scholarly journals Fluorescent probes with high pKa values based on traditional, near-infrared rhodamine, and hemicyanine fluorophores for sensitive detection of lysosomal pH variations

Methods ◽  
2019 ◽  
Vol 168 ◽  
pp. 40-50 ◽  
Author(s):  
Wafa Mazi ◽  
Rashmi Adhikari ◽  
Yibin Zhang ◽  
Shuai Xia ◽  
Mingxi Fang ◽  
...  
Keyword(s):  
Fluorescent Probes ◽  
Near Infrared ◽  
Sensitive Detection ◽  
Pka Values ◽  
Lysosomal Ph ◽  
Ph Variations

Near-infrared fluorescent probes based on piperazine-functionalized BODIPY dyes for sensitive detection of lysosomal pH

2015 ◽  
Vol 3 (10) ◽  
pp. 2173-2184 ◽  
Author(s):  
Jingtuo Zhang ◽  
Mu Yang ◽  
Cong Li ◽  
Nethaniah Dorh ◽  
Fei Xie ◽  
...  
Keyword(s):  
Fluorescent Probes ◽  
Near Infrared ◽  
Living Cells ◽  
Sensitive Detection ◽  
Lysosomal Ph

Three BODIPY-based near-infrared fluorescent probes have been synthesized, characterized, and evaluated for the sensing and imaging of lysosomal pH inside living cells.

scholarly journals Near-infrared fluorescent probes based on TBET and FRET rhodamine acceptors with different pKa values for sensitive ratiometric visualization of pH changes in live cells

2019 ◽  
Vol 7 (2) ◽  
pp. 198-209 ◽  
Author(s):  
Jianbo Wang ◽  
Shuai Xia ◽  
Jianheng Bi ◽  
Yibin Zhang ◽  
Mingxi Fang ◽  
...  
Keyword(s):  
Fluorescent Probes ◽  
Near Infrared ◽  
Live Cells ◽  
Pka Values ◽  
Ph Changes ◽  
Ph Variations

Three near-infrared ratiometric fluorescent probes with different pKa values were synthesized to achieve sensitive ratiometric visualization of pH variations in lysosomes.

pH-activatable near-infrared fluorescent probes for detection of lysosomal pH inside living cells

2014 ◽  
Vol 2 (28) ◽  
pp. 4500-4508 ◽  
Author(s):  
Giri K. Vegesna ◽  
Jagadeesh Janjanam ◽  
Jianheng Bi ◽  
Fen-Tair Luo ◽  
Jingtuo Zhang ◽  
...  
Keyword(s):  
Fluorescent Probes ◽  
Near Infrared ◽  
Living Cells ◽  
Lysosomal Ph

Four near-infrared fluorescent probes have been synthesized, characterized, and evaluated for detection of lysosomal pH inside living cells.

scholarly journals Ratiometric Near-Infrared Fluorescent Probes Based on Hemicyanine Dyes Bearing Dithioacetal and Formal Residues for pH Detection in Mitochondria

Molecules ◽  
2021 ◽  
Vol 26 (7) ◽  
pp. 2088
Author(s):  
Yunnan Yan ◽  
Yibin Zhang ◽  
Shuai Xia ◽  
Shulin Wan ◽  
Tara Vohs ◽  
...  
Keyword(s):  
Fluorescent Probes ◽  
Near Infrared ◽  
Formyl Group ◽  
Pka Values ◽  
Theoretical Methods ◽  
Hemicyanine Dye ◽  
Hemicyanine Dyes ◽  
Experimental Values ◽  
Van Der Waals Radii ◽  
Ph Detection

Ratiometric near-infrared fluorescent probes (AH+ and BH+) have been prepared for pH determination in mitochondria by attaching dithioacetal and formal residues onto a hemicyanine dye. The reactive formyl group on probe BH+ allows for retention inside mitochondria as it can react with a protein primary amine residue to form an imine under slightly basic pH 8.0. Probes AH+ and BH+ display ratiometric fluorescent responses to pH changes through the protonation and deprotonaton of a hydroxy group in hemicyanine dyes with experimentally determined pKa values of 6.85 and 6.49, respectively. Calculated pKa values from a variety of theoretical methods indicated that the SMDBONDI method of accounting for solvent and van der Waals radii plus including a water molecule located near the site of protonation produced the closest overall agreement with the experimental values at 7.33 and 6.14 for AH+ and BH+ respectively.

scholarly journals Near-infrared fluorescent probes with BODIPY donors and rhodamine and merocyanine acceptors for ratiometric determination of lysosomal pH variance

2019 ◽  
Vol 294 ◽  
pp. 1-13 ◽  
Author(s):  
Shuai Xia ◽  
Mingxi Fang ◽  
Jianbo Wang ◽  
Jianheng Bi ◽  
Wafa Mazi ◽  
...  
Keyword(s):  
Fluorescent Probes ◽  
Near Infrared ◽  
Lysosomal Ph

Near-infrared fluorescent probes with higher quantum yields and neutral pKa values for the evaluation of intracellular pH

RSC Advances ◽  
2016 ◽  
Vol 6 (73) ◽  
pp. 69641-69646 ◽  
Author(s):  
Xinbo Song ◽  
Mingyu Hu ◽  
Chao Wang ◽  
Yi Xiao
Keyword(s):  
High Resolution ◽  
Intracellular Ph ◽  
Fluorescent Probes ◽  
Near Infrared ◽  
Quantum Yields ◽  
Pka Values

Near-infrared fluorescent probes for pH, named pH-A and pH-B, for labeling cells to produce high resolution fluorescent images reflect the changes of intracellular pH.

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