scholarly journals Functional Role of Annexins in Zebrafish Caudal Fin Regeneration – A Gene Knockdown Approach in Regenerating Tissue

2018 ◽  
Author(s):  
Mir Quoseena ◽  
Sowmya Vuppaladadium ◽  
Shahid Hussain ◽  
Swarna Bharathi ◽  
Mohammed M Idris
Keyword(s):  
Functional Role ◽  
Target Genes ◽  
Human Beings ◽  
Caudal Fin ◽  
Fin Regeneration ◽  
Epimorphic Regeneration ◽  
Knock Down ◽  
Regeneration Mechanism ◽  
Almost All

AbstractRegeneration is an adaptive phenomenon with wide biological implications spread heterogeneously in almost all the organism including human beings. The ability of regeneration varies from species to species for its impediment. Epimorphic regeneration of zebrafish caudal fin tissue is most widely studied regeneration mechanism for its discrete and rapid regenerative capability. Several genes/proteins were found to be associated with zebrafish caudal fin tissue regeneration. Here, we have evaluated the functional role ofAnnexin 2aand2bgenes in adult zebrafish caudal fin tissue undergoing regeneration using a novel CRSISPR-Cas9 gene knock down approach. Knock down of both the genes individually elicited in decelerated regeneration and down regulation of target genes and its partner genes/proteins such as ANXA1a, ANXA5b and ANXA13. This study validates a novel gene targeting approach and the possible role of annexin in regeneration mechanism.

scholarly journals Understanding the complexity of Epimorphic Regeneration in zebrafish: A Transcriptomic and Proteomic approach.

2021 ◽  
Author(s):  
Sarena Banu ◽  
Namami Gaur ◽  
Sowmy Nair ◽  
Tanuja Ravikrishnan ◽  
Shahida Khan ◽  
...  
Keyword(s):  
Differential Expression Analysis ◽  
Cell Movement ◽  
Molecular Transport ◽  
Caudal Fin ◽  
Tissue Samples ◽  
Fin Regeneration ◽  
Epimorphic Regeneration ◽  
Proteomic Approach ◽  
Generation Sequencing

Genomic and Proteomic changes play a crucial role in perpetuating regeneration of complex tissues through differentiation and growth. The complex Epimorphic regeneration of zebrafish caudal fin tissue is hasty and absolute. This study was executed to understand the role of various genes/proteins involved in the regeneration of zebrafish caudal fin tissue through differential expression analysis. High throughput transcriptomics analysis involving Next Generation Sequencing approach and iTRAQ based quantitative proteomics analyses were performed on the regenerating tissue samples for various regenerating time points. Based on our study 1408 genes and 661 proteins were found differentially regulated in the regenerating caudal fin tissue for having at least 1-log fold change in their expression at 12hpa, 1, 2, 3 and 7dpa stages against control non-regenerating tissue. Interleukin, SLC, PRMT, HOX, neurotransmitter and several novel genes were found to be associated with regeneration for its differential regulation during the mechanism. Based on the network and pathway analysis the differentially regulated genes and proteins were found allied with activation of cell proliferation, cell viability, cell survival & cell movement and inactivation of organismal death, morbidity, necrosis, death of embryo & cell death. Network pathways such as Cancer & development disorder, Cell signaling molecular transport, organismal injury & abnormalities and Cellular development, growth & proliferation were found most significantly associated with the zebrafish caudal fin regeneration mechanism. This study has mapped a detailed insight of the genes/proteins expression associated with the epimorphic regeneration more profoundly.

scholarly journals Functional role of annexins in zebrafish caudal fin regeneration – A gene knockdown approach in regenerating tissue

Biochimie ◽  
2020 ◽  
Vol 175 ◽  
pp. 125-131 ◽  
Author(s):  
Mir Quoseena ◽  
Sowmya Vuppaladadium ◽  
Shahid Hussain ◽  
Sarena Banu ◽  
Swarna Bharathi ◽  
...  
Keyword(s):  
Functional Role ◽  
Gene Knockdown ◽  
Caudal Fin ◽  
Fin Regeneration

Role of takrasevan in agnimandya - a review.

