Lab-on-a-chip platforms for quantification of multicellular interactions in bone remodeling

2018 ◽  
Vol 365 (1) ◽  
pp. 106-118 ◽  
Author(s):  
Estee L. George ◽  
Sharon L. Truesdell ◽  
Spencer L. York ◽  
Marnie M. Saunders
Keyword(s):  
Bone Remodeling ◽  
Lab On A Chip ◽  
Multicellular Interactions

scholarly journals Cellular considerations for optimizing bone cell culture and remodeling in a lab-on-a-chip platform

BioTechniques ◽  
2020 ◽  
Vol 68 (5) ◽  
pp. 263-269 ◽  
Author(s):  
Sharon L Truesdell ◽  
Estee L George ◽  
Marnie M Saunders
Keyword(s):  
Cell Culture ◽  
Bone Formation ◽  
Bone Remodeling ◽  
Functional Activity ◽  
Bone Cells ◽  
Lab On A Chip ◽  
Bone Cell ◽  
Cell Seeding ◽  
Bone Cell Culture

Our lab has developed a lab-on-a-chip platform for bone remodeling that enables long-term culturing of bone cells out to 7 weeks and serves as a foundation toward a multicellular organ-on-a-chip system. Here, we optimized culturing protocols for osteoblasts, osteoclasts and osteocytes within the lab-on-a-chip and performed functional activity assays for quantifying bone formation and resorption. We analyzed cell seeding densities, feeding schedules and time in culture as a basis for optimizing culturing protocols. Further, we addressed concerns of sterility, cytotoxicity and leakage during the extended culture period within the polydimethylsiloxane chip. This system provides a method for quantifying the soluble effects of mechanically stimulated osteocytes on bone remodeling (formation/resorption).

A Lab-On-A-Chip Platform for Stimulating Osteocyte Mechanotransduction and Analyzing Functional Outcomes of Bone Remodeling

10.3791/61076 ◽  
2020 ◽  
Author(s):  
Sharon L. Truesdell ◽  
Estee L. George ◽  
Christopher C. Van Vranken ◽  
Marnie M. Saunders
Keyword(s):  
Bone Remodeling ◽  
Functional Outcomes ◽  
Lab On A Chip

Does short-term administration of glucagon-like peptide type 2 affect bone mass and markers of bone remodeling in short bowel patients?

2001 ◽  
Vol 120 (5) ◽  
pp. A314-A314
Author(s):  
K HADERSLEV ◽  
P JEPPESEN ◽  
B HARTMANN ◽  
J THULESEN ◽  
J GRAFF ◽  
...  
Keyword(s):  
Bone Mass ◽  
Bone Remodeling ◽  
Short Term ◽  
Short Bowel ◽  
Term Administration ◽  
Glucagon Like Peptide

Bioactive Sclerostin: better analyte definition in the measurement of bone remodeling

2019 ◽  
Author(s):  
A Bitzer ◽  
J Wallwitz ◽  
E Gadermaier ◽  
G Berg ◽  
G Himmler
Keyword(s):  
Bone Remodeling

Dickkopf-1 is dispensable for thyroid hormone-induced changes in bone remodeling

2019 ◽  
Author(s):  
E Tsourdi ◽  
J Colditz ◽  
F Lademann ◽  
E Rijntjes ◽  
J Köhrle ◽  
...  
Keyword(s):  
Thyroid Hormone ◽  
Bone Remodeling ◽  
Induced Changes ◽  
Dickkopf 1

Genetic aspects of bone remodeling disturbance in patients with aggressive periodontitis

2013 ◽  
Author(s):  
Anastasia Zinovyeva ◽  
Victoria Atrushkevich ◽  
Alexander Polyakov
Keyword(s):  
Bone Remodeling ◽  
Aggressive Periodontitis

Inhibition of RANKL-mediated bone remodeling decreases bone damage and improves strength in response to fatigue loading

2014 ◽  
Author(s):  
Nicolas Bonnet ◽  
Maude Gerbaix ◽  
Paul Kostenuik ◽  
Mike Ominsky ◽  
Serge Ferrari
Keyword(s):  
Bone Remodeling ◽  
Fatigue Loading ◽  
Bone Damage
Sign in / Sign up

Export Citation Format

Share Document