and easy recovery. Therefore, recent developments in single-cell printing have attracted extensive attention. We review herein the recently developed bioprinting strategies with single-cell resolution, with a special focus on inkjet-like single-cell printing. First, we discuss the common cell printing strategies and introduce several typical and advanced printing strategies. Then, we introduce several typical applications based on single-cell printing, from single-cell array screening and mass spectrometry-based single-cell analysis to three-dimensional tissue formation. In the last part, we discuss the pros and cons of the single-cell strategies and provide a brief outlook for single-cell printing.


2018 ◽  
Vol 33 (3) ◽  
pp. 464-473 ◽  
Author(s):  
Erica Silvestris ◽  
Paola Cafforio ◽  
Stella D’Oronzo ◽  
Claudia Felici ◽  
Franco Silvestris ◽  
...  

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