2019 ◽  
Vol 7 (4) ◽  
Author(s):  
Poonam Bharat Mandhare ◽  
Deepa R. Kale
Keyword(s):  
Eating Habits ◽  
Well Being ◽  
Daily Basis ◽  
Treatment Modalities ◽  
Human Beings ◽  
Diet And Exercise ◽  
Life Threatening ◽  
Almost All ◽  
Modern Era

Background: Our health and overall well-being mainly depends on our eating habits, physical activities, sleep patterns etc. Human beings, in order to adjust themselves in the modern era, have been compiled to become fast and mechanical. Due to this they can’t give proper attention to diet and exercise. Due to this Aaharpachan Kriya gets affected leading to Agnimandya (low or decreased digestive power). According to Ayurveda Agnimandya is responsible for almost all diseases. Objectives: Objective is to study importance of Takrasevan in Agnimandya.  Material and methods: Ayurvedic / modern texts and literature, research paper. Discussion: Grahani, Atisar, Arsh etc. are the commonest diseases seen in today’s life.  These diseases are not life threatening but can cause great distress to patient.  Ayurveda gave an amount of treatment modalities; one of them is Takrasevan i. e. consumption of buttermilk. Takra having ushna virya, kashaya rasa, madhur vipak and ruksha guna properties which pacifies Vata, Pitta, Kapha and potentiate the Agni and digests as well as absorbs the food articles and treats the disease in generous way. Conclusion: By this study it can be conclude that Takra (Buttermilk) can use on daily basis as dietary product. As it contains Probiotics which facilitates proper digestion and absorption process, it maintains proper metabolism to keep person free from diseases.   

Role of Hox genes in zebrafish caudal fin regeneration

2006 ◽  
Vol 25 ◽  
pp. S25-S25
Author(s):  
M SARRASJR ◽  
R THUMMEL ◽  
A GODMAN
Keyword(s):  
Hox Genes ◽  
Caudal Fin ◽  
Fin Regeneration

scholarly journals The Role of Education in Combating Violent Extremism in Developing Countries with an Emphasis on Afghanistan

2021 ◽  
Vol 9 (3) ◽  
pp. 273-287
Author(s):  
Mohammad Mohammadi
Keyword(s):  
Developing Countries ◽  
Inclusive Education ◽  
Process Integration ◽  
Human Beings ◽  
Equal Education ◽  
Group Life ◽  
Violent Extremism ◽  
The World ◽  
Almost All

Education as a pervasive process has affected all aspects of individual and group life, and today, almost all those seeking development and reform start from all over the world. Preventing radicalization and violent extremism is one of the most controversial issues in the world in this century. In addition, it was found that some extremist groups are recruiting in educational settings. These findings led to a new approach to the education sector as a prominent partner in preventing and combating the radicalization of young people. Education faces many challenges in developing countries, including Afghanistan. With the change of world views, cultures and different beliefs of human beings, the goals of education have also changed and according to this, there is a direct relationship and coordination between society and education. The inefficiency of the education system in different sections of the society will have adverse effects and dangerous consequences, as can be seen from the findings, lack of inclusive education, lack of quality in education and lack of job opportunities after graduation are recommended to strengthen the role of education for sustainable development in developing countries, especially Afghanistan because education is the cornerstone of development. One of the most important and influential institutions, investing in equal education and creating a safe environment for students at different levels (primary, secondary and tertiary) and adopting sensible strategies can be in the process. Integration, convergence, peaceful coexistence and the elimination of violence between peoples and nations have brought about many effects and changes.

scholarly journals Functional role of TRPC proteins in native systems: implications from knockout and knock-down studies

2005 ◽  
Vol 567 (1) ◽  
pp. 59-66 ◽  
Author(s):  
Marc Freichel ◽  
Rudi Vennekens ◽  
Jenny Olausson ◽  
Susanne Stolz ◽  
Stephan E. Philipp ◽  
...  
Keyword(s):  
Functional Role ◽  
Knock Down ◽  
Trpc Proteins

