Identification and analysis of the proximal promoters of microRNA genes in Arabidopsis

Background: Learning and memory is basic aspects in neurogenetics as most of the neurological disorders start with dementia or memory loss. Several genes associated with memory formation have been discovered. MicroRNA genes miR-1000 and miR-375 were reported to be associated with neural integration and glucose homeostasis in some insects and vertebrates. However, neuronal function of these genes is yet to be established in D. melanogaster. Objective: Possible role of miR-1000 and miR-375 in learning and memory formation in this fly has been explored in the present study. Methods: Both appetitive and aversive olfactory conditional learning were tested in the miR-1000 and miR-375 knockout (KO) strains and compared with wild one. Five days old third instar larvae were trained by allowing them to be associated with an odor with reward (fructose) or punishment (salt). Then, the larvae were tested to calculate their preferences to the odor trained with. Learning index (LI) values and larval locomotion speed were calculated for all strains. Results: No significant difference was observed for larval locomotion speed in mutant strains. Knockout strain of miR-1000 showed significant deficiency in both appetitive and aversive memory formation whereas miR-375 KO strain showed a significantly lower response only in appetitive one. Conclusion: The results of the present study indicate important role played by these two genes in forming short-term memory in D. melanogaster.

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Biased hosting of intronic microRNA genes

Bioinformatics ◽

10.1093/bioinformatics/btq077 ◽

2010 ◽

Vol 26 (8) ◽

pp. 992-995 ◽

Cited By ~ 18

Author(s):

David Golan ◽

Carmit Levy ◽

Brad Friedman ◽

Noam Shomron

Keyword(s):

Microrna Genes

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Pollen Specificity Elements Reside in 30 bp of the Proximal Promoters of Two Pollen-Expressed Genes

The Plant Cell ◽

10.2307/3869858 ◽

1995 ◽

Vol 7 (3) ◽

pp. 373

Author(s):

Yoram Eyal ◽

Catherine Curie ◽

Sheila McCormick

Keyword(s):

Proximal Promoters

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Systematic discovery and characterization of stress-related microRNA genes in Oryza sativa

Biologia ◽

10.1515/biolog-2015-0001 ◽

2015 ◽

Vol 70 (1) ◽

Cited By ~ 1

Author(s):

Kai Bin Xie ◽

Xue Zhou ◽

Tian Hai Zhang ◽

Bao Long Zhang ◽

Li Ming Chen ◽

...

Keyword(s):

Abiotic Stress ◽

Stress Responses ◽

Systematic Investigation ◽

Chemical Toxicity ◽

Promoter Regions ◽

Crop Species ◽

Translation Inhibition ◽

Microrna Genes ◽

Insight Into

AbstractAbiotic stresses including drought, salinity, extreme temperatures, chemical toxicity and oxidative are the natural status of the environment to exert serious threats to agriculture. Abiotic stress-related microRNAs (ASmiRNAs) are a group of microRNAs (miRNAs) regulating stress responses in plants. However, the systematic investigation of ASmiRNAs is limited in Rice (O. sativa), a typical abiotic stress-resistant crop species. In the present work, we systematically investigated ASmiRNAs in silico. First, we identified 177 putative ASmiRNAs in O.sativa. Second, we found most ASmiRNAs were driven by TATA-promoter and most stress-related miRNA promoter regions contained the stress-related elements. Third, we found many ASmiRNAs families were species/family specific and a set of miRNAs might derive from genomic repeat-sequences in O. sativa. Finally, we found the ASmiRNAs in O. sativa target 289 genes with 1050 predicted target sites in which 98% sites have cleavage activity and 2% sites have translation inhibition activity. In conclusion, our findings provide an insight into both the function and evolution of ASmiRNAs and improve our understanding on the mechanism of abiotic stress resistance in O. sativa.

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