Artificially designed routes for the conversion of starch to value-added mannosyl compounds through coupling in vitro and in vivo metabolic engineering strategies

2020 ◽  
Vol 61 ◽  
pp. 215-224
Author(s):  
Chaoyu Tian ◽  
Jiangang Yang ◽  
Yunjie Li ◽  
Tong Zhang ◽  
Jiao Li ◽  
...  
Keyword(s):  
Metabolic Engineering ◽  
Value Added

scholarly journals Fruit Seeds as Sources of Bioactive Compounds: Sustainable Production of High Value-Added Ingredients from By-Products within Circular Economy

Molecules ◽  
2019 ◽  
Vol 24 (21) ◽  
pp. 3854 ◽  
Author(s):  
Fidelis ◽  
Moura ◽  
Kabbas Junior ◽  
Pap ◽  
Mattila ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Circular Economy ◽  
Raw Materials ◽  
Scale Up ◽  
Sustainable Use ◽  
Value Added ◽  
Water Soluble ◽  
Lipophilic Compounds

The circular economy is an umbrella concept that applies different mechanisms aiming to minimize waste generation, thus decoupling economic growth from natural resources. Each year, an estimated one-third of all food produced is wasted; this is equivalent to 1.3 billion tons of food, which is worth around US$1 trillion or even $2.6 trillion when social and economic costs are included. In the fruit and vegetable sector, 45% of the total produced amount is lost in the production (post-harvest, processing, and distribution) and consumption chains. Therefore, it is necessary to find new technological and environmentally friendly solutions to utilize fruit wastes as new raw materials to develop and scale up the production of high value-added products and ingredients. Considering that the production and consumption of fruits has increased in the last years and following the need to find the sustainable use of different fruit side streams, this work aimed to describe the chemical composition and bioactivity of different fruit seeds consumed worldwide. A comprehensive focus is given on the extraction techniques of water-soluble and lipophilic compounds and in vitro/in vivo functionalities, and the link between chemical composition and observed activity is holistically explained.

scholarly journals Developing Clostridia as Cell Factories for Short- and Medium-Chain Ester Production

2021 ◽  
Vol 9 ◽  
Author(s):  
Qingzhuo Wang ◽  
Naief H. Al Makishah ◽  
Qi Li ◽  
Yanan Li ◽  
Wenzheng Liu ◽  
...  
Keyword(s):  
Organic Acids ◽  
Value Added ◽  
Carbon Chain ◽  
Chain Elongation ◽  
Medium Chain ◽  
Cell Factories ◽  
Alcohol Acyltransferase ◽  
Volatile Esters

Short- and medium-chain volatile esters with flavors and fruity fragrances, such as ethyl acetate, butyl acetate, and butyl butyrate, are usually value-added in brewing, food, and pharmacy. The esters can be naturally produced by some microorganisms. As ester-forming reactions are increasingly deeply understood, it is possible to produce esters in non-natural but more potential hosts. Clostridia are a group of important industrial microorganisms since they can produce a variety of volatile organic acids and alcohols with high titers, especially butanol and butyric acid through the CoA-dependent carbon chain elongation pathway. This implies sufficient supplies of acyl-CoA, organic acids, and alcohols in cells, which are precursors for ester production. Besides, some Clostridia could utilize lignocellulosic biomass, industrial off-gas, or crude glycerol to produce other branched or straight-chain alcohols and acids. Therefore, Clostridia offer great potential to be engineered to produce short- and medium-chain volatile esters. In the review, the efforts to produce esters from Clostridia via in vitro lipase-mediated catalysis and in vivo alcohol acyltransferase (AAT)-mediated reaction are comprehensively revisited. Besides, the advantageous characteristics of several Clostridia and clostridial consortia for bio-ester production and the driving force of synthetic biology to clostridial chassis development are also discussed. It is believed that synthetic biotechnology should enable the future development of more effective Clostridia for ester production.

scholarly journals Outer membrane vesicles catabolize lignin-derived aromatic compounds in Pseudomonas putida KT2440

2020 ◽  
Vol 117 (17) ◽  
pp. 9302-9310 ◽  
Author(s):  
Davinia Salvachúa ◽  
Allison Z. Werner ◽  
Isabel Pardo ◽  
Martyna Michalska ◽  
Brenna A. Black ◽  
...  
Keyword(s):  
Pseudomonas Putida ◽  
Outer Membrane ◽  
Aromatic Compounds ◽  
Value Added ◽  
Membrane Vesicles ◽  
Outer Membrane Vesicles ◽  
Pseudomonas Putida Kt2440 ◽  
Rich Media