Role of annexin gene and its regulation during zebrafish caudal fin regeneration

2016 ◽  
Vol 24 (3) ◽  
pp. 551-559 ◽  
Author(s):  
Sandeep Saxena ◽  
Sruthi Purushothaman ◽  
Vuppalapaty Meghah ◽  
Bhawna Bhatti ◽  
Akhila Poruri ◽  
...  
Keyword(s):  
Caudal Fin ◽  
Fin Regeneration ◽  
Annexin Gene

scholarly journals Peranan Kepolisian Resor Tebing Tinggi Dalam Penegakan Hukum Terhadap Tindak Pidana Ujaran Kebencian Di Media Sosial

2021 ◽  
Vol 4 (2) ◽  
pp. 905-912
Author(s):  
Renal Eldinata Samosir ◽  
Taufik Siregar ◽  
Rizkan Zulyadi
Keyword(s):  
Hate Speech ◽  
Human Beings ◽  
Literature Study ◽  
Study Approach ◽  
Interview Method ◽  
Electronic Transactions ◽  
Police Data ◽  
Almost All ◽  
National Police

The presence of such sophisticated information technology has given a new nuance by touching almost all aspects of life. Technology has made it easy for people to carry out daily activities to meet their needs, as well as facilitate interaction between human beings wherever they are. This research is a normative juridical research that uses a literature study and field study approach. The data collection tool used in this study used the interview method. Interviews were conducted on informants or investigators at the Tebing Tinggi Resort Police. Data analysis was carried out qualitatively. The results of the study indicate that the role of the police in law enforcement against hate speech crimes on social media as regulated in Law no. 19 of 2016 concerning Amendments to Law No. 11 of 2008 concerning Information and Electronic Transactions (UU ITE) and Law no. 2 of 2002 concerning the Indonesian National Police. The role of the police includes receiving reports or complaints about the occurrence of an event that is reasonably suspected of being a criminal act; it is obliged to immediately carry out the necessary investigative actions; As an investigator who knows, receives a report or complaint about the occurrence of an event that is reasonably suspected to be a criminal act, he is obliged to immediately carry out the necessary investigative actions and find and collect evidence so that the case becomes clear so that the suspect can be found.

scholarly journals Inhibition of BMP and FGF signaling prior to wound epithelium formation leads to an aberrant regenerative response in teleost fish Poecilia latipinna

2021 ◽  
Author(s):  
Isha Ranadive ◽  
Sonam Patel ◽  
Siddharth Pai ◽  
Kashmira Khaire ◽  
Suresh Balakrishnan
Keyword(s):  
Cell Death ◽  
Teleost Fish ◽  
The Other ◽  
Fgf Signaling ◽  
Caudal Fin ◽  
Fin Regeneration ◽  
Blastema Formation ◽  
Messenger Molecules ◽  
Wound Epithelium

The BMP and FGF pathways play a pivotal role in the successful regeneration of caudal fin of teleost fish. Individual inhibition of these pathways led to impaired caudal fin regeneration until the pharmacologic inhibitor of FGF (SU5402) and BMP (LDN193189) were metabolized off. Therefore, in the current study both these pathways were inhibited collectively wherein inhibition of BMP and FGF during the wound epithelium formation led to stalling of the process by bringing down the established levels of shh and runx2. In members of the treatment group, it was observed that, each blastema grows crouched rather than linear and the regrown lepidotrichia therefore remain tilted down. Amongst the other irregularities observed, the transition from epithelial to mesenchymal cells was found hindered due to down-regulation of snail and twist, brought about by BMP and FGF inhibition. Compromised expression of Snail and twist deranged the normal levels of cadherins causing disruption in the transition of cells. Lastly, blocking BMP and FGF delayed blastema formation and proliferation due to diminished levels of fgf2, fgf8, fgf10 and bmp6, while casp3 and casp9 levels remained heightened causing accelerated cell death. This study not only highlights the axial role of BMP and FGF pathways in regeneration but also accentuates the collaboration amongst the two. This ingenious coordination of signalling further reinforces the involvement of relaying messenger molecules between these crucial pathways.

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