Mechanical role of actinotrichia in shaping the caudal fin of zebrafish

Genomic and Proteomic changes play a crucial role in perpetuating regeneration of complex tissues through differentiation and growth. The complex Epimorphic regeneration of zebrafish caudal fin tissue is hasty and absolute. This study was executed to understand the role of various genes/proteins involved in the regeneration of zebrafish caudal fin tissue through differential expression analysis. High throughput transcriptomics analysis involving Next Generation Sequencing approach and iTRAQ based quantitative proteomics analyses were performed on the regenerating tissue samples for various regenerating time points. Based on our study 1408 genes and 661 proteins were found differentially regulated in the regenerating caudal fin tissue for having at least 1-log fold change in their expression at 12hpa, 1, 2, 3 and 7dpa stages against control non-regenerating tissue. Interleukin, SLC, PRMT, HOX, neurotransmitter and several novel genes were found to be associated with regeneration for its differential regulation during the mechanism. Based on the network and pathway analysis the differentially regulated genes and proteins were found allied with activation of cell proliferation, cell viability, cell survival & cell movement and inactivation of organismal death, morbidity, necrosis, death of embryo & cell death. Network pathways such as Cancer & development disorder, Cell signaling molecular transport, organismal injury & abnormalities and Cellular development, growth & proliferation were found most significantly associated with the zebrafish caudal fin regeneration mechanism. This study has mapped a detailed insight of the genes/proteins expression associated with the epimorphic regeneration more profoundly.

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Role of annexin gene and its regulation during zebrafish caudal fin regeneration

Wound Repair and Regeneration ◽

10.1111/wrr.12429 ◽

2016 ◽

Vol 24 (3) ◽

pp. 551-559 ◽

Cited By ~ 12

Author(s):

Sandeep Saxena ◽

Sruthi Purushothaman ◽

Vuppalapaty Meghah ◽

Bhawna Bhatti ◽

Akhila Poruri ◽

...

Keyword(s):

Caudal Fin ◽

Fin Regeneration ◽

Annexin Gene

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The Role of Compliant Joint and Flexibility on the Propulsive Performance of a Self Propelled Caudal Fin

Volume 2: Mechanics and Behavior of Active Materials; Integrated System Design and Implementation; Bio-Inspired Materials and Systems; Energy Harvesting ◽

10.1115/smasis2012-8116 ◽

2012 ◽

Author(s):

Ashok K. Kancharala ◽

Michael K. Philen ◽

Mayuresh J. Patil

Keyword(s):

Beam Theory ◽

Leading Edge ◽

Maximum Efficiency ◽

Underwater Robotics ◽

Caudal Fin ◽

Fish Locomotion ◽

Propulsive Performance ◽

Wide Range ◽

Compliant Joint

Caudal fin plays an important role in the thrust generation of fish locomotion. Recent studies on the role of body flexibility in propulsion show that fish have a remarkable ability to control or modulate the stiffness of the fin for optimized propulsive performance. Along with the fin stiffness, the stiffness of the joint connecting the caudal peduncle and the fin also plays a major role in the generation of thrust. Since thrust and efficiency are dependent on various parameters, a detailed investigation would be required to understand the combined effect of fin and joint flexibility over a wide range of parameters for optimized performance. The present study provides a parametric study on the effect of flexibility of fin and the compliant joint on propulsive performance. For this investigation, fluid structure interaction of the fin has been modeled considering unsteady slender wing theory coupled with the nonlinear Euler-Bernoulli beam theory. The compliant joint has been modeled as a torsional spring at the leading edge of the fin. A comparison of Self–propelled speed (SPS) and efficiency with parameters such as heaving and pitching amplitude, oscillation frequency, flexibility of the fin and the compliant joint is reported. The model predicts the optimized stiffnesses of the compliant joint and the fin for maximum efficiency. These optimal stiffnesses vary with the motion parameters suggesting the benefits of active stiffness modulation in bio-inspired underwater robotics.

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Functional Role of Annexins in Zebrafish Caudal Fin Regeneration – A Gene Knockdown Approach in Regenerating Tissue

10.1101/342238 ◽

2018 ◽

Author(s):

Mir Quoseena ◽

Sowmya Vuppaladadium ◽

Shahid Hussain ◽

Swarna Bharathi ◽

Mohammed M Idris

Keyword(s):

Functional Role ◽

Target Genes ◽

Human Beings ◽

Caudal Fin ◽

Fin Regeneration ◽

Epimorphic Regeneration ◽

Knock Down ◽

Regeneration Mechanism ◽

Almost All

AbstractRegeneration is an adaptive phenomenon with wide biological implications spread heterogeneously in almost all the organism including human beings. The ability of regeneration varies from species to species for its impediment. Epimorphic regeneration of zebrafish caudal fin tissue is most widely studied regeneration mechanism for its discrete and rapid regenerative capability. Several genes/proteins were found to be associated with zebrafish caudal fin tissue regeneration. Here, we have evaluated the functional role ofAnnexin 2aand2bgenes in adult zebrafish caudal fin tissue undergoing regeneration using a novel CRSISPR-Cas9 gene knock down approach. Knock down of both the genes individually elicited in decelerated regeneration and down regulation of target genes and its partner genes/proteins such as ANXA1a, ANXA5b and ANXA13. This study validates a novel gene targeting approach and the possible role of annexin in regeneration mechanism.

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Inhibition of BMP and FGF signaling prior to wound epithelium formation leads to an aberrant regenerative response in teleost fish Poecilia latipinna

10.1101/2021.01.27.428424 ◽

2021 ◽

Author(s):

Isha Ranadive ◽

Sonam Patel ◽

Siddharth Pai ◽

Kashmira Khaire ◽

Suresh Balakrishnan

Keyword(s):

Cell Death ◽

Teleost Fish ◽

The Other ◽

Fgf Signaling ◽

Caudal Fin ◽

Fin Regeneration ◽

Blastema Formation ◽

Messenger Molecules ◽

Wound Epithelium

The BMP and FGF pathways play a pivotal role in the successful regeneration of caudal fin of teleost fish. Individual inhibition of these pathways led to impaired caudal fin regeneration until the pharmacologic inhibitor of FGF (SU5402) and BMP (LDN193189) were metabolized off. Therefore, in the current study both these pathways were inhibited collectively wherein inhibition of BMP and FGF during the wound epithelium formation led to stalling of the process by bringing down the established levels of shh and runx2. In members of the treatment group, it was observed that, each blastema grows crouched rather than linear and the regrown lepidotrichia therefore remain tilted down. Amongst the other irregularities observed, the transition from epithelial to mesenchymal cells was found hindered due to down-regulation of snail and twist, brought about by BMP and FGF inhibition. Compromised expression of Snail and twist deranged the normal levels of cadherins causing disruption in the transition of cells. Lastly, blocking BMP and FGF delayed blastema formation and proliferation due to diminished levels of fgf2, fgf8, fgf10 and bmp6, while casp3 and casp9 levels remained heightened causing accelerated cell death. This study not only highlights the axial role of BMP and FGF pathways in regeneration but also accentuates the collaboration amongst the two. This ingenious coordination of signalling further reinforces the involvement of relaying messenger molecules between these crucial pathways.

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