Lignin is an abundant and recalcitrant component of plant cell walls. While lignin degradation in nature is typically attributed to fungi, growing evidence suggests that bacteria also catabolize this complex biopolymer. However, the spatiotemporal mechanisms for lignin catabolism remain unclear. Improved understanding of this biological process would aid in our collective knowledge of both carbon cycling and microbial strategies to valorize lignin to value-added compounds. Here, we examine lignin modifications and the exoproteome of three aromatic–catabolic bacteria: Pseudomonas putida KT2440, Rhodoccocus jostii RHA1, and Amycolatopsis sp. ATCC 39116. P. putida cultivation in lignin-rich media is characterized by an abundant exoproteome that is dynamically and selectively packaged into outer membrane vesicles (OMVs). Interestingly, many enzymes known to exhibit activity toward lignin-derived aromatic compounds are enriched in OMVs from early to late stationary phase, corresponding to the shift from bioavailable carbon to oligomeric lignin as a carbon source. In vivo and in vitro experiments demonstrate that enzymes contained in the OMVs are active and catabolize aromatic compounds. Taken together, this work supports OMV-mediated catabolism of lignin-derived aromatic compounds as an extracellular strategy for nutrient acquisition by soil bacteria and suggests that OMVs could potentially be useful tools for synthetic biology and biotechnological applications.

scholarly journals Biosynthesis of dendroketose from different carbon sources using in vitro and in vivo metabolic engineering strategies

2018 ◽  
Vol 11 (1) ◽  
Author(s):  
Jiangang Yang ◽  
Yueming Zhu ◽  
Ge Qu ◽  
Yan Zeng ◽  
Chaoyu Tian ◽  
...  
Keyword(s):  
Metabolic Engineering ◽  
Carbon Sources

scholarly journals A Patatin-Like Protein Associated with the Polyhydroxyalkanoate (PHA) Granules of Haloferax mediterranei Acts as an Efficient Depolymerase in the Degradation of Native PHA

2015 ◽  
Vol 81 (9) ◽  
pp. 3029-3038 ◽  
Author(s):  
Guiming Liu ◽  
Jing Hou ◽  
Shuangfeng Cai ◽  
Dahe Zhao ◽  
Lei Cai ◽  
...  
Keyword(s):  
Value Added ◽  
Degradation Pathway ◽  
Site Directed Mutagenesis ◽  
Haloferax Mediterranei ◽  
Content Type ◽  
New Strategy ◽  
Key Enzyme ◽  
Shed Light

ABSTRACTThe key enzymes and pathways involved in polyhydroxyalkanoate (PHA) biosynthesis in haloarchaea have been identified in recent years, but the haloarchaeal enzymes for PHA degradation remain unknown. In this study, a patatin-like PHA depolymerase, PhaZh1, was determined to be located on the PHA granules in the haloarchaeonHaloferax mediterranei. PhaZh1 hydrolyzed the native PHA (nPHA) [including native polyhydroxybutyrate (nPHB) and native poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (nPHBV) in this study] granulesin vitrowith 3-hydroxybutyrate (3HB) monomer as the primary product. The site-directed mutagenesis of PhaZh1 indicated that Gly16, Ser47(in a classical lipase box, G-X-S47-X-G), and Asp195of this depolymerase were essential for its activity in nPHA granule hydrolysis. Notably,phaZh1andbdhA(encoding putative 3HB dehydrogenase) form a gene cluster (HFX_6463to_6464) inH. mediterranei. The 3HB monomer generated from nPHA degradation by PhaZh1 could be further converted into acetoacetate by BdhA, indicating that PhaZh1-BdhA may constitute the first part of a PHA degradation pathwayin vivo. Interestingly, although PhaZh1 showed efficient activity and was most likely the key enzyme in nPHA granule hydrolysisin vitro, the knockout ofphaZh1had no significant effect on the intracellular PHA mobilization, implying the existence of an alternative PHA mobilization pathway(s) that functions effectively within the cells ofH. mediterranei. Therefore, identification of this patatin-like depolymerase of haloarchaea may provide a new strategy for producing the high-value-added chiral compound (R)-3HB and may also shed light on the PHA mobilization in haloarchaea.

Upgrade of wood sugar d-xylose to a value-added nutraceutical by in vitro metabolic engineering

2019 ◽  
Vol 52 ◽  
pp. 1-8 ◽  
Author(s):  
Kun Cheng ◽  
Wenming Zheng ◽  
Hongge Chen ◽  
Yi-Heng P. Job Zhang
Keyword(s):  
Metabolic Engineering ◽  
Value Added ◽  
A Value

Efficient Bioconversion of Sucrose to High‐Value‐Added Glucaric Acid by In Vitro Metabolic Engineering

ChemSusChem ◽  
2019 ◽  
Vol 12 (10) ◽  
pp. 2278-2285 ◽  
Author(s):  
Hui‐Hui Su ◽  
Ze‐Wang Guo ◽  
Xiao‐Ling Wu ◽  
Pei Xu ◽  
Ning Li ◽  
...  
Keyword(s):  
Metabolic Engineering ◽  
Value Added ◽  
Glucaric Acid